Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01820 and BWI76_RS01825 are separated by 14 nucleotides BWI76_RS01825 and BWI76_RS01830 are separated by 187 nucleotides
BWI76_RS01820: BWI76_RS01820 - maltose ABC transporter, permease component 2 (MalG) <small>(from data)</small>, at 370,662 to 371,552
_RS01820
BWI76_RS01825: BWI76_RS01825 - maltose ABC transporter, permease component 1 (MalF) <small>(from data)</small>, at 371,567 to 373,111
_RS01825
BWI76_RS01830: BWI76_RS01830 - maltose ABC transporter, substrate-binding component MalE <small>(from data)</small>, at 373,299 to 374,489
_RS01830
Position (kb) 371
372
373
374 Strain fitness (log2 ratio) -2
-1
0
1
2 at 370.592 kb on + strand at 370.722 kb on + strand at 370.741 kb on + strand at 370.820 kb on + strand, within BWI76_RS01820 at 370.820 kb on + strand, within BWI76_RS01820 at 370.828 kb on - strand, within BWI76_RS01820 at 370.828 kb on - strand, within BWI76_RS01820 at 370.835 kb on - strand, within BWI76_RS01820 at 370.859 kb on + strand, within BWI76_RS01820 at 370.937 kb on - strand, within BWI76_RS01820 at 371.116 kb on - strand, within BWI76_RS01820 at 371.182 kb on + strand, within BWI76_RS01820 at 371.182 kb on + strand, within BWI76_RS01820 at 371.239 kb on + strand, within BWI76_RS01820 at 371.698 kb on + strand at 371.729 kb on - strand, within BWI76_RS01825 at 371.884 kb on + strand, within BWI76_RS01825 at 371.884 kb on + strand, within BWI76_RS01825 at 371.892 kb on - strand, within BWI76_RS01825 at 372.135 kb on + strand, within BWI76_RS01825 at 372.203 kb on + strand, within BWI76_RS01825 at 372.211 kb on - strand, within BWI76_RS01825 at 372.259 kb on - strand, within BWI76_RS01825 at 372.349 kb on + strand, within BWI76_RS01825 at 372.361 kb on + strand, within BWI76_RS01825 at 372.452 kb on + strand, within BWI76_RS01825 at 372.661 kb on + strand, within BWI76_RS01825 at 372.833 kb on - strand, within BWI76_RS01825 at 372.837 kb on + strand at 372.883 kb on + strand, within BWI76_RS01825 at 372.920 kb on + strand, within BWI76_RS01825 at 373.023 kb on + strand at 373.023 kb on + strand at 373.026 kb on - strand at 373.147 kb on + strand at 373.151 kb on + strand at 373.230 kb on - strand at 373.261 kb on + strand at 373.329 kb on - strand at 373.371 kb on - strand at 373.512 kb on + strand, within BWI76_RS01830 at 373.643 kb on + strand, within BWI76_RS01830 at 373.681 kb on + strand, within BWI76_RS01830 at 373.684 kb on - strand, within BWI76_RS01830 at 373.716 kb on - strand, within BWI76_RS01830 at 373.860 kb on + strand, within BWI76_RS01830 at 373.969 kb on - strand, within BWI76_RS01830 at 373.978 kb on + strand, within BWI76_RS01830
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^5 spores/mL remove 370,592 + +0.7 370,722 + +0.2 370,741 + +0.2 370,820 + BWI76_RS01820 0.18 -0.8 370,820 + BWI76_RS01820 0.18 +0.2 370,828 - BWI76_RS01820 0.19 +0.9 370,828 - BWI76_RS01820 0.19 -0.8 370,835 - BWI76_RS01820 0.19 +0.2 370,859 + BWI76_RS01820 0.22 +1.0 370,937 - BWI76_RS01820 0.31 +0.8 371,116 - BWI76_RS01820 0.51 -0.3 371,182 + BWI76_RS01820 0.58 +0.3 371,182 + BWI76_RS01820 0.58 -0.4 371,239 + BWI76_RS01820 0.65 +0.4 371,698 + -0.2 371,729 - BWI76_RS01825 0.10 +0.9 371,884 + BWI76_RS01825 0.21 -1.8 371,884 + BWI76_RS01825 0.21 -0.1 371,892 - BWI76_RS01825 0.21 -0.8 372,135 + BWI76_RS01825 0.37 +1.1 372,203 + BWI76_RS01825 0.41 +1.7 372,211 - BWI76_RS01825 0.42 +0.2 372,259 - BWI76_RS01825 0.45 +0.3 372,349 + BWI76_RS01825 0.51 +0.3 372,361 + BWI76_RS01825 0.51 +1.8 372,452 + BWI76_RS01825 0.57 +1.1 372,661 + BWI76_RS01825 0.71 +0.0 372,833 - BWI76_RS01825 0.82 +0.1 372,837 + +0.2 372,883 + BWI76_RS01825 0.85 -0.3 372,920 + BWI76_RS01825 0.88 +0.6 373,023 + +0.3 373,023 + +1.8 373,026 - -1.3 373,147 + -0.8 373,151 + -0.6 373,230 - +0.1 373,261 + -1.7 373,329 - -2.1 373,371 - +0.8 373,512 + BWI76_RS01830 0.18 -0.3 373,643 + BWI76_RS01830 0.29 +0.4 373,681 + BWI76_RS01830 0.32 -0.0 373,684 - BWI76_RS01830 0.32 -0.1 373,716 - BWI76_RS01830 0.35 +0.6 373,860 + BWI76_RS01830 0.47 -0.9 373,969 - BWI76_RS01830 0.56 +1.1 373,978 + BWI76_RS01830 0.57 -1.0
Or see this region's nucleotide sequence