Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04535 and BWI76_RS04540 are separated by 244 nucleotides BWI76_RS04540 and BWI76_RS04545 are separated by 30 nucleotides BWI76_RS04545 and BWI76_RS04550 overlap by 11 nucleotides
BWI76_RS04535: BWI76_RS04535 - LPS assembly protein LptD, at 937,884 to 940,226
_RS04535
BWI76_RS04540: BWI76_RS04540 - molecular chaperone DjlA, at 940,471 to 941,298
_RS04540
BWI76_RS04545: BWI76_RS04545 - two-component response regulator for citrate, at 941,329 to 942,027
_RS04545
BWI76_RS04550: BWI76_RS04550 - two-component sensor kinase, at 942,017 to 943,642
_RS04550
Position (kb) 940
941
942 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 940.300 kb on - strand at 940.316 kb on - strand at 940.336 kb on + strand at 940.348 kb on + strand at 940.356 kb on - strand at 940.356 kb on - strand at 940.440 kb on - strand at 940.484 kb on - strand at 940.540 kb on + strand at 940.548 kb on - strand at 940.559 kb on + strand, within BWI76_RS04540 at 940.564 kb on + strand, within BWI76_RS04540 at 940.651 kb on - strand, within BWI76_RS04540 at 941.159 kb on + strand, within BWI76_RS04540 at 941.167 kb on - strand, within BWI76_RS04540 at 941.174 kb on - strand, within BWI76_RS04540 at 941.187 kb on - strand, within BWI76_RS04540 at 941.189 kb on - strand, within BWI76_RS04540 at 941.264 kb on + strand at 941.422 kb on + strand, within BWI76_RS04545 at 941.432 kb on - strand, within BWI76_RS04545 at 941.586 kb on - strand, within BWI76_RS04545 at 941.653 kb on + strand, within BWI76_RS04545 at 941.693 kb on + strand, within BWI76_RS04545 at 941.742 kb on + strand, within BWI76_RS04545 at 941.829 kb on + strand, within BWI76_RS04545 at 942.066 kb on + strand at 942.141 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 940,300 - -0.2 940,316 - -2.7 940,336 + +0.2 940,348 + -0.6 940,356 - +0.5 940,356 - -1.6 940,440 - +0.2 940,484 - +0.4 940,540 + -0.5 940,548 - -0.4 940,559 + BWI76_RS04540 0.11 -0.0 940,564 + BWI76_RS04540 0.11 -2.7 940,651 - BWI76_RS04540 0.22 -0.3 941,159 + BWI76_RS04540 0.83 -0.3 941,167 - BWI76_RS04540 0.84 -2.0 941,174 - BWI76_RS04540 0.85 -1.6 941,187 - BWI76_RS04540 0.86 -0.0 941,189 - BWI76_RS04540 0.87 +0.1 941,264 + +1.0 941,422 + BWI76_RS04545 0.13 +0.2 941,432 - BWI76_RS04545 0.15 -3.0 941,586 - BWI76_RS04545 0.37 +1.3 941,653 + BWI76_RS04545 0.46 -1.4 941,693 + BWI76_RS04545 0.52 -1.0 941,742 + BWI76_RS04545 0.59 +0.3 941,829 + BWI76_RS04545 0.72 -0.1 942,066 + +2.1 942,141 - -0.7
Or see this region's nucleotide sequence