Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02085 and BWI76_RS02090 overlap by 4 nucleotides BWI76_RS02090 and BWI76_RS02095 are separated by 182 nucleotides
BWI76_RS02085: BWI76_RS02085 - cation acetate symporter, at 429,691 to 431,340
_RS02085
BWI76_RS02090: BWI76_RS02090 - membrane protein, at 431,337 to 431,651
_RS02090
BWI76_RS02095: BWI76_RS02095 - acetyl-coenzyme A synthetase, at 431,834 to 433,792
_RS02095
Position (kb) 431
432 Strain fitness (log2 ratio) -2
-1
0
1 at 430.405 kb on + strand, within BWI76_RS02085 at 430.481 kb on - strand, within BWI76_RS02085 at 430.525 kb on - strand, within BWI76_RS02085 at 430.538 kb on - strand, within BWI76_RS02085 at 430.673 kb on - strand, within BWI76_RS02085 at 430.696 kb on + strand, within BWI76_RS02085 at 430.745 kb on - strand, within BWI76_RS02085 at 430.888 kb on + strand, within BWI76_RS02085 at 430.914 kb on + strand, within BWI76_RS02085 at 430.922 kb on - strand, within BWI76_RS02085 at 430.976 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.056 kb on - strand, within BWI76_RS02085 at 431.082 kb on - strand, within BWI76_RS02085 at 431.192 kb on + strand at 431.200 kb on - strand at 431.265 kb on - strand at 431.265 kb on - strand at 431.270 kb on - strand at 431.323 kb on + strand at 431.344 kb on + strand at 431.502 kb on - strand, within BWI76_RS02090 at 431.536 kb on - strand, within BWI76_RS02090 at 431.559 kb on - strand, within BWI76_RS02090 at 431.668 kb on - strand at 431.726 kb on - strand at 431.728 kb on + strand at 431.762 kb on + strand at 431.770 kb on - strand at 431.815 kb on + strand at 431.847 kb on + strand at 431.901 kb on + strand at 432.063 kb on + strand, within BWI76_RS02095 at 432.066 kb on - strand at 432.071 kb on - strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.094 kb on + strand, within BWI76_RS02095 at 432.110 kb on + strand, within BWI76_RS02095 at 432.126 kb on + strand, within BWI76_RS02095 at 432.297 kb on + strand, within BWI76_RS02095 at 432.381 kb on + strand, within BWI76_RS02095 at 432.457 kb on + strand, within BWI76_RS02095 at 432.526 kb on + strand, within BWI76_RS02095 at 432.620 kb on - strand, within BWI76_RS02095
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 430,405 + BWI76_RS02085 0.43 +0.5 430,481 - BWI76_RS02085 0.48 -0.2 430,525 - BWI76_RS02085 0.51 +0.7 430,538 - BWI76_RS02085 0.51 -0.3 430,673 - BWI76_RS02085 0.60 +1.6 430,696 + BWI76_RS02085 0.61 +1.3 430,745 - BWI76_RS02085 0.64 +0.4 430,888 + BWI76_RS02085 0.73 +1.0 430,914 + BWI76_RS02085 0.74 +0.4 430,922 - BWI76_RS02085 0.75 +0.4 430,976 - BWI76_RS02085 0.78 +0.3 431,056 - BWI76_RS02085 0.83 -2.1 431,056 - BWI76_RS02085 0.83 -0.3 431,082 - BWI76_RS02085 0.84 -0.8 431,192 + +0.2 431,200 - +0.5 431,265 - -0.7 431,265 - -1.3 431,270 - -0.4 431,323 + +0.4 431,344 + +0.6 431,502 - BWI76_RS02090 0.52 -0.9 431,536 - BWI76_RS02090 0.63 +1.2 431,559 - BWI76_RS02090 0.70 -1.1 431,668 - -0.2 431,726 - +0.0 431,728 + +0.6 431,762 + +0.5 431,770 - +0.5 431,815 + +0.1 431,847 + -0.4 431,901 + +0.6 432,063 + BWI76_RS02095 0.12 -2.5 432,066 - +0.2 432,071 - BWI76_RS02095 0.12 +0.1 432,094 + BWI76_RS02095 0.13 -0.4 432,094 + BWI76_RS02095 0.13 +0.5 432,110 + BWI76_RS02095 0.14 +0.4 432,126 + BWI76_RS02095 0.15 -0.5 432,297 + BWI76_RS02095 0.24 -2.5 432,381 + BWI76_RS02095 0.28 -0.2 432,457 + BWI76_RS02095 0.32 -0.3 432,526 + BWI76_RS02095 0.35 -0.4 432,620 - BWI76_RS02095 0.40 +0.2
Or see this region's nucleotide sequence