Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17290 and BWI76_RS17295 are separated by 159 nucleotides BWI76_RS17295 and BWI76_RS17300 are separated by 35 nucleotides BWI76_RS17300 and BWI76_RS17310 are separated by 282 nucleotides BWI76_RS17310 and BWI76_RS17315 are separated by 107 nucleotides
BWI76_RS17290: BWI76_RS17290 - formyltetrahydrofolate deformylase, at 3,582,460 to 3,583,302
_RS17290
BWI76_RS17295: BWI76_RS17295 - tRNA-Tyr, at 3,583,462 to 3,583,546
_RS17295
BWI76_RS17300: BWI76_RS17300 - tRNA-Tyr, at 3,583,582 to 3,583,666
_RS17300
BWI76_RS17310: BWI76_RS17310 - AsnC family transcriptional regulator, at 3,583,949 to 3,584,392
_RS17310
BWI76_RS17315: BWI76_RS17315 - EamA family transporter, at 3,584,500 to 3,585,395
_RS17315
Position (kb) 3583
3584 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 3582.663 kb on - strand, within BWI76_RS17290 at 3582.663 kb on - strand at 3582.706 kb on - strand, within BWI76_RS17290 at 3582.763 kb on - strand, within BWI76_RS17290 at 3583.036 kb on - strand, within BWI76_RS17290 at 3583.176 kb on + strand, within BWI76_RS17290 at 3583.443 kb on + strand at 3583.607 kb on - strand, within BWI76_RS17300 at 3583.622 kb on - strand, within BWI76_RS17300 at 3583.819 kb on + strand at 3583.905 kb on + strand at 3583.967 kb on + strand at 3584.037 kb on + strand, within BWI76_RS17310 at 3584.196 kb on + strand, within BWI76_RS17310 at 3584.209 kb on - strand, within BWI76_RS17310 at 3584.262 kb on - strand, within BWI76_RS17310 at 3584.262 kb on - strand, within BWI76_RS17310 at 3584.479 kb on - strand at 3584.626 kb on - strand, within BWI76_RS17315
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,582,663 - BWI76_RS17290 0.24 -1.7 3,582,663 - -0.1 3,582,706 - BWI76_RS17290 0.29 +0.3 3,582,763 - BWI76_RS17290 0.36 +0.7 3,583,036 - BWI76_RS17290 0.68 +0.7 3,583,176 + BWI76_RS17290 0.85 +0.6 3,583,443 + +0.4 3,583,607 - BWI76_RS17300 0.29 +0.9 3,583,622 - BWI76_RS17300 0.47 -3.0 3,583,819 + +0.3 3,583,905 + +0.3 3,583,967 + +0.0 3,584,037 + BWI76_RS17310 0.20 -0.6 3,584,196 + BWI76_RS17310 0.56 -1.8 3,584,209 - BWI76_RS17310 0.59 -0.7 3,584,262 - BWI76_RS17310 0.70 -0.7 3,584,262 - BWI76_RS17310 0.70 +0.9 3,584,479 - +1.2 3,584,626 - BWI76_RS17315 0.14 +0.3
Or see this region's nucleotide sequence