Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16590

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16585 and BWI76_RS16590 overlap by 4 nucleotidesBWI76_RS16590 and BWI76_RS16595 are separated by 24 nucleotidesBWI76_RS16595 and BWI76_RS16600 are separated by 12 nucleotides BWI76_RS16585: BWI76_RS16585 - trehalose synthase, at 3,435,057 to 3,436,682 _RS16585 BWI76_RS16590: BWI76_RS16590 - short-chain dehydrogenase, at 3,436,679 to 3,437,668 _RS16590 BWI76_RS16595: BWI76_RS16595 - hypothetical protein, at 3,437,693 to 3,438,211 _RS16595 BWI76_RS16600: BWI76_RS16600 - DUF421 domain-containing protein, at 3,438,224 to 3,438,670 _RS16600 Position (kb) 3436 3437 3438Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3435.757 kb on - strand, within BWI76_RS16585at 3435.930 kb on + strand, within BWI76_RS16585at 3436.004 kb on - strand, within BWI76_RS16585at 3436.112 kb on + strand, within BWI76_RS16585at 3436.565 kb on - strandat 3436.971 kb on - strand, within BWI76_RS16590at 3437.313 kb on + strand, within BWI76_RS16590at 3437.617 kb on - strandat 3437.879 kb on - strand, within BWI76_RS16595at 3437.913 kb on + strand, within BWI76_RS16595at 3437.931 kb on + strand, within BWI76_RS16595at 3438.022 kb on + strand, within BWI76_RS16595at 3438.022 kb on + strand, within BWI76_RS16595at 3438.091 kb on + strand, within BWI76_RS16595at 3438.091 kb on + strand, within BWI76_RS16595at 3438.099 kb on - strand, within BWI76_RS16595at 3438.110 kb on - strand, within BWI76_RS16595at 3438.191 kb on + strandat 3438.198 kb on - strandat 3438.212 kb on + strandat 3438.256 kb on - strandat 3438.266 kb on - strandat 3438.266 kb on - strandat 3438.302 kb on + strand, within BWI76_RS16600at 3438.313 kb on + strand, within BWI76_RS16600at 3438.349 kb on - strand, within BWI76_RS16600at 3438.393 kb on + strand, within BWI76_RS16600at 3438.400 kb on - strand, within BWI76_RS16600at 3438.474 kb on + strand, within BWI76_RS16600at 3438.493 kb on + strand, within BWI76_RS16600at 3438.493 kb on + strand, within BWI76_RS16600at 3438.493 kb on + strand, within BWI76_RS16600at 3438.493 kb on + strand, within BWI76_RS16600at 3438.494 kb on + strand, within BWI76_RS16600at 3438.501 kb on - strand, within BWI76_RS16600at 3438.536 kb on + strand, within BWI76_RS16600at 3438.571 kb on + strandat 3438.571 kb on + strand, within BWI76_RS16600at 3438.571 kb on + strand, within BWI76_RS16600at 3438.571 kb on + strand, within BWI76_RS16600at 3438.571 kb on + strand, within BWI76_RS16600at 3438.578 kb on + strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.579 kb on - strand, within BWI76_RS16600at 3438.593 kb on - strand, within BWI76_RS16600at 3438.668 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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3,435,757 - BWI76_RS16585 0.43 +0.6
3,435,930 + BWI76_RS16585 0.54 +0.5
3,436,004 - BWI76_RS16585 0.58 -1.0
3,436,112 + BWI76_RS16585 0.65 +0.2
3,436,565 - +0.8
3,436,971 - BWI76_RS16590 0.29 +0.7
3,437,313 + BWI76_RS16590 0.64 +0.7
3,437,617 - -0.2
3,437,879 - BWI76_RS16595 0.36 -0.3
3,437,913 + BWI76_RS16595 0.42 -0.2
3,437,931 + BWI76_RS16595 0.46 -1.4
3,438,022 + BWI76_RS16595 0.63 -0.5
3,438,022 + BWI76_RS16595 0.63 -1.4
3,438,091 + BWI76_RS16595 0.77 -1.4
3,438,091 + BWI76_RS16595 0.77 +1.0
3,438,099 - BWI76_RS16595 0.78 -0.4
3,438,110 - BWI76_RS16595 0.80 +3.2
3,438,191 + +0.7
3,438,198 - +1.3
3,438,212 + -0.2
3,438,256 - +0.5
3,438,266 - -0.7
3,438,266 - -0.7
3,438,302 + BWI76_RS16600 0.17 -1.7
3,438,313 + BWI76_RS16600 0.20 -1.9
3,438,349 - BWI76_RS16600 0.28 -0.5
3,438,393 + BWI76_RS16600 0.38 +2.3
3,438,400 - BWI76_RS16600 0.39 +0.6
3,438,474 + BWI76_RS16600 0.56 -0.8
3,438,493 + BWI76_RS16600 0.60 +1.9
3,438,493 + BWI76_RS16600 0.60 -0.3
3,438,493 + BWI76_RS16600 0.60 -0.1
3,438,493 + BWI76_RS16600 0.60 -0.6
3,438,494 + BWI76_RS16600 0.60 +0.3
3,438,501 - BWI76_RS16600 0.62 -0.5
3,438,536 + BWI76_RS16600 0.70 +0.9
3,438,571 + +2.2
3,438,571 + BWI76_RS16600 0.78 -1.1
3,438,571 + BWI76_RS16600 0.78 +0.9
3,438,571 + BWI76_RS16600 0.78 -1.1
3,438,571 + BWI76_RS16600 0.78 +1.8
3,438,578 + BWI76_RS16600 0.79 -0.4
3,438,579 - BWI76_RS16600 0.79 +0.9
3,438,579 - BWI76_RS16600 0.79 -0.1
3,438,579 - BWI76_RS16600 0.79 -0.8
3,438,579 - BWI76_RS16600 0.79 +1.1
3,438,579 - BWI76_RS16600 0.79 -0.7
3,438,579 - BWI76_RS16600 0.79 +0.9
3,438,579 - BWI76_RS16600 0.79 -0.4
3,438,579 - BWI76_RS16600 0.79 -0.4
3,438,579 - BWI76_RS16600 0.79 +0.7
3,438,579 - BWI76_RS16600 0.79 +0.5
3,438,579 - BWI76_RS16600 0.79 -1.0
3,438,579 - BWI76_RS16600 0.79 -0.2
3,438,593 - BWI76_RS16600 0.83 -0.8
3,438,668 - +0.9

Or see this region's nucleotide sequence