Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17185 and BWI76_RS17190 are separated by 19 nucleotides BWI76_RS17190 and BWI76_RS17195 are separated by 211 nucleotides BWI76_RS17195 and BWI76_RS17200 are separated by 58 nucleotides BWI76_RS17200 and BWI76_RS17205 are separated by 32 nucleotides
BWI76_RS17185: BWI76_RS17185 - fructosamine kinase family protein, at 3,561,425 to 3,562,297
_RS17185
BWI76_RS17190: BWI76_RS17190 - TonB system transport protein TonB, at 3,562,317 to 3,563,054
_RS17190
BWI76_RS17195: BWI76_RS17195 - hypothetical protein, at 3,563,266 to 3,563,562
_RS17195
BWI76_RS17200: BWI76_RS17200 - hypothetical protein, at 3,563,621 to 3,563,905
_RS17200
BWI76_RS17205: BWI76_RS17205 - cardiolipin synthase A, at 3,563,938 to 3,565,398
_RS17205
Position (kb) 3563
3564 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3562.548 kb on + strand, within BWI76_RS17190 at 3562.960 kb on - strand, within BWI76_RS17190 at 3562.965 kb on - strand, within BWI76_RS17190 at 3563.466 kb on + strand, within BWI76_RS17195 at 3563.639 kb on + strand at 3563.776 kb on - strand, within BWI76_RS17200 at 3563.964 kb on - strand at 3563.964 kb on - strand at 3563.978 kb on - strand at 3564.296 kb on + strand, within BWI76_RS17205 at 3564.342 kb on - strand, within BWI76_RS17205 at 3564.389 kb on - strand, within BWI76_RS17205
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,562,548 + BWI76_RS17190 0.31 +0.9 3,562,960 - BWI76_RS17190 0.87 -1.9 3,562,965 - BWI76_RS17190 0.88 -0.2 3,563,466 + BWI76_RS17195 0.67 -1.1 3,563,639 + +2.5 3,563,776 - BWI76_RS17200 0.54 +0.4 3,563,964 - +0.3 3,563,964 - +0.5 3,563,978 - -1.4 3,564,296 + BWI76_RS17205 0.25 -0.6 3,564,342 - BWI76_RS17205 0.28 -1.4 3,564,389 - BWI76_RS17205 0.31 +0.3
Or see this region's nucleotide sequence