Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14555 and BWI76_RS14560 are separated by 161 nucleotides BWI76_RS14560 and BWI76_RS14565 are separated by 476 nucleotides
BWI76_RS14555: BWI76_RS14555 - Yersiniabactin/pesticin outer membrane receptor (IRPC), at 3,055,284 to 3,057,305
_RS14555
BWI76_RS14560: BWI76_RS14560 - peptidoglycan-binding protein LysM, at 3,057,467 to 3,057,916
_RS14560
BWI76_RS14565: BWI76_RS14565 - cysteine hydrolase, at 3,058,393 to 3,058,926
_RS14565
Position (kb)
3057
3058 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 3056.622 kb on + strand, within BWI76_RS14555 at 3056.645 kb on - strand, within BWI76_RS14555 at 3056.652 kb on - strand, within BWI76_RS14555 at 3056.665 kb on + strand, within BWI76_RS14555 at 3056.809 kb on + strand, within BWI76_RS14555 at 3056.812 kb on - strand, within BWI76_RS14555 at 3056.817 kb on - strand, within BWI76_RS14555 at 3056.850 kb on - strand, within BWI76_RS14555 at 3056.958 kb on + strand, within BWI76_RS14555 at 3057.048 kb on + strand, within BWI76_RS14555 at 3057.137 kb on - strand at 3057.137 kb on - strand at 3057.205 kb on - strand at 3057.254 kb on + strand at 3057.254 kb on + strand at 3057.262 kb on - strand at 3057.262 kb on - strand at 3057.262 kb on - strand at 3057.262 kb on - strand at 3057.373 kb on - strand at 3057.386 kb on - strand at 3057.580 kb on - strand, within BWI76_RS14560 at 3057.652 kb on + strand, within BWI76_RS14560 at 3057.652 kb on + strand, within BWI76_RS14560 at 3057.652 kb on + strand, within BWI76_RS14560 at 3057.662 kb on + strand, within BWI76_RS14560 at 3057.662 kb on + strand, within BWI76_RS14560 at 3057.662 kb on + strand, within BWI76_RS14560 at 3057.662 kb on + strand, within BWI76_RS14560 at 3057.670 kb on - strand, within BWI76_RS14560 at 3057.671 kb on - strand, within BWI76_RS14560 at 3057.671 kb on - strand, within BWI76_RS14560 at 3057.671 kb on - strand, within BWI76_RS14560 at 3057.673 kb on + strand, within BWI76_RS14560 at 3057.681 kb on - strand, within BWI76_RS14560 at 3057.740 kb on + strand, within BWI76_RS14560 at 3057.749 kb on - strand, within BWI76_RS14560 at 3057.776 kb on + strand, within BWI76_RS14560 at 3057.802 kb on + strand, within BWI76_RS14560 at 3057.805 kb on - strand, within BWI76_RS14560 at 3057.841 kb on + strand, within BWI76_RS14560 at 3058.017 kb on + strand at 3058.022 kb on + strand at 3058.025 kb on - strand at 3058.025 kb on - strand at 3058.034 kb on - strand at 3058.071 kb on + strand at 3058.117 kb on - strand at 3058.248 kb on - strand at 3058.257 kb on - strand at 3058.266 kb on + strand at 3058.282 kb on + strand at 3058.318 kb on + strand at 3058.353 kb on - strand at 3058.387 kb on + strand at 3058.596 kb on - strand, within BWI76_RS14565
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 3,056,622 + BWI76_RS14555 0.66 +2.0 3,056,645 - BWI76_RS14555 0.67 -0.1 3,056,652 - BWI76_RS14555 0.68 +0.1 3,056,665 + BWI76_RS14555 0.68 -1.9 3,056,809 + BWI76_RS14555 0.75 +0.5 3,056,812 - BWI76_RS14555 0.76 -0.6 3,056,817 - BWI76_RS14555 0.76 +0.4 3,056,850 - BWI76_RS14555 0.77 -0.0 3,056,958 + BWI76_RS14555 0.83 +0.1 3,057,048 + BWI76_RS14555 0.87 +0.6 3,057,137 - +0.8 3,057,137 - -0.7 3,057,205 - -0.2 3,057,254 + +0.0 3,057,254 + -0.1 3,057,262 - -0.6 3,057,262 - +0.1 3,057,262 - -2.0 3,057,262 - -0.1 3,057,373 - -2.4 3,057,386 - -0.0 3,057,580 - BWI76_RS14560 0.25 +0.5 3,057,652 + BWI76_RS14560 0.41 +0.4 3,057,652 + BWI76_RS14560 0.41 +0.3 3,057,652 + BWI76_RS14560 0.41 +0.0 3,057,662 + BWI76_RS14560 0.43 +1.3 3,057,662 + BWI76_RS14560 0.43 +0.4 3,057,662 + BWI76_RS14560 0.43 -0.1 3,057,662 + BWI76_RS14560 0.43 +0.2 3,057,670 - BWI76_RS14560 0.45 +0.9 3,057,671 - BWI76_RS14560 0.45 +0.4 3,057,671 - BWI76_RS14560 0.45 +0.9 3,057,671 - BWI76_RS14560 0.45 -0.6 3,057,673 + BWI76_RS14560 0.46 -0.3 3,057,681 - BWI76_RS14560 0.48 -0.1 3,057,740 + BWI76_RS14560 0.61 -0.1 3,057,749 - BWI76_RS14560 0.63 +0.5 3,057,776 + BWI76_RS14560 0.69 +0.5 3,057,802 + BWI76_RS14560 0.74 -0.1 3,057,805 - BWI76_RS14560 0.75 +2.3 3,057,841 + BWI76_RS14560 0.83 -2.5 3,058,017 + -0.3 3,058,022 + +0.1 3,058,025 - +0.8 3,058,025 - +0.2 3,058,034 - +0.3 3,058,071 + +0.3 3,058,117 - +0.7 3,058,248 - -0.1 3,058,257 - -1.0 3,058,266 + +0.7 3,058,282 + -0.2 3,058,318 + +0.1 3,058,353 - -1.5 3,058,387 + -0.6 3,058,596 - BWI76_RS14565 0.38 -0.8
Or see this region's nucleotide sequence