Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14945 and BWI76_RS14950 are separated by 4 nucleotides BWI76_RS14950 and BWI76_RS14955 are separated by 3 nucleotides BWI76_RS14955 and BWI76_RS14960 are separated by 217 nucleotides
BWI76_RS14945: BWI76_RS14945 - PTS system, cellobiose-specific IIC component, at 3,131,958 to 3,133,277
_RS14945
BWI76_RS14950: BWI76_RS14950 - glycosylasparaginase, at 3,133,282 to 3,134,235
_RS14950
BWI76_RS14955: BWI76_RS14955 - peptidase M17 leucyl aminopeptidase domain-containing protein, at 3,134,239 to 3,135,594
_RS14955
BWI76_RS14960: BWI76_RS14960 - MFS transporter, at 3,135,812 to 3,137,011
_RS14960
Position (kb) 3134
3135
3136 Strain fitness (log2 ratio) -1
0
1 at 3133.370 kb on + strand at 3133.421 kb on - strand at 3133.637 kb on + strand, within BWI76_RS14950 at 3133.851 kb on - strand, within BWI76_RS14950 at 3133.851 kb on - strand, within BWI76_RS14950 at 3134.204 kb on - strand at 3134.232 kb on - strand at 3134.529 kb on - strand, within BWI76_RS14955 at 3134.561 kb on + strand, within BWI76_RS14955 at 3135.083 kb on + strand, within BWI76_RS14955 at 3135.123 kb on + strand, within BWI76_RS14955 at 3135.314 kb on + strand, within BWI76_RS14955 at 3135.319 kb on + strand, within BWI76_RS14955 at 3135.346 kb on + strand, within BWI76_RS14955 at 3135.393 kb on + strand, within BWI76_RS14955 at 3135.632 kb on - strand at 3135.733 kb on - strand at 3136.243 kb on + strand, within BWI76_RS14960 at 3136.251 kb on - strand, within BWI76_RS14960 at 3136.251 kb on - strand, within BWI76_RS14960 at 3136.289 kb on - strand, within BWI76_RS14960 at 3136.381 kb on - strand, within BWI76_RS14960 at 3136.427 kb on + strand, within BWI76_RS14960 at 3136.434 kb on + strand, within BWI76_RS14960
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,133,370 + -0.6 3,133,421 - +0.0 3,133,637 + BWI76_RS14950 0.37 +1.0 3,133,851 - BWI76_RS14950 0.60 +0.3 3,133,851 - BWI76_RS14950 0.60 +0.6 3,134,204 - -0.1 3,134,232 - +1.3 3,134,529 - BWI76_RS14955 0.21 +1.3 3,134,561 + BWI76_RS14955 0.24 +1.0 3,135,083 + BWI76_RS14955 0.62 -0.1 3,135,123 + BWI76_RS14955 0.65 +1.0 3,135,314 + BWI76_RS14955 0.79 +1.0 3,135,319 + BWI76_RS14955 0.80 +0.6 3,135,346 + BWI76_RS14955 0.82 +1.2 3,135,393 + BWI76_RS14955 0.85 -0.8 3,135,632 - +0.5 3,135,733 - -0.7 3,136,243 + BWI76_RS14960 0.36 +0.3 3,136,251 - BWI76_RS14960 0.37 -0.0 3,136,251 - BWI76_RS14960 0.37 -1.0 3,136,289 - BWI76_RS14960 0.40 +0.6 3,136,381 - BWI76_RS14960 0.47 -1.1 3,136,427 + BWI76_RS14960 0.51 -0.2 3,136,434 + BWI76_RS14960 0.52 -0.2
Or see this region's nucleotide sequence