Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23510 and BWI76_RS23515 are separated by 39 nucleotides BWI76_RS23515 and BWI76_RS23520 are separated by 382 nucleotides BWI76_RS23520 and BWI76_RS23525 are separated by 394 nucleotides
BWI76_RS23510: BWI76_RS23510 - transcriptional regulator, at 4,905,473 to 4,906,195
_RS23510
BWI76_RS23515: BWI76_RS23515 - antibiotic biosynthesis monooxygenase, at 4,906,235 to 4,906,552
_RS23515
BWI76_RS23520: BWI76_RS23520 - hypothetical protein, at 4,906,935 to 4,907,144
_RS23520
BWI76_RS23525: BWI76_RS23525 - EamA family transporter, at 4,907,539 to 4,908,429
_RS23525
Position (kb) 4906
4907
4908 Strain fitness (log2 ratio) -1
0
1
2 at 4906.055 kb on - strand, within BWI76_RS23510 at 4906.413 kb on + strand, within BWI76_RS23515 at 4906.488 kb on - strand, within BWI76_RS23515 at 4906.523 kb on + strand at 4906.538 kb on - strand at 4906.612 kb on - strand at 4906.802 kb on + strand at 4907.013 kb on - strand, within BWI76_RS23520 at 4907.133 kb on - strand at 4907.173 kb on - strand at 4907.173 kb on - strand at 4907.380 kb on - strand at 4907.380 kb on - strand at 4907.394 kb on - strand at 4907.407 kb on + strand at 4907.422 kb on - strand at 4907.439 kb on - strand at 4907.526 kb on + strand at 4907.534 kb on - strand at 4907.597 kb on + strand at 4907.753 kb on + strand, within BWI76_RS23525 at 4907.792 kb on - strand, within BWI76_RS23525 at 4907.792 kb on - strand, within BWI76_RS23525 at 4907.796 kb on - strand, within BWI76_RS23525 at 4907.819 kb on - strand, within BWI76_RS23525 at 4907.999 kb on - strand, within BWI76_RS23525 at 4908.128 kb on + strand, within BWI76_RS23525
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 4,906,055 - BWI76_RS23510 0.80 -1.6 4,906,413 + BWI76_RS23515 0.56 -0.6 4,906,488 - BWI76_RS23515 0.80 -0.3 4,906,523 + +0.0 4,906,538 - -1.0 4,906,612 - -0.2 4,906,802 + +0.0 4,907,013 - BWI76_RS23520 0.37 +0.8 4,907,133 - -1.2 4,907,173 - +0.2 4,907,173 - +0.3 4,907,380 - -0.4 4,907,380 - -0.0 4,907,394 - +2.6 4,907,407 + -1.3 4,907,422 - +0.4 4,907,439 - +0.4 4,907,526 + -1.3 4,907,534 - -0.1 4,907,597 + +0.1 4,907,753 + BWI76_RS23525 0.24 +0.0 4,907,792 - BWI76_RS23525 0.28 +0.2 4,907,792 - BWI76_RS23525 0.28 +0.1 4,907,796 - BWI76_RS23525 0.29 -0.5 4,907,819 - BWI76_RS23525 0.31 +1.3 4,907,999 - BWI76_RS23525 0.52 +1.2 4,908,128 + BWI76_RS23525 0.66 -0.3
Or see this region's nucleotide sequence