Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01660 and BWI76_RS01665 are separated by 31 nucleotides BWI76_RS01665 and BWI76_RS01670 are separated by 101 nucleotides
BWI76_RS01660: BWI76_RS01660 - malate synthase A, at 336,918 to 338,519
_RS01660
BWI76_RS01665: BWI76_RS01665 - Isocitrate lyase (EC 4.1.3.1) <small>(from data)</small>, at 338,551 to 339,855
_RS01665
BWI76_RS01670: BWI76_RS01670 - bifunctional isocitrate dehydrogenase kinase/phosphatase, at 339,957 to 341,699
_RS01670
Position (kb) 338
339
340 Strain fitness (log2 ratio) -2
-1
0
1
2 at 337.629 kb on - strand, within BWI76_RS01660 at 337.650 kb on - strand, within BWI76_RS01660 at 337.717 kb on + strand, within BWI76_RS01660 at 337.826 kb on + strand, within BWI76_RS01660 at 337.870 kb on - strand, within BWI76_RS01660 at 338.149 kb on - strand, within BWI76_RS01660 at 338.334 kb on + strand, within BWI76_RS01660 at 338.609 kb on + strand at 339.333 kb on - strand, within BWI76_RS01665 at 339.344 kb on + strand, within BWI76_RS01665 at 339.487 kb on + strand, within BWI76_RS01665 at 339.493 kb on + strand, within BWI76_RS01665 at 339.493 kb on + strand, within BWI76_RS01665 at 339.671 kb on - strand, within BWI76_RS01665 at 339.781 kb on - strand at 339.811 kb on - strand at 339.820 kb on + strand at 339.820 kb on + strand at 339.820 kb on + strand at 339.975 kb on - strand at 340.081 kb on + strand at 340.113 kb on - strand at 340.198 kb on + strand, within BWI76_RS01670 at 340.211 kb on - strand, within BWI76_RS01670 at 340.285 kb on + strand, within BWI76_RS01670 at 340.494 kb on - strand, within BWI76_RS01670 at 340.701 kb on + strand, within BWI76_RS01670 at 340.757 kb on + strand, within BWI76_RS01670 at 340.785 kb on + strand, within BWI76_RS01670 at 340.805 kb on + strand, within BWI76_RS01670
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 337,629 - BWI76_RS01660 0.44 +1.2 337,650 - BWI76_RS01660 0.46 -0.9 337,717 + BWI76_RS01660 0.50 -2.3 337,826 + BWI76_RS01660 0.57 -0.1 337,870 - BWI76_RS01660 0.59 +0.4 338,149 - BWI76_RS01660 0.77 -0.3 338,334 + BWI76_RS01660 0.88 -1.0 338,609 + +0.0 339,333 - BWI76_RS01665 0.60 +0.5 339,344 + BWI76_RS01665 0.61 +0.1 339,487 + BWI76_RS01665 0.72 +0.2 339,493 + BWI76_RS01665 0.72 +1.0 339,493 + BWI76_RS01665 0.72 +0.2 339,671 - BWI76_RS01665 0.86 +2.1 339,781 - +0.3 339,811 - +0.6 339,820 + -0.1 339,820 + -1.1 339,820 + -0.1 339,975 - +0.5 340,081 + -0.3 340,113 - +1.5 340,198 + BWI76_RS01670 0.14 +1.1 340,211 - BWI76_RS01670 0.15 +0.2 340,285 + BWI76_RS01670 0.19 -0.6 340,494 - BWI76_RS01670 0.31 +0.5 340,701 + BWI76_RS01670 0.43 -0.2 340,757 + BWI76_RS01670 0.46 +0.3 340,785 + BWI76_RS01670 0.48 -0.9 340,805 + BWI76_RS01670 0.49 +0.2
Or see this region's nucleotide sequence