Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25515 and BWI76_RS25520 overlap by 1 nucleotides BWI76_RS25520 and BWI76_RS25525 are separated by 121 nucleotides BWI76_RS25525 and BWI76_RS25530 are separated by 9 nucleotides BWI76_RS25530 and BWI76_RS25535 overlap by 28 nucleotides BWI76_RS25535 and BWI76_RS25540 are separated by 10 nucleotides
BWI76_RS25515: BWI76_RS25515 - sulfoxide reductase, at 5,305,065 to 5,306,066
_RS25515
BWI76_RS25520: BWI76_RS25520 - sulfoxide reductase heme-binding subunit YedZ, at 5,306,066 to 5,306,665
_RS25520
BWI76_RS25525: BWI76_RS25525 - hypothetical protein, at 5,306,787 to 5,306,987
_RS25525
BWI76_RS25530: BWI76_RS25530 - acetyl-CoA carboxylase, at 5,306,997 to 5,307,086
_RS25530
BWI76_RS25535: BWI76_RS25535 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 5,307,059 to 5,307,532
_RS25535
BWI76_RS25540: BWI76_RS25540 - acetyl-CoA carboxylase biotin carboxylase subunit, at 5,307,543 to 5,308,892
_RS25540
Position (kb) 5306
5307
5308 Strain fitness (log2 ratio) -1
0
1 at 5306.162 kb on - strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.346 kb on + strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.354 kb on - strand, within BWI76_RS25520 at 5306.386 kb on + strand, within BWI76_RS25520 at 5306.503 kb on + strand, within BWI76_RS25520 at 5306.575 kb on + strand, within BWI76_RS25520
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 5,306,162 - BWI76_RS25520 0.16 +0.8 5,306,346 + BWI76_RS25520 0.47 +0.2 5,306,346 + BWI76_RS25520 0.47 +0.6 5,306,346 + BWI76_RS25520 0.47 +0.2 5,306,346 + BWI76_RS25520 0.47 -0.5 5,306,346 + BWI76_RS25520 0.47 +1.0 5,306,354 - BWI76_RS25520 0.48 -0.1 5,306,354 - BWI76_RS25520 0.48 -0.1 5,306,354 - BWI76_RS25520 0.48 +1.6 5,306,354 - BWI76_RS25520 0.48 +0.2 5,306,354 - BWI76_RS25520 0.48 +1.3 5,306,386 + BWI76_RS25520 0.53 -0.2 5,306,503 + BWI76_RS25520 0.73 -0.6 5,306,575 + BWI76_RS25520 0.85 +0.6
Or see this region's nucleotide sequence