Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10410 and BWI76_RS10415 are separated by 91 nucleotides BWI76_RS10415 and BWI76_RS10420 are separated by 190 nucleotides BWI76_RS10420 and BWI76_RS10425 are separated by 43 nucleotides BWI76_RS10425 and BWI76_RS10430 are separated by 107 nucleotides
BWI76_RS10410: BWI76_RS10410 - hypothetical protein, at 2,202,442 to 2,202,702
_RS10410
BWI76_RS10415: BWI76_RS10415 - endoribonuclease L-PSP, at 2,202,794 to 2,203,171
_RS10415
BWI76_RS10420: BWI76_RS10420 - monooxygenase, at 2,203,362 to 2,204,390
_RS10420
BWI76_RS10425: BWI76_RS10425 - AIG2 family protein, at 2,204,434 to 2,204,769
_RS10425
BWI76_RS10430: BWI76_RS10430 - thiol:disulfide interchange protein DsbG, at 2,204,877 to 2,205,635
_RS10430
Position (kb)
2203
2204
2205 Strain fitness (log2 ratio)
-1
0
1 at 2202.389 kb on + strand at 2202.394 kb on + strand at 2202.394 kb on + strand at 2202.442 kb on - strand at 2202.498 kb on - strand, within BWI76_RS10410 at 2202.610 kb on - strand, within BWI76_RS10410 at 2202.806 kb on - strand at 2202.835 kb on + strand, within BWI76_RS10415 at 2202.845 kb on + strand, within BWI76_RS10415 at 2202.979 kb on + strand, within BWI76_RS10415 at 2202.979 kb on + strand, within BWI76_RS10415 at 2203.222 kb on - strand at 2203.281 kb on + strand at 2203.281 kb on + strand at 2203.289 kb on - strand at 2203.289 kb on - strand at 2203.625 kb on + strand, within BWI76_RS10420 at 2203.633 kb on - strand, within BWI76_RS10420 at 2203.633 kb on - strand, within BWI76_RS10420 at 2203.672 kb on + strand, within BWI76_RS10420 at 2203.729 kb on + strand, within BWI76_RS10420 at 2203.880 kb on + strand, within BWI76_RS10420 at 2204.239 kb on - strand, within BWI76_RS10420 at 2204.329 kb on - strand at 2204.744 kb on - strand at 2205.247 kb on + strand, within BWI76_RS10430 at 2205.360 kb on + strand, within BWI76_RS10430
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 2,202,389 + +0.1 2,202,394 + +0.1 2,202,394 + -1.0 2,202,442 - -0.2 2,202,498 - BWI76_RS10410 0.21 +1.0 2,202,610 - BWI76_RS10410 0.64 -1.0 2,202,806 - +0.3 2,202,835 + BWI76_RS10415 0.11 -0.6 2,202,845 + BWI76_RS10415 0.13 -0.6 2,202,979 + BWI76_RS10415 0.49 +0.5 2,202,979 + BWI76_RS10415 0.49 -0.5 2,203,222 - +1.0 2,203,281 + -1.5 2,203,281 + -1.3 2,203,289 - +1.0 2,203,289 - -0.8 2,203,625 + BWI76_RS10420 0.26 +0.0 2,203,633 - BWI76_RS10420 0.26 -0.0 2,203,633 - BWI76_RS10420 0.26 +0.8 2,203,672 + BWI76_RS10420 0.30 +0.8 2,203,729 + BWI76_RS10420 0.36 +0.7 2,203,880 + BWI76_RS10420 0.50 +0.7 2,204,239 - BWI76_RS10420 0.85 +0.3 2,204,329 - -1.0 2,204,744 - +0.0 2,205,247 + BWI76_RS10430 0.49 +0.3 2,205,360 + BWI76_RS10430 0.64 -1.3
Or see this region's nucleotide sequence