Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15820 and BWI76_RS15825 are separated by 427 nucleotides BWI76_RS15825 and BWI76_RS15830 are separated by 88 nucleotides BWI76_RS15830 and BWI76_RS15835 are separated by 7 nucleotides BWI76_RS15835 and BWI76_RS15840 are separated by 151 nucleotides
BWI76_RS15820: BWI76_RS15820 - hypothetical protein, at 3,289,748 to 3,289,951
_RS15820
BWI76_RS15825: BWI76_RS15825 - hypothetical protein, at 3,290,379 to 3,290,573
_RS15825
BWI76_RS15830: BWI76_RS15830 - hypothetical protein, at 3,290,662 to 3,291,666
_RS15830
BWI76_RS15835: BWI76_RS15835 - hypothetical protein, at 3,291,674 to 3,291,871
_RS15835
BWI76_RS15840: BWI76_RS15840 - prophage protein, at 3,292,023 to 3,292,805
_RS15840
Position (kb) 3290
3291
3292 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 3289.664 kb on + strand at 3289.672 kb on - strand at 3289.753 kb on - strand at 3290.312 kb on + strand at 3290.455 kb on + strand, within BWI76_RS15825 at 3290.553 kb on + strand, within BWI76_RS15825 at 3290.576 kb on + strand at 3290.743 kb on + strand at 3290.827 kb on + strand, within BWI76_RS15830 at 3290.862 kb on - strand, within BWI76_RS15830 at 3290.869 kb on + strand, within BWI76_RS15830 at 3290.869 kb on + strand, within BWI76_RS15830 at 3290.877 kb on - strand, within BWI76_RS15830 at 3290.889 kb on + strand, within BWI76_RS15830 at 3291.006 kb on + strand, within BWI76_RS15830 at 3291.068 kb on - strand, within BWI76_RS15830 at 3291.241 kb on + strand, within BWI76_RS15830 at 3291.274 kb on - strand, within BWI76_RS15830 at 3291.377 kb on - strand, within BWI76_RS15830 at 3291.400 kb on + strand, within BWI76_RS15830 at 3291.535 kb on - strand, within BWI76_RS15830 at 3291.559 kb on - strand, within BWI76_RS15830 at 3291.559 kb on - strand, within BWI76_RS15830 at 3291.589 kb on - strand at 3291.708 kb on - strand, within BWI76_RS15835 at 3291.716 kb on - strand, within BWI76_RS15835 at 3291.801 kb on + strand, within BWI76_RS15835 at 3291.830 kb on + strand, within BWI76_RS15835 at 3292.119 kb on + strand, within BWI76_RS15840 at 3292.119 kb on + strand, within BWI76_RS15840 at 3292.126 kb on - strand, within BWI76_RS15840 at 3292.127 kb on - strand, within BWI76_RS15840 at 3292.295 kb on + strand, within BWI76_RS15840 at 3292.349 kb on + strand, within BWI76_RS15840 at 3292.349 kb on - strand, within BWI76_RS15840 at 3292.520 kb on + strand, within BWI76_RS15840 at 3292.520 kb on + strand, within BWI76_RS15840 at 3292.520 kb on + strand, within BWI76_RS15840 at 3292.528 kb on - strand, within BWI76_RS15840 at 3292.528 kb on - strand, within BWI76_RS15840
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,289,664 + -1.5 3,289,672 - -0.3 3,289,753 - -1.1 3,290,312 + -2.0 3,290,455 + BWI76_RS15825 0.39 -0.5 3,290,553 + BWI76_RS15825 0.89 -3.4 3,290,576 + -3.7 3,290,743 + -6.1 3,290,827 + BWI76_RS15830 0.16 -5.7 3,290,862 - BWI76_RS15830 0.20 -1.3 3,290,869 + BWI76_RS15830 0.21 -4.4 3,290,869 + BWI76_RS15830 0.21 -2.4 3,290,877 - BWI76_RS15830 0.21 -2.6 3,290,889 + BWI76_RS15830 0.23 -3.9 3,291,006 + BWI76_RS15830 0.34 -1.7 3,291,068 - BWI76_RS15830 0.40 +0.2 3,291,241 + BWI76_RS15830 0.58 -4.0 3,291,274 - BWI76_RS15830 0.61 -0.4 3,291,377 - BWI76_RS15830 0.71 -0.5 3,291,400 + BWI76_RS15830 0.73 +1.1 3,291,535 - BWI76_RS15830 0.87 -1.5 3,291,559 - BWI76_RS15830 0.89 -0.6 3,291,559 - BWI76_RS15830 0.89 +0.0 3,291,589 - -1.5 3,291,708 - BWI76_RS15835 0.17 -0.4 3,291,716 - BWI76_RS15835 0.21 +2.1 3,291,801 + BWI76_RS15835 0.64 +1.1 3,291,830 + BWI76_RS15835 0.79 -0.9 3,292,119 + BWI76_RS15840 0.12 -2.7 3,292,119 + BWI76_RS15840 0.12 -4.8 3,292,126 - BWI76_RS15840 0.13 -0.6 3,292,127 - BWI76_RS15840 0.13 -0.3 3,292,295 + BWI76_RS15840 0.35 -3.3 3,292,349 + BWI76_RS15840 0.42 -0.7 3,292,349 - BWI76_RS15840 0.42 -0.8 3,292,520 + BWI76_RS15840 0.63 +0.1 3,292,520 + BWI76_RS15840 0.63 -3.0 3,292,520 + BWI76_RS15840 0.63 +1.1 3,292,528 - BWI76_RS15840 0.64 -0.5 3,292,528 - BWI76_RS15840 0.64 -2.2
Or see this region's nucleotide sequence