Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS06635

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS06625 and BWI76_RS06630 are separated by 220 nucleotidesBWI76_RS06630 and BWI76_RS06635 are separated by 37 nucleotidesBWI76_RS06635 and BWI76_RS06640 are separated by 65 nucleotides BWI76_RS06625: BWI76_RS06625 - acyl-CoA thioesterase II, at 1,392,936 to 1,393,811 _RS06625 BWI76_RS06630: BWI76_RS06630 - hypothetical protein, at 1,394,032 to 1,394,541 _RS06630 BWI76_RS06635: BWI76_RS06635 - methylated-DNA--protein-cysteine methyltransferase, at 1,394,579 to 1,394,890 _RS06635 BWI76_RS06640: BWI76_RS06640 - hypothetical protein, at 1,394,956 to 1,395,363 _RS06640 Position (kb) 1394 1395Strain fitness (log2 ratio) -2 -1 0 1at 1393.667 kb on + strand, within BWI76_RS06625at 1393.675 kb on - strand, within BWI76_RS06625at 1393.811 kb on + strandat 1393.871 kb on - strandat 1393.872 kb on - strandat 1393.934 kb on + strandat 1393.944 kb on + strandat 1393.992 kb on + strandat 1394.151 kb on + strand, within BWI76_RS06630at 1394.325 kb on + strand, within BWI76_RS06630at 1394.387 kb on + strand, within BWI76_RS06630at 1394.439 kb on - strand, within BWI76_RS06630at 1394.531 kb on - strandat 1394.627 kb on + strand, within BWI76_RS06635at 1394.735 kb on + strand, within BWI76_RS06635at 1394.744 kb on - strand, within BWI76_RS06635at 1395.082 kb on - strand, within BWI76_RS06640at 1395.101 kb on + strand, within BWI76_RS06640at 1395.192 kb on + strand, within BWI76_RS06640at 1395.192 kb on + strand, within BWI76_RS06640at 1395.197 kb on + strand, within BWI76_RS06640at 1395.197 kb on + strand, within BWI76_RS06640at 1395.210 kb on - strand, within BWI76_RS06640at 1395.224 kb on - strand, within BWI76_RS06640at 1395.328 kb on + strandat 1395.336 kb on - strandat 1395.718 kb on + strandat 1395.732 kb on + strandat 1395.758 kb on + strandat 1395.766 kb on - strandat 1395.766 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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1,393,667 + BWI76_RS06625 0.83 +0.2
1,393,675 - BWI76_RS06625 0.84 -0.3
1,393,811 + +1.2
1,393,871 - +0.8
1,393,872 - -0.1
1,393,934 + +0.5
1,393,944 + +0.2
1,393,992 + +0.4
1,394,151 + BWI76_RS06630 0.23 -0.6
1,394,325 + BWI76_RS06630 0.57 -0.6
1,394,387 + BWI76_RS06630 0.70 +0.4
1,394,439 - BWI76_RS06630 0.80 -1.9
1,394,531 - +0.9
1,394,627 + BWI76_RS06635 0.15 -1.2
1,394,735 + BWI76_RS06635 0.50 -2.5
1,394,744 - BWI76_RS06635 0.53 -0.1
1,395,082 - BWI76_RS06640 0.31 -0.4
1,395,101 + BWI76_RS06640 0.36 +0.5
1,395,192 + BWI76_RS06640 0.58 -1.4
1,395,192 + BWI76_RS06640 0.58 -0.2
1,395,197 + BWI76_RS06640 0.59 +1.2
1,395,197 + BWI76_RS06640 0.59 -0.4
1,395,210 - BWI76_RS06640 0.62 -0.7
1,395,224 - BWI76_RS06640 0.66 +0.7
1,395,328 + -0.4
1,395,336 - -1.5
1,395,718 + -0.5
1,395,732 + -0.2
1,395,758 + -1.1
1,395,766 - -1.8
1,395,766 - +0.1

Or see this region's nucleotide sequence