Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04095

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04085 and BWI76_RS04090 overlap by 32 nucleotidesBWI76_RS04090 and BWI76_RS04095 are separated by 14 nucleotidesBWI76_RS04095 and BWI76_RS04100 are separated by 91 nucleotides BWI76_RS04085: BWI76_RS04085 - DNA polymerase III subunit psi, at 841,445 to 841,858 _RS04085 BWI76_RS04090: BWI76_RS04090 - ribosomal-protein-alanine N-acetyltransferase RimI, at 841,827 to 842,273 _RS04090 BWI76_RS04095: BWI76_RS04095 - dUMP phosphatase, at 842,288 to 842,965 _RS04095 BWI76_RS04100: BWI76_RS04100 - peptide chain release factor 3, at 843,057 to 844,646 _RS04100 Position (kb) 842 843Strain fitness (log2 ratio) -1 0 1 2at 841.857 kb on + strandat 841.983 kb on + strand, within BWI76_RS04090at 842.066 kb on + strand, within BWI76_RS04090at 842.076 kb on - strand, within BWI76_RS04090at 842.128 kb on + strand, within BWI76_RS04090at 842.143 kb on + strand, within BWI76_RS04090at 842.730 kb on - strand, within BWI76_RS04095at 842.876 kb on - strand, within BWI76_RS04095at 843.799 kb on - strand, within BWI76_RS04100at 843.868 kb on + strand, within BWI76_RS04100

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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841,857 + +0.6
841,983 + BWI76_RS04090 0.35 +0.0
842,066 + BWI76_RS04090 0.53 +0.5
842,076 - BWI76_RS04090 0.56 -0.9
842,128 + BWI76_RS04090 0.67 -0.8
842,143 + BWI76_RS04090 0.71 +0.2
842,730 - BWI76_RS04095 0.65 +1.4
842,876 - BWI76_RS04095 0.87 +1.5
843,799 - BWI76_RS04100 0.47 +2.4
843,868 + BWI76_RS04100 0.51 -0.6

Or see this region's nucleotide sequence