Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23525 and BWI76_RS23530 are separated by 80 nucleotides BWI76_RS23530 and BWI76_RS23535 are separated by 83 nucleotides BWI76_RS23535 and BWI76_RS23540 are separated by 149 nucleotides
BWI76_RS23525: BWI76_RS23525 - EamA family transporter, at 4,907,539 to 4,908,429
_RS23525
BWI76_RS23530: BWI76_RS23530 - MarR family transcriptional regulator, at 4,908,510 to 4,909,016
_RS23530
BWI76_RS23535: BWI76_RS23535 - hypothetical protein, at 4,909,100 to 4,909,561
_RS23535
BWI76_RS23540: BWI76_RS23540 - putative lysine exporter protein, at 4,909,711 to 4,910,322
_RS23540
Position (kb) 4909
4910 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 4908.128 kb on + strand, within BWI76_RS23525 at 4908.193 kb on - strand, within BWI76_RS23525 at 4908.522 kb on + strand at 4908.525 kb on - strand at 4908.527 kb on + strand at 4908.630 kb on - strand, within BWI76_RS23530 at 4908.742 kb on - strand, within BWI76_RS23530 at 4908.880 kb on + strand, within BWI76_RS23530 at 4908.987 kb on + strand at 4909.019 kb on - strand at 4909.091 kb on + strand at 4909.099 kb on - strand at 4909.161 kb on - strand, within BWI76_RS23535 at 4909.193 kb on - strand, within BWI76_RS23535 at 4909.247 kb on - strand, within BWI76_RS23535 at 4909.692 kb on + strand at 4909.705 kb on - strand at 4909.705 kb on - strand at 4909.705 kb on - strand at 4909.783 kb on + strand, within BWI76_RS23540 at 4910.010 kb on - strand, within BWI76_RS23540 at 4910.046 kb on - strand, within BWI76_RS23540 at 4910.130 kb on + strand, within BWI76_RS23540 at 4910.170 kb on - strand, within BWI76_RS23540 at 4910.187 kb on + strand, within BWI76_RS23540 at 4910.262 kb on + strand at 4910.291 kb on + strand at 4910.301 kb on - strand at 4910.313 kb on + strand at 4910.313 kb on + strand at 4910.328 kb on + strand at 4910.328 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.329 kb on + strand at 4910.336 kb on - strand at 4910.336 kb on - strand at 4910.336 kb on - strand at 4910.337 kb on - strand at 4910.337 kb on - strand at 4910.337 kb on - strand at 4910.348 kb on + strand at 4910.353 kb on + strand at 4910.353 kb on + strand at 4910.356 kb on - strand at 4910.361 kb on - strand at 4910.361 kb on - strand at 4910.361 kb on - strand at 4910.361 kb on - strand at 4910.361 kb on - strand at 4910.361 kb on - strand at 4910.361 kb on - strand at 4910.417 kb on - strand at 4910.417 kb on - strand at 4910.417 kb on - strand at 4910.417 kb on - strand at 4910.417 kb on - strand at 4910.417 kb on - strand at 4910.421 kb on + strand at 4910.429 kb on - strand at 4910.429 kb on - strand at 4910.429 kb on - strand at 4910.433 kb on - strand at 4910.439 kb on - strand at 4910.445 kb on + strand at 4910.445 kb on + strand at 4910.450 kb on - strand at 4910.453 kb on + strand at 4910.458 kb on - strand at 4910.488 kb on + strand at 4910.488 kb on + strand at 4910.495 kb on - strand at 4910.496 kb on - strand at 4910.498 kb on + strand at 4910.516 kb on - strand at 4910.516 kb on - strand at 4910.516 kb on - strand at 4910.521 kb on - strand at 4910.521 kb on - strand at 4910.521 kb on - strand at 4910.521 kb on - strand at 4910.541 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,908,128 + BWI76_RS23525 0.66 -0.3 4,908,193 - BWI76_RS23525 0.73 +0.7 4,908,522 + +1.0 4,908,525 - +0.3 4,908,527 + +0.3 4,908,630 - BWI76_RS23530 0.24 +0.1 4,908,742 - BWI76_RS23530 0.46 +0.8 4,908,880 + BWI76_RS23530 0.73 +1.0 4,908,987 + +0.1 4,909,019 - -1.8 4,909,091 + +1.7 4,909,099 - -1.3 4,909,161 - BWI76_RS23535 0.13 -0.4 4,909,193 - BWI76_RS23535 0.20 +0.5 4,909,247 - BWI76_RS23535 0.32 +0.6 4,909,692 + +0.1 4,909,705 - -0.1 4,909,705 - -0.1 4,909,705 - +1.2 4,909,783 + BWI76_RS23540 0.12 -0.4 4,910,010 - BWI76_RS23540 0.49 +1.0 4,910,046 - BWI76_RS23540 0.55 +0.4 4,910,130 + BWI76_RS23540 0.68 +0.3 4,910,170 - BWI76_RS23540 0.75 +2.2 4,910,187 + BWI76_RS23540 0.78 +0.0 4,910,262 + -2.1 4,910,291 + +0.2 4,910,301 - +0.6 4,910,313 + -0.2 4,910,313 + -0.2 4,910,328 + -0.3 4,910,328 + +0.6 4,910,329 + +0.7 4,910,329 + -0.3 4,910,329 + -0.4 4,910,329 + -0.7 4,910,329 + +0.1 4,910,329 + -0.0 4,910,329 + -0.7 4,910,329 + -0.3 4,910,336 - +1.0 4,910,336 - -0.2 4,910,336 - -1.4 4,910,337 - +1.0 4,910,337 - -0.2 4,910,337 - -1.6 4,910,348 + +0.3 4,910,353 + +1.0 4,910,353 + +1.0 4,910,356 - +0.6 4,910,361 - -0.6 4,910,361 - +0.2 4,910,361 - +0.1 4,910,361 - -1.3 4,910,361 - +0.7 4,910,361 - +0.1 4,910,361 - +0.1 4,910,417 - +0.0 4,910,417 - -0.1 4,910,417 - -1.8 4,910,417 - -0.2 4,910,417 - -0.6 4,910,417 - +0.1 4,910,421 + +1.0 4,910,429 - +1.0 4,910,429 - -0.2 4,910,429 - -2.8 4,910,433 - -0.5 4,910,439 - +0.7 4,910,445 + -0.1 4,910,445 + -0.4 4,910,450 - -0.5 4,910,453 + -1.5 4,910,458 - +0.0 4,910,488 + -1.0 4,910,488 + +1.6 4,910,495 - +2.0 4,910,496 - -0.1 4,910,498 + -1.0 4,910,516 - -0.6 4,910,516 - -1.0 4,910,516 - -0.6 4,910,521 - +1.5 4,910,521 - +2.3 4,910,521 - +0.3 4,910,521 - +0.0 4,910,541 - +0.3
Or see this region's nucleotide sequence