Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02845 and BWI76_RS02850 are separated by 94 nucleotides BWI76_RS02850 and BWI76_RS02855 are separated by 36 nucleotides BWI76_RS02855 and BWI76_RS02860 are separated by 419 nucleotides
BWI76_RS02845: BWI76_RS02845 - hypothetical protein, at 583,181 to 583,732
_RS02845
BWI76_RS02850: BWI76_RS02850 - metalloprotease PmbA, at 583,827 to 585,179
_RS02850
BWI76_RS02855: BWI76_RS02855 - cytochrome B562, at 585,216 to 585,602
_RS02855
BWI76_RS02860: BWI76_RS02860 - glycine dehydrogenase, at 586,022 to 586,360
_RS02860
Position (kb) 583
584
585
586 Strain fitness (log2 ratio) -1
0
1 at 582.963 kb on + strand at 583.801 kb on + strand at 583.866 kb on - strand at 583.876 kb on + strand at 583.886 kb on - strand at 584.057 kb on - strand, within BWI76_RS02850 at 584.202 kb on - strand, within BWI76_RS02850 at 584.204 kb on + strand, within BWI76_RS02850 at 584.441 kb on + strand, within BWI76_RS02850 at 584.449 kb on - strand, within BWI76_RS02850 at 584.509 kb on + strand, within BWI76_RS02850 at 584.516 kb on - strand, within BWI76_RS02850 at 584.800 kb on + strand, within BWI76_RS02850 at 584.808 kb on - strand, within BWI76_RS02850 at 585.139 kb on + strand at 585.305 kb on - strand, within BWI76_RS02855 at 585.328 kb on - strand, within BWI76_RS02855 at 585.480 kb on - strand, within BWI76_RS02855 at 585.568 kb on - strand at 585.981 kb on + strand at 585.981 kb on + strand at 586.011 kb on - strand at 586.055 kb on - strand at 586.112 kb on + strand, within BWI76_RS02860
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 582,963 + +0.8 583,801 + +0.5 583,866 - +1.5 583,876 + -1.6 583,886 - -0.1 584,057 - BWI76_RS02850 0.17 -0.5 584,202 - BWI76_RS02850 0.28 +0.4 584,204 + BWI76_RS02850 0.28 -0.3 584,441 + BWI76_RS02850 0.45 +0.7 584,449 - BWI76_RS02850 0.46 +0.4 584,509 + BWI76_RS02850 0.50 -1.7 584,516 - BWI76_RS02850 0.51 -0.5 584,800 + BWI76_RS02850 0.72 +0.4 584,808 - BWI76_RS02850 0.73 +0.6 585,139 + -0.9 585,305 - BWI76_RS02855 0.23 -0.5 585,328 - BWI76_RS02855 0.29 -1.3 585,480 - BWI76_RS02855 0.68 +1.5 585,568 - -0.6 585,981 + +0.6 585,981 + -1.1 586,011 - +0.8 586,055 - +0.1 586,112 + BWI76_RS02860 0.27 -0.9
Or see this region's nucleotide sequence