Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05140

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05130 and BWI76_RS05135 overlap by 1 nucleotidesBWI76_RS05135 and BWI76_RS05140 overlap by 4 nucleotidesBWI76_RS05140 and BWI76_RS05145 are separated by 118 nucleotides BWI76_RS05130: BWI76_RS05130 - iron-hydroxamate transporter ATP-binding subunit, at 1,078,385 to 1,079,182 _RS05130 BWI76_RS05135: BWI76_RS05135 - iron-hydroxamate transporter substrate-binding subunit, at 1,079,182 to 1,080,072 _RS05135 BWI76_RS05140: BWI76_RS05140 - Fe3+-hydroxamate ABC transporter permease FhuB, at 1,080,069 to 1,082,051 _RS05140 BWI76_RS05145: BWI76_RS05145 - aspartate aminotransferase family protein, at 1,082,170 to 1,083,450 _RS05145 Position (kb) 1080 1081 1082 1083Strain fitness (log2 ratio) -1 0 1at 1079.425 kb on + strand, within BWI76_RS05135at 1079.502 kb on - strand, within BWI76_RS05135at 1081.107 kb on + strand, within BWI76_RS05140at 1081.190 kb on - strand, within BWI76_RS05140at 1081.242 kb on - strand, within BWI76_RS05140at 1081.299 kb on + strand, within BWI76_RS05140at 1081.338 kb on - strand, within BWI76_RS05140at 1081.557 kb on + strand, within BWI76_RS05140at 1082.143 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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1,079,425 + BWI76_RS05135 0.27 +0.3
1,079,502 - BWI76_RS05135 0.36 -0.4
1,081,107 + BWI76_RS05140 0.52 +0.4
1,081,190 - BWI76_RS05140 0.57 +1.3
1,081,242 - BWI76_RS05140 0.59 +0.3
1,081,299 + BWI76_RS05140 0.62 +0.1
1,081,338 - BWI76_RS05140 0.64 -1.7
1,081,557 + BWI76_RS05140 0.75 +0.4
1,082,143 - -0.1

Or see this region's nucleotide sequence