Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02215 and BWI76_RS02220 overlap by 1 nucleotides BWI76_RS02220 and BWI76_RS02225 overlap by 4 nucleotides BWI76_RS02225 and BWI76_RS02230 are separated by 0 nucleotides BWI76_RS02230 and BWI76_RS02235 are separated by 204 nucleotides
BWI76_RS02215: BWI76_RS02215 - carbon-phosphorus lyase complex subunit PhnI, at 458,357 to 459,421
_RS02215
BWI76_RS02220: BWI76_RS02220 - carbon-phosphorus lyase subunit PhnH, at 459,421 to 460,005
_RS02220
BWI76_RS02225: BWI76_RS02225 - phosphonate C-P lyase system protein PhnG, at 460,002 to 460,454
_RS02225
BWI76_RS02230: BWI76_RS02230 - phosphonate metabolism transcriptional regulator PhnF, at 460,455 to 461,180
_RS02230
BWI76_RS02235: BWI76_RS02235 - VOC family protein, at 461,385 to 461,819
_RS02235
Position (kb) 460
461 Strain fitness (log2 ratio) -2
-1
0
1
2 at 459.224 kb on - strand, within BWI76_RS02215 at 459.427 kb on - strand at 459.794 kb on + strand, within BWI76_RS02220 at 459.804 kb on + strand, within BWI76_RS02220 at 459.906 kb on + strand, within BWI76_RS02220 at 460.008 kb on - strand at 460.181 kb on - strand, within BWI76_RS02225 at 460.182 kb on - strand, within BWI76_RS02225 at 460.319 kb on + strand, within BWI76_RS02225 at 460.342 kb on + strand, within BWI76_RS02225 at 460.546 kb on - strand, within BWI76_RS02230 at 460.654 kb on - strand, within BWI76_RS02230 at 460.691 kb on + strand, within BWI76_RS02230 at 460.718 kb on + strand, within BWI76_RS02230 at 460.744 kb on + strand, within BWI76_RS02230 at 460.750 kb on - strand, within BWI76_RS02230 at 460.752 kb on - strand, within BWI76_RS02230 at 460.762 kb on - strand, within BWI76_RS02230 at 460.821 kb on + strand, within BWI76_RS02230 at 460.890 kb on - strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.027 kb on + strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.035 kb on - strand, within BWI76_RS02230 at 461.196 kb on + strand at 461.409 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 459,224 - BWI76_RS02215 0.81 -0.0 459,427 - -1.9 459,794 + BWI76_RS02220 0.64 +0.3 459,804 + BWI76_RS02220 0.65 -0.2 459,906 + BWI76_RS02220 0.83 +0.8 460,008 - +0.5 460,181 - BWI76_RS02225 0.40 +1.1 460,182 - BWI76_RS02225 0.40 +2.1 460,319 + BWI76_RS02225 0.70 +0.3 460,342 + BWI76_RS02225 0.75 -0.3 460,546 - BWI76_RS02230 0.13 -0.9 460,654 - BWI76_RS02230 0.27 +0.9 460,691 + BWI76_RS02230 0.33 -0.1 460,718 + BWI76_RS02230 0.36 +0.1 460,744 + BWI76_RS02230 0.40 -0.9 460,750 - BWI76_RS02230 0.41 -0.3 460,752 - BWI76_RS02230 0.41 -0.9 460,762 - BWI76_RS02230 0.42 -0.2 460,821 + BWI76_RS02230 0.50 -0.2 460,890 - BWI76_RS02230 0.60 +0.0 461,027 + BWI76_RS02230 0.79 -1.5 461,027 + BWI76_RS02230 0.79 -0.2 461,035 - BWI76_RS02230 0.80 -1.6 461,035 - BWI76_RS02230 0.80 +0.4 461,196 + +0.9 461,409 - +0.2
Or see this region's nucleotide sequence