Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11620 and BWI76_RS11625 are separated by 86 nucleotides BWI76_RS11625 and BWI76_RS11630 are separated by 140 nucleotides BWI76_RS11630 and BWI76_RS11635 are separated by 78 nucleotides BWI76_RS11635 and BWI76_RS11640 overlap by 1 nucleotides
BWI76_RS11620: BWI76_RS11620 - pyrimidine (deoxy)nucleoside triphosphate pyrophosphohydrolase, at 2,445,578 to 2,445,988
_RS11620
BWI76_RS11625: BWI76_RS11625 - membrane protein, at 2,446,075 to 2,446,692
_RS11625
BWI76_RS11630: BWI76_RS11630 - sulfurtransferase, at 2,446,833 to 2,448,140
_RS11630
BWI76_RS11635: BWI76_RS11635 - ABC transporter, at 2,448,219 to 2,448,851
_RS11635
BWI76_RS11640: BWI76_RS11640 - ABC transporter membrane protein, at 2,448,851 to 2,450,386
_RS11640
Position (kb)
2446
2447
2448
2449 Strain fitness (log2 ratio)
-1
0
1 at 2446.039 kb on - strand at 2446.640 kb on + strand at 2446.658 kb on - strand at 2446.688 kb on - strand at 2446.745 kb on - strand at 2447.274 kb on - strand, within BWI76_RS11630 at 2447.385 kb on + strand, within BWI76_RS11630 at 2447.503 kb on + strand, within BWI76_RS11630 at 2447.756 kb on - strand, within BWI76_RS11630 at 2447.864 kb on - strand, within BWI76_RS11630 at 2447.902 kb on - strand, within BWI76_RS11630 at 2448.372 kb on - strand, within BWI76_RS11635 at 2448.574 kb on + strand, within BWI76_RS11635 at 2448.798 kb on + strand at 2448.862 kb on - strand at 2448.929 kb on - strand at 2448.964 kb on - strand at 2449.006 kb on - strand, within BWI76_RS11640
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,446,039 - +0.4 2,446,640 + +1.7 2,446,658 - +0.1 2,446,688 - -0.2 2,446,745 - -0.7 2,447,274 - BWI76_RS11630 0.34 -0.9 2,447,385 + BWI76_RS11630 0.42 -0.4 2,447,503 + BWI76_RS11630 0.51 +0.4 2,447,756 - BWI76_RS11630 0.71 +0.7 2,447,864 - BWI76_RS11630 0.79 +1.1 2,447,902 - BWI76_RS11630 0.82 +1.4 2,448,372 - BWI76_RS11635 0.24 +0.3 2,448,574 + BWI76_RS11635 0.56 -1.3 2,448,798 + -1.4 2,448,862 - +0.2 2,448,929 - +0.7 2,448,964 - -0.4 2,449,006 - BWI76_RS11640 0.10 +0.5
Or see this region's nucleotide sequence