Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06425 and BWI76_RS06430 overlap by 2 nucleotides BWI76_RS06430 and BWI76_RS06435 are separated by 229 nucleotides BWI76_RS06435 and BWI76_RS06440 are separated by 10 nucleotides BWI76_RS06440 and BWI76_RS06445 are separated by 65 nucleotides
BWI76_RS06425: BWI76_RS06425 - MFS transporter, at 1,350,342 to 1,351,742
_RS06425
BWI76_RS06430: BWI76_RS06430 - hypothetical protein, at 1,351,741 to 1,351,920
_RS06430
BWI76_RS06435: BWI76_RS06435 - MFS transporter, at 1,352,150 to 1,353,514
_RS06435
BWI76_RS06440: BWI76_RS06440 - AP endonuclease, at 1,353,525 to 1,354,343
_RS06440
BWI76_RS06445: BWI76_RS06445 - FAD-dependent oxidoreductase, at 1,354,409 to 1,356,112
_RS06445
Position (kb) 1352
1353
1354 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 1351.164 kb on - strand, within BWI76_RS06425 at 1351.189 kb on + strand, within BWI76_RS06425 at 1351.290 kb on + strand, within BWI76_RS06425 at 1351.549 kb on - strand, within BWI76_RS06425 at 1351.605 kb on - strand at 1351.769 kb on + strand at 1351.770 kb on + strand, within BWI76_RS06430 at 1351.778 kb on - strand, within BWI76_RS06430 at 1351.788 kb on - strand, within BWI76_RS06430 at 1351.802 kb on + strand, within BWI76_RS06430 at 1351.802 kb on + strand, within BWI76_RS06430 at 1351.810 kb on - strand, within BWI76_RS06430 at 1351.810 kb on - strand, within BWI76_RS06430 at 1351.810 kb on - strand, within BWI76_RS06430 at 1351.942 kb on - strand at 1351.944 kb on - strand at 1351.959 kb on + strand at 1352.168 kb on - strand at 1352.391 kb on + strand, within BWI76_RS06435 at 1352.435 kb on + strand, within BWI76_RS06435 at 1352.564 kb on - strand, within BWI76_RS06435 at 1353.498 kb on - strand at 1354.045 kb on + strand, within BWI76_RS06440 at 1354.360 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,351,164 - BWI76_RS06425 0.59 -0.0 1,351,189 + BWI76_RS06425 0.60 +0.4 1,351,290 + BWI76_RS06425 0.68 +0.4 1,351,549 - BWI76_RS06425 0.86 -0.1 1,351,605 - -0.1 1,351,769 + -1.1 1,351,770 + BWI76_RS06430 0.16 +0.3 1,351,778 - BWI76_RS06430 0.21 +0.1 1,351,788 - BWI76_RS06430 0.26 +0.6 1,351,802 + BWI76_RS06430 0.34 +0.1 1,351,802 + BWI76_RS06430 0.34 -0.9 1,351,810 - BWI76_RS06430 0.38 -0.5 1,351,810 - BWI76_RS06430 0.38 +0.7 1,351,810 - BWI76_RS06430 0.38 +0.2 1,351,942 - -1.9 1,351,944 - -1.9 1,351,959 + -0.3 1,352,168 - -1.8 1,352,391 + BWI76_RS06435 0.18 +3.1 1,352,435 + BWI76_RS06435 0.21 +0.7 1,352,564 - BWI76_RS06435 0.30 +0.8 1,353,498 - +1.4 1,354,045 + BWI76_RS06440 0.63 +0.6 1,354,360 + -0.5
Or see this region's nucleotide sequence