Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22105 and BWI76_RS22110 overlap by 22 nucleotides BWI76_RS22110 and BWI76_RS22115 are separated by 57 nucleotides BWI76_RS22115 and BWI76_RS22120 are separated by 165 nucleotides
BWI76_RS22105: BWI76_RS22105 - 2-isopropylmalate synthase, at 4,614,684 to 4,616,360
_RS22105
BWI76_RS22110: BWI76_RS22110 - putative helix-turn-helix AraC-type transcriptional regulator, at 4,616,339 to 4,617,106
_RS22110
BWI76_RS22115: BWI76_RS22115 - S-ribosylhomocysteine lyase, at 4,617,164 to 4,617,679
_RS22115
BWI76_RS22120: BWI76_RS22120 - glutamate--cysteine ligase, at 4,617,845 to 4,619,401
_RS22120
Position (kb) 4616
4617
4618 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4615.343 kb on + strand, within BWI76_RS22105 at 4615.344 kb on - strand, within BWI76_RS22105 at 4615.344 kb on - strand, within BWI76_RS22105 at 4615.351 kb on - strand, within BWI76_RS22105 at 4615.351 kb on - strand, within BWI76_RS22105 at 4615.753 kb on + strand, within BWI76_RS22105 at 4615.800 kb on + strand, within BWI76_RS22105 at 4616.134 kb on + strand, within BWI76_RS22105 at 4616.446 kb on + strand, within BWI76_RS22110 at 4616.842 kb on + strand, within BWI76_RS22110 at 4616.860 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.887 kb on + strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.895 kb on - strand, within BWI76_RS22110 at 4616.969 kb on - strand, within BWI76_RS22110 at 4617.301 kb on + strand, within BWI76_RS22115 at 4617.473 kb on + strand, within BWI76_RS22115 at 4617.598 kb on - strand, within BWI76_RS22115 at 4617.625 kb on + strand, within BWI76_RS22115 at 4617.671 kb on - strand at 4617.737 kb on + strand at 4617.755 kb on - strand at 4617.987 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 4,615,343 + BWI76_RS22105 0.39 -0.3 4,615,344 - BWI76_RS22105 0.39 +0.1 4,615,344 - BWI76_RS22105 0.39 +0.1 4,615,351 - BWI76_RS22105 0.40 +0.2 4,615,351 - BWI76_RS22105 0.40 -0.2 4,615,753 + BWI76_RS22105 0.64 -0.6 4,615,800 + BWI76_RS22105 0.67 -2.8 4,616,134 + BWI76_RS22105 0.86 -0.2 4,616,446 + BWI76_RS22110 0.14 +0.9 4,616,842 + BWI76_RS22110 0.65 +1.6 4,616,860 + BWI76_RS22110 0.68 +0.8 4,616,887 + BWI76_RS22110 0.71 +1.1 4,616,887 + BWI76_RS22110 0.71 +0.7 4,616,887 + BWI76_RS22110 0.71 +0.4 4,616,895 - BWI76_RS22110 0.72 -1.2 4,616,895 - BWI76_RS22110 0.72 -0.8 4,616,969 - BWI76_RS22110 0.82 +0.4 4,617,301 + BWI76_RS22115 0.27 -1.1 4,617,473 + BWI76_RS22115 0.60 +0.0 4,617,598 - BWI76_RS22115 0.84 -0.6 4,617,625 + BWI76_RS22115 0.89 +1.0 4,617,671 - +0.6 4,617,737 + -0.2 4,617,755 - +0.1 4,617,987 - -1.8
Or see this region's nucleotide sequence