Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25540 and BWI76_RS25545 are separated by 110 nucleotides BWI76_RS25545 and BWI76_RS25550 overlap by 11 nucleotides BWI76_RS25550 and BWI76_RS25555 are separated by 11 nucleotides
BWI76_RS25540: BWI76_RS25540 - acetyl-CoA carboxylase biotin carboxylase subunit, at 5,307,543 to 5,308,892
_RS25540
BWI76_RS25545: BWI76_RS25545 - hypothetical protein, at 5,309,003 to 5,309,245
_RS25545
BWI76_RS25550: BWI76_RS25550 - sodium:pantothenate symporter, at 5,309,235 to 5,310,686
_RS25550
BWI76_RS25555: BWI76_RS25555 - 50S ribosomal protein L11 methyltransferase, at 5,310,698 to 5,311,579
_RS25555
Position (kb) 5309
5310
5311 Strain fitness (log2 ratio) -1
0
1
2
3 at 5308.950 kb on + strand at 5309.026 kb on - strand at 5309.031 kb on - strand, within BWI76_RS25545 at 5309.136 kb on + strand, within BWI76_RS25545 at 5309.182 kb on + strand, within BWI76_RS25545 at 5309.192 kb on + strand, within BWI76_RS25545 at 5309.235 kb on - strand at 5309.294 kb on - strand at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.744 kb on + strand, within BWI76_RS25550 at 5309.745 kb on + strand, within BWI76_RS25550 at 5309.747 kb on + strand, within BWI76_RS25550 at 5309.799 kb on + strand, within BWI76_RS25550 at 5309.807 kb on - strand, within BWI76_RS25550 at 5309.974 kb on + strand, within BWI76_RS25550 at 5309.982 kb on - strand, within BWI76_RS25550 at 5309.982 kb on - strand, within BWI76_RS25550 at 5310.121 kb on - strand, within BWI76_RS25550 at 5310.213 kb on + strand, within BWI76_RS25550 at 5310.303 kb on - strand, within BWI76_RS25550 at 5310.447 kb on - strand, within BWI76_RS25550 at 5310.482 kb on - strand, within BWI76_RS25550 at 5310.537 kb on + strand, within BWI76_RS25550 at 5311.003 kb on + strand, within BWI76_RS25555 at 5311.285 kb on - strand, within BWI76_RS25555 at 5311.339 kb on + strand, within BWI76_RS25555 at 5311.469 kb on + strand, within BWI76_RS25555 at 5311.541 kb on + strand at 5311.669 kb on + strand at 5311.669 kb on + strand at 5311.670 kb on - strand at 5311.677 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,308,950 + -0.4 5,309,026 - +3.2 5,309,031 - BWI76_RS25545 0.12 +1.2 5,309,136 + BWI76_RS25545 0.55 +1.0 5,309,182 + BWI76_RS25545 0.74 +0.2 5,309,192 + BWI76_RS25545 0.78 +0.9 5,309,235 - +1.4 5,309,294 - +0.3 5,309,405 - BWI76_RS25550 0.12 -0.0 5,309,405 - BWI76_RS25550 0.12 +0.9 5,309,405 - BWI76_RS25550 0.12 +0.3 5,309,744 + BWI76_RS25550 0.35 +0.2 5,309,745 + BWI76_RS25550 0.35 +0.9 5,309,747 + BWI76_RS25550 0.35 -0.2 5,309,799 + BWI76_RS25550 0.39 +0.3 5,309,807 - BWI76_RS25550 0.39 +0.1 5,309,974 + BWI76_RS25550 0.51 -0.6 5,309,982 - BWI76_RS25550 0.51 +1.7 5,309,982 - BWI76_RS25550 0.51 -0.0 5,310,121 - BWI76_RS25550 0.61 +0.7 5,310,213 + BWI76_RS25550 0.67 +0.1 5,310,303 - BWI76_RS25550 0.74 -0.5 5,310,447 - BWI76_RS25550 0.83 +1.0 5,310,482 - BWI76_RS25550 0.86 +0.4 5,310,537 + BWI76_RS25550 0.90 -1.1 5,311,003 + BWI76_RS25555 0.35 -0.1 5,311,285 - BWI76_RS25555 0.67 -0.3 5,311,339 + BWI76_RS25555 0.73 +0.3 5,311,469 + BWI76_RS25555 0.87 +0.2 5,311,541 + -0.1 5,311,669 + +0.4 5,311,669 + -0.1 5,311,670 - -0.6 5,311,677 - +0.1
Or see this region's nucleotide sequence