Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS05670

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS05665 and BWI76_RS05670 are separated by 103 nucleotidesBWI76_RS05670 and BWI76_RS05675 are separated by 123 nucleotides BWI76_RS05665: BWI76_RS05665 - SAM-dependent methyltransferase, at 1,189,000 to 1,190,628 _RS05665 BWI76_RS05670: BWI76_RS05670 - porin, at 1,190,732 to 1,192,072 _RS05670 BWI76_RS05675: BWI76_RS05675 - MFS transporter, at 1,192,196 to 1,193,593 _RS05675 Position (kb) 1190 1191 1192 1193Strain fitness (log2 ratio) -3 -2 -1 0 1at 1189.754 kb on + strand, within BWI76_RS05665at 1189.968 kb on - strand, within BWI76_RS05665at 1189.999 kb on - strand, within BWI76_RS05665at 1190.044 kb on - strand, within BWI76_RS05665at 1190.044 kb on - strand, within BWI76_RS05665at 1190.254 kb on - strand, within BWI76_RS05665at 1190.480 kb on + strandat 1190.654 kb on - strandat 1190.815 kb on + strandat 1190.835 kb on - strandat 1190.853 kb on + strandat 1190.853 kb on + strandat 1190.853 kb on + strandat 1190.945 kb on - strand, within BWI76_RS05670at 1190.999 kb on + strand, within BWI76_RS05670at 1191.166 kb on - strand, within BWI76_RS05670at 1191.264 kb on + strand, within BWI76_RS05670at 1191.481 kb on + strand, within BWI76_RS05670at 1191.500 kb on - strand, within BWI76_RS05670at 1191.603 kb on - strand, within BWI76_RS05670at 1191.675 kb on - strand, within BWI76_RS05670at 1191.836 kb on - strand, within BWI76_RS05670at 1191.855 kb on + strand, within BWI76_RS05670at 1191.947 kb on + strandat 1192.016 kb on + strandat 1192.023 kb on + strandat 1192.067 kb on + strandat 1192.111 kb on + strandat 1192.184 kb on + strandat 1192.329 kb on - strandat 1192.345 kb on + strand, within BWI76_RS05675at 1192.366 kb on + strand, within BWI76_RS05675at 1192.374 kb on - strand, within BWI76_RS05675at 1192.379 kb on - strand, within BWI76_RS05675at 1192.388 kb on + strand, within BWI76_RS05675at 1192.451 kb on + strand, within BWI76_RS05675at 1192.468 kb on + strand, within BWI76_RS05675at 1192.469 kb on + strand, within BWI76_RS05675at 1192.632 kb on - strand, within BWI76_RS05675at 1192.646 kb on + strand, within BWI76_RS05675at 1192.660 kb on - strand, within BWI76_RS05675at 1192.741 kb on - strand, within BWI76_RS05675at 1192.759 kb on + strand, within BWI76_RS05675at 1192.759 kb on + strand, within BWI76_RS05675at 1192.759 kb on + strand, within BWI76_RS05675at 1192.798 kb on + strand, within BWI76_RS05675at 1192.805 kb on - strand, within BWI76_RS05675at 1192.820 kb on + strand, within BWI76_RS05675at 1192.861 kb on - strand, within BWI76_RS05675at 1192.979 kb on + strand, within BWI76_RS05675at 1192.980 kb on - strand, within BWI76_RS05675

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
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1,189,754 + BWI76_RS05665 0.46 -2.8
1,189,968 - BWI76_RS05665 0.59 -0.9
1,189,999 - BWI76_RS05665 0.61 +0.3
1,190,044 - BWI76_RS05665 0.64 +0.6
1,190,044 - BWI76_RS05665 0.64 +1.0
1,190,254 - BWI76_RS05665 0.77 +0.6
1,190,480 + +0.6
1,190,654 - +0.8
1,190,815 + +1.1
1,190,835 - +0.4
1,190,853 + -0.3
1,190,853 + -2.9
1,190,853 + +1.1
1,190,945 - BWI76_RS05670 0.16 +1.1
1,190,999 + BWI76_RS05670 0.20 +0.1
1,191,166 - BWI76_RS05670 0.32 -0.9
1,191,264 + BWI76_RS05670 0.40 +0.2
1,191,481 + BWI76_RS05670 0.56 -0.1
1,191,500 - BWI76_RS05670 0.57 -0.4
1,191,603 - BWI76_RS05670 0.65 -0.3
1,191,675 - BWI76_RS05670 0.70 -0.1
1,191,836 - BWI76_RS05670 0.82 +0.5
1,191,855 + BWI76_RS05670 0.84 +1.4
1,191,947 + -0.5
1,192,016 + -0.7
1,192,023 + +1.1
1,192,067 + -0.4
1,192,111 + -0.2
1,192,184 + +0.1
1,192,329 - -0.2
1,192,345 + BWI76_RS05675 0.11 -2.4
1,192,366 + BWI76_RS05675 0.12 +0.0
1,192,374 - BWI76_RS05675 0.13 +1.1
1,192,379 - BWI76_RS05675 0.13 -0.1
1,192,388 + BWI76_RS05675 0.14 +0.1
1,192,451 + BWI76_RS05675 0.18 -2.5
1,192,468 + BWI76_RS05675 0.19 -0.4
1,192,469 + BWI76_RS05675 0.20 -0.5
1,192,632 - BWI76_RS05675 0.31 +0.2
1,192,646 + BWI76_RS05675 0.32 +0.2
1,192,660 - BWI76_RS05675 0.33 -0.5
1,192,741 - BWI76_RS05675 0.39 +0.0
1,192,759 + BWI76_RS05675 0.40 +0.1
1,192,759 + BWI76_RS05675 0.40 -0.5
1,192,759 + BWI76_RS05675 0.40 +0.1
1,192,798 + BWI76_RS05675 0.43 +0.8
1,192,805 - BWI76_RS05675 0.44 +1.3
1,192,820 + BWI76_RS05675 0.45 -0.5
1,192,861 - BWI76_RS05675 0.48 -2.4
1,192,979 + BWI76_RS05675 0.56 +0.3
1,192,980 - BWI76_RS05675 0.56 +0.5

Or see this region's nucleotide sequence