Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27370 and BWI76_RS27375 overlap by 23 nucleotides BWI76_RS27375 and BWI76_RS27380 are separated by 174 nucleotides BWI76_RS27380 and BWI76_RS27385 are separated by 124 nucleotides BWI76_RS27385 and BWI76_RS27390 are separated by 65 nucleotides
BWI76_RS27370: BWI76_RS27370 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase, at 5,679,856 to 5,681,067
_RS27370
BWI76_RS27375: BWI76_RS27375 - deoxyuridine 5'-triphosphate nucleotidohydrolase, at 5,681,045 to 5,681,503
_RS27375
BWI76_RS27380: BWI76_RS27380 - nucleoid occlusion factor SlmA, at 5,681,678 to 5,682,274
_RS27380
BWI76_RS27385: BWI76_RS27385 - orotate phosphoribosyltransferase, at 5,682,399 to 5,683,040
_RS27385
BWI76_RS27390: BWI76_RS27390 - ribonuclease PH, at 5,683,106 to 5,683,822
_RS27390
Position (kb) 5681
5682
5683 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 5681.619 kb on - strand at 5681.767 kb on + strand, within BWI76_RS27380 at 5681.770 kb on + strand, within BWI76_RS27380 at 5682.119 kb on + strand, within BWI76_RS27380 at 5682.161 kb on + strand, within BWI76_RS27380 at 5682.193 kb on - strand, within BWI76_RS27380 at 5682.350 kb on + strand at 5682.357 kb on + strand at 5682.423 kb on + strand at 5682.695 kb on - strand, within BWI76_RS27385 at 5682.695 kb on - strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.916 kb on + strand, within BWI76_RS27385 at 5682.924 kb on - strand, within BWI76_RS27385 at 5682.924 kb on - strand, within BWI76_RS27385 at 5682.924 kb on - strand, within BWI76_RS27385 at 5683.011 kb on + strand at 5683.056 kb on - strand at 5683.142 kb on + strand at 5683.155 kb on + strand at 5683.194 kb on - strand, within BWI76_RS27390
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 5,681,619 - +2.3 5,681,767 + BWI76_RS27380 0.15 -0.2 5,681,770 + BWI76_RS27380 0.15 -2.3 5,682,119 + BWI76_RS27380 0.74 -0.6 5,682,161 + BWI76_RS27380 0.81 -0.5 5,682,193 - BWI76_RS27380 0.86 -1.0 5,682,350 + -0.1 5,682,357 + +0.1 5,682,423 + -0.8 5,682,695 - BWI76_RS27385 0.46 +0.1 5,682,695 - BWI76_RS27385 0.46 -1.1 5,682,916 + BWI76_RS27385 0.81 +1.1 5,682,916 + BWI76_RS27385 0.81 -3.2 5,682,916 + BWI76_RS27385 0.81 +0.5 5,682,916 + BWI76_RS27385 0.81 -0.1 5,682,916 + BWI76_RS27385 0.81 -1.9 5,682,916 + BWI76_RS27385 0.81 -0.4 5,682,916 + BWI76_RS27385 0.81 -1.2 5,682,924 - BWI76_RS27385 0.82 -1.3 5,682,924 - BWI76_RS27385 0.82 -1.2 5,682,924 - BWI76_RS27385 0.82 -1.7 5,683,011 + -2.9 5,683,056 - -0.1 5,683,142 + -0.9 5,683,155 + -2.3 5,683,194 - BWI76_RS27390 0.12 -0.8
Or see this region's nucleotide sequence