Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05650 and BWI76_RS05655 are separated by 583 nucleotides BWI76_RS05655 and BWI76_RS05660 are separated by 10 nucleotides BWI76_RS05660 and BWI76_RS05665 are separated by 17 nucleotides
BWI76_RS05650: BWI76_RS05650 - mannonate dehydratase, at 1,186,395 to 1,187,579
_RS05650
BWI76_RS05655: BWI76_RS05655 - hypothetical protein, at 1,188,163 to 1,188,420
_RS05655
BWI76_RS05660: BWI76_RS05660 - N-acetyltransferase, at 1,188,431 to 1,188,982
_RS05660
BWI76_RS05665: BWI76_RS05665 - SAM-dependent methyltransferase, at 1,189,000 to 1,190,628
_RS05665
Position (kb)
1188
1189 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1187.552 kb on + strand at 1187.560 kb on - strand at 1187.606 kb on + strand at 1187.669 kb on - strand at 1187.778 kb on - strand at 1187.824 kb on + strand at 1187.837 kb on + strand at 1187.837 kb on + strand at 1187.845 kb on - strand at 1187.845 kb on - strand at 1187.845 kb on - strand at 1187.845 kb on - strand at 1188.010 kb on + strand at 1188.204 kb on + strand, within BWI76_RS05655 at 1188.368 kb on - strand, within BWI76_RS05655 at 1188.377 kb on - strand, within BWI76_RS05655 at 1188.494 kb on + strand, within BWI76_RS05660 at 1188.494 kb on + strand, within BWI76_RS05660 at 1188.637 kb on + strand, within BWI76_RS05660 at 1188.680 kb on - strand, within BWI76_RS05660 at 1188.680 kb on - strand, within BWI76_RS05660 at 1188.725 kb on + strand, within BWI76_RS05660 at 1188.741 kb on + strand, within BWI76_RS05660 at 1188.741 kb on + strand, within BWI76_RS05660 at 1188.751 kb on + strand, within BWI76_RS05660 at 1188.785 kb on - strand, within BWI76_RS05660 at 1188.816 kb on + strand, within BWI76_RS05660 at 1188.824 kb on - strand, within BWI76_RS05660 at 1188.844 kb on - strand, within BWI76_RS05660 at 1188.853 kb on + strand, within BWI76_RS05660 at 1188.955 kb on + strand at 1189.046 kb on + strand at 1189.360 kb on - strand, within BWI76_RS05665 at 1189.504 kb on + strand, within BWI76_RS05665 at 1189.512 kb on - strand, within BWI76_RS05665 at 1189.585 kb on + strand, within BWI76_RS05665 at 1189.619 kb on - strand, within BWI76_RS05665 at 1189.647 kb on + strand, within BWI76_RS05665 at 1189.754 kb on + strand, within BWI76_RS05665 at 1189.968 kb on - strand, within BWI76_RS05665
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 1,187,552 + -0.2 1,187,560 - +0.4 1,187,606 + -0.9 1,187,669 - -0.4 1,187,778 - +0.8 1,187,824 + +1.1 1,187,837 + -0.2 1,187,837 + -2.2 1,187,845 - +0.1 1,187,845 - +0.1 1,187,845 - +0.2 1,187,845 - -1.6 1,188,010 + -0.4 1,188,204 + BWI76_RS05655 0.16 -1.1 1,188,368 - BWI76_RS05655 0.79 -0.5 1,188,377 - BWI76_RS05655 0.83 -1.5 1,188,494 + BWI76_RS05660 0.11 -0.5 1,188,494 + BWI76_RS05660 0.11 -0.6 1,188,637 + BWI76_RS05660 0.37 +0.1 1,188,680 - BWI76_RS05660 0.45 -0.4 1,188,680 - BWI76_RS05660 0.45 -0.6 1,188,725 + BWI76_RS05660 0.53 +0.3 1,188,741 + BWI76_RS05660 0.56 +0.4 1,188,741 + BWI76_RS05660 0.56 -2.4 1,188,751 + BWI76_RS05660 0.58 +0.3 1,188,785 - BWI76_RS05660 0.64 -0.1 1,188,816 + BWI76_RS05660 0.70 -0.5 1,188,824 - BWI76_RS05660 0.71 -0.8 1,188,844 - BWI76_RS05660 0.75 -0.1 1,188,853 + BWI76_RS05660 0.76 -2.1 1,188,955 + +0.1 1,189,046 + -0.3 1,189,360 - BWI76_RS05665 0.22 -0.1 1,189,504 + BWI76_RS05665 0.31 -0.0 1,189,512 - BWI76_RS05665 0.31 +0.1 1,189,585 + BWI76_RS05665 0.36 -1.5 1,189,619 - BWI76_RS05665 0.38 -0.2 1,189,647 + BWI76_RS05665 0.40 -0.6 1,189,754 + BWI76_RS05665 0.46 -2.8 1,189,968 - BWI76_RS05665 0.59 -0.9
Or see this region's nucleotide sequence