Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16055

Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16045 and BWI76_RS16050 are separated by 2 nucleotidesBWI76_RS16050 and BWI76_RS16055 overlap by 4 nucleotidesBWI76_RS16055 and BWI76_RS16060 overlap by 11 nucleotides BWI76_RS16045: BWI76_RS16045 - transporter, at 3,326,687 to 3,327,646 _RS16045 BWI76_RS16050: BWI76_RS16050 - malonate decarboxylase holo-ACP synthase, at 3,327,649 to 3,328,269 _RS16050 BWI76_RS16055: BWI76_RS16055 - malonate decarboxylase subunit epsilon, at 3,328,266 to 3,329,168 _RS16055 BWI76_RS16060: BWI76_RS16060 - LysR family transcriptional regulator, at 3,329,158 to 3,330,084 _RS16060 Position (kb) 3328 3329 3330Strain fitness (log2 ratio) -2 -1 0 1 2at 3327.330 kb on + strand, within BWI76_RS16045at 3327.471 kb on + strand, within BWI76_RS16045at 3327.735 kb on + strand, within BWI76_RS16050at 3327.812 kb on - strand, within BWI76_RS16050at 3327.883 kb on - strand, within BWI76_RS16050at 3328.433 kb on - strand, within BWI76_RS16055at 3328.607 kb on + strand, within BWI76_RS16055at 3328.703 kb on + strand, within BWI76_RS16055at 3328.905 kb on + strand, within BWI76_RS16055at 3328.913 kb on - strand, within BWI76_RS16055at 3328.942 kb on - strand, within BWI76_RS16055at 3329.424 kb on + strand, within BWI76_RS16060at 3329.432 kb on - strand, within BWI76_RS16060at 3329.522 kb on + strand, within BWI76_RS16060at 3329.564 kb on + strand, within BWI76_RS16060at 3329.756 kb on - strand, within BWI76_RS16060at 3329.948 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
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3,327,330 + BWI76_RS16045 0.67 -1.9
3,327,471 + BWI76_RS16045 0.82 +1.1
3,327,735 + BWI76_RS16050 0.14 +0.3
3,327,812 - BWI76_RS16050 0.26 -0.5
3,327,883 - BWI76_RS16050 0.38 +0.7
3,328,433 - BWI76_RS16055 0.18 +1.3
3,328,607 + BWI76_RS16055 0.38 +0.7
3,328,703 + BWI76_RS16055 0.48 -0.4
3,328,905 + BWI76_RS16055 0.71 +0.6
3,328,913 - BWI76_RS16055 0.72 +1.5
3,328,942 - BWI76_RS16055 0.75 +0.3
3,329,424 + BWI76_RS16060 0.29 +0.2
3,329,432 - BWI76_RS16060 0.30 -1.9
3,329,522 + BWI76_RS16060 0.39 -0.1
3,329,564 + BWI76_RS16060 0.44 -1.6
3,329,756 - BWI76_RS16060 0.65 +0.2
3,329,948 - +2.1

Or see this region's nucleotide sequence