Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02175 and BWI76_RS02180 are separated by 69 nucleotides BWI76_RS02180 and BWI76_RS02185 are separated by 33 nucleotides BWI76_RS02185 and BWI76_RS02190 are separated by 14 nucleotides BWI76_RS02190 and BWI76_RS02195 overlap by 1 nucleotides
BWI76_RS02175: BWI76_RS02175 - ribose 5-phosphate isomerase B, at 452,524 to 452,973
_RS02175
BWI76_RS02180: BWI76_RS02180 - hypothetical protein, at 453,043 to 453,375
_RS02180
BWI76_RS02185: BWI76_RS02185 - phosphonate metabolism protein PhnP, at 453,409 to 454,167
_RS02185
BWI76_RS02190: BWI76_RS02190 - ribose 1,5-bisphosphokinase, at 454,182 to 454,745
_RS02190
BWI76_RS02195: BWI76_RS02195 - alpha-D-ribose 1-methylphosphonate 5-triphosphate diphosphatase, at 454,745 to 455,881
_RS02195
Position (kb) 453
454
455 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 452.413 kb on + strand at 452.566 kb on - strand at 452.611 kb on - strand at 452.664 kb on - strand, within BWI76_RS02175 at 452.786 kb on - strand, within BWI76_RS02175 at 452.813 kb on - strand, within BWI76_RS02175 at 452.841 kb on - strand, within BWI76_RS02175 at 452.869 kb on - strand, within BWI76_RS02175 at 453.005 kb on + strand at 453.018 kb on - strand at 453.023 kb on + strand at 453.051 kb on + strand at 453.224 kb on - strand, within BWI76_RS02180 at 453.304 kb on + strand, within BWI76_RS02180 at 453.504 kb on + strand at 453.537 kb on + strand, within BWI76_RS02185 at 453.705 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.803 kb on - strand, within BWI76_RS02185 at 453.830 kb on + strand, within BWI76_RS02185 at 453.848 kb on - strand, within BWI76_RS02185 at 453.943 kb on + strand, within BWI76_RS02185 at 454.169 kb on - strand at 454.274 kb on + strand, within BWI76_RS02190 at 454.343 kb on + strand, within BWI76_RS02190 at 454.559 kb on + strand, within BWI76_RS02190 at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.695 kb on + strand at 454.763 kb on - strand at 454.797 kb on + strand at 454.920 kb on - strand at 454.931 kb on + strand, within BWI76_RS02195 at 455.083 kb on - strand, within BWI76_RS02195 at 455.091 kb on - strand, within BWI76_RS02195
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 452,413 + -0.1 452,566 - +0.1 452,611 - +1.4 452,664 - BWI76_RS02175 0.31 -0.5 452,786 - BWI76_RS02175 0.58 -0.3 452,813 - BWI76_RS02175 0.64 +0.5 452,841 - BWI76_RS02175 0.70 -0.1 452,869 - BWI76_RS02175 0.77 -0.3 453,005 + -2.2 453,018 - -0.4 453,023 + -0.8 453,051 + +0.2 453,224 - BWI76_RS02180 0.54 +0.1 453,304 + BWI76_RS02180 0.78 -0.9 453,504 + +1.0 453,537 + BWI76_RS02185 0.17 -0.3 453,705 - BWI76_RS02185 0.39 +0.9 453,803 - BWI76_RS02185 0.52 -0.2 453,803 - BWI76_RS02185 0.52 +0.4 453,830 + BWI76_RS02185 0.55 +0.4 453,848 - BWI76_RS02185 0.58 +0.2 453,943 + BWI76_RS02185 0.70 +4.3 454,169 - +0.5 454,274 + BWI76_RS02190 0.16 -3.0 454,343 + BWI76_RS02190 0.29 -0.3 454,559 + BWI76_RS02190 0.67 +0.1 454,695 + +0.4 454,695 + +0.1 454,695 + -0.4 454,695 + -0.2 454,763 - -1.7 454,797 + -0.2 454,920 - +0.4 454,931 + BWI76_RS02195 0.16 +0.6 455,083 - BWI76_RS02195 0.30 -2.2 455,091 - BWI76_RS02195 0.30 +0.2
Or see this region's nucleotide sequence