Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02845

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02840 and BWI76_RS02845 are separated by 620 nucleotidesBWI76_RS02845 and BWI76_RS02850 are separated by 94 nucleotides BWI76_RS02840: BWI76_RS02840 - methylmalonate-semialdehyde dehydrogenase (CoA acylating), at 581,049 to 582,560 _RS02840 BWI76_RS02845: BWI76_RS02845 - hypothetical protein, at 583,181 to 583,732 _RS02845 BWI76_RS02850: BWI76_RS02850 - metalloprotease PmbA, at 583,827 to 585,179 _RS02850 Position (kb) 583 584Strain fitness (log2 ratio) -1 0 1 2 3at 582.216 kb on + strand, within BWI76_RS02840at 582.382 kb on - strand, within BWI76_RS02840at 582.402 kb on - strand, within BWI76_RS02840at 582.433 kb on - strandat 582.436 kb on + strandat 582.436 kb on + strandat 582.436 kb on + strandat 582.436 kb on + strandat 582.455 kb on - strandat 582.455 kb on - strandat 582.475 kb on + strandat 582.484 kb on - strandat 582.487 kb on + strandat 582.524 kb on - strandat 582.548 kb on + strandat 582.573 kb on + strandat 582.608 kb on + strandat 582.616 kb on + strandat 582.676 kb on + strandat 582.731 kb on - strandat 582.963 kb on + strandat 583.801 kb on + strandat 583.866 kb on - strandat 583.876 kb on + strandat 583.886 kb on - strandat 584.057 kb on - strand, within BWI76_RS02850at 584.202 kb on - strand, within BWI76_RS02850at 584.204 kb on + strand, within BWI76_RS02850at 584.441 kb on + strand, within BWI76_RS02850at 584.449 kb on - strand, within BWI76_RS02850at 584.509 kb on + strand, within BWI76_RS02850at 584.516 kb on - strand, within BWI76_RS02850

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
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582,216 + BWI76_RS02840 0.77 +0.4
582,382 - BWI76_RS02840 0.88 -0.1
582,402 - BWI76_RS02840 0.89 +2.5
582,433 - -1.1
582,436 + -1.1
582,436 + +1.4
582,436 + +0.5
582,436 + +0.2
582,455 - +1.5
582,455 - +1.3
582,475 + +0.5
582,484 - +0.6
582,487 + +0.8
582,524 - -0.4
582,548 + +0.1
582,573 + +0.1
582,608 + +3.5
582,616 + -1.1
582,676 + -0.5
582,731 - +0.6
582,963 + +0.8
583,801 + +0.5
583,866 - +1.5
583,876 + -1.6
583,886 - -0.1
584,057 - BWI76_RS02850 0.17 -0.5
584,202 - BWI76_RS02850 0.28 +0.4
584,204 + BWI76_RS02850 0.28 -0.3
584,441 + BWI76_RS02850 0.45 +0.7
584,449 - BWI76_RS02850 0.46 +0.4
584,509 + BWI76_RS02850 0.50 -1.7
584,516 - BWI76_RS02850 0.51 -0.5

Or see this region's nucleotide sequence