Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS18595 and BWI76_RS18600 are separated by 14 nucleotides BWI76_RS18600 and BWI76_RS18605 overlap by 11 nucleotides BWI76_RS18605 and BWI76_RS18610 are separated by 178 nucleotides
BWI76_RS18595: BWI76_RS18595 - hypothetical protein, at 3,856,711 to 3,857,337
_RS18595
BWI76_RS18600: BWI76_RS18600 - HupC/HyaC/HydC family protein, at 3,857,352 to 3,857,960
_RS18600
BWI76_RS18605: BWI76_RS18605 - oxidoreductase molybdopterin-binding protein, at 3,857,950 to 3,858,717
_RS18605
BWI76_RS18610: BWI76_RS18610 - transcriptional regulator LysR, at 3,858,896 to 3,859,882
_RS18610
Position (kb) 3857
3858
3859 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 3857.059 kb on + strand, within BWI76_RS18595 at 3857.237 kb on - strand, within BWI76_RS18595 at 3857.384 kb on - strand at 3857.398 kb on - strand at 3857.416 kb on - strand, within BWI76_RS18600 at 3857.540 kb on + strand, within BWI76_RS18600 at 3857.572 kb on - strand, within BWI76_RS18600 at 3857.585 kb on + strand, within BWI76_RS18600 at 3857.676 kb on - strand, within BWI76_RS18600 at 3857.737 kb on - strand, within BWI76_RS18600 at 3857.795 kb on + strand, within BWI76_RS18600 at 3858.065 kb on + strand, within BWI76_RS18605 at 3858.180 kb on + strand, within BWI76_RS18605 at 3858.189 kb on - strand, within BWI76_RS18605 at 3858.685 kb on + strand at 3859.038 kb on + strand, within BWI76_RS18610 at 3859.334 kb on + strand, within BWI76_RS18610 at 3859.580 kb on - strand, within BWI76_RS18610 at 3859.677 kb on - strand, within BWI76_RS18610
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,857,059 + BWI76_RS18595 0.56 -0.3 3,857,237 - BWI76_RS18595 0.84 -0.3 3,857,384 - -1.2 3,857,398 - +0.9 3,857,416 - BWI76_RS18600 0.11 +0.7 3,857,540 + BWI76_RS18600 0.31 +1.3 3,857,572 - BWI76_RS18600 0.36 +0.2 3,857,585 + BWI76_RS18600 0.38 +0.7 3,857,676 - BWI76_RS18600 0.53 -1.8 3,857,737 - BWI76_RS18600 0.63 -0.1 3,857,795 + BWI76_RS18600 0.73 -0.9 3,858,065 + BWI76_RS18605 0.15 +3.0 3,858,180 + BWI76_RS18605 0.30 -2.3 3,858,189 - BWI76_RS18605 0.31 -3.2 3,858,685 + -0.9 3,859,038 + BWI76_RS18610 0.14 -0.7 3,859,334 + BWI76_RS18610 0.44 +0.3 3,859,580 - BWI76_RS18610 0.69 -0.5 3,859,677 - BWI76_RS18610 0.79 +0.4
Or see this region's nucleotide sequence