Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15710 and BWI76_RS15715 are separated by 133 nucleotides BWI76_RS15715 and BWI76_RS15720 overlap by 8 nucleotides BWI76_RS15720 and BWI76_RS15725 overlap by 1 nucleotides BWI76_RS15725 and BWI76_RS15730 are separated by 260 nucleotides
BWI76_RS15710: BWI76_RS15710 - phage antitermination protein Q, at 3,277,897 to 3,278,586
_RS15710
BWI76_RS15715: BWI76_RS15715 - putative ninG protein, at 3,278,720 to 3,279,358
_RS15715
BWI76_RS15720: BWI76_RS15720 - prophage protein NinE, at 3,279,351 to 3,279,521
_RS15720
BWI76_RS15725: BWI76_RS15725 - hypothetical protein, at 3,279,521 to 3,279,976
_RS15725
BWI76_RS15730: BWI76_RS15730 - hypothetical protein, at 3,280,237 to 3,280,467
_RS15730
Position (kb) 3278
3279
3280 Strain fitness (log2 ratio) -1
0
1 at 3277.778 kb on + strand at 3278.372 kb on + strand, within BWI76_RS15710 at 3278.474 kb on - strand, within BWI76_RS15710 at 3278.608 kb on - strand at 3278.656 kb on - strand at 3278.863 kb on - strand, within BWI76_RS15715 at 3279.374 kb on - strand, within BWI76_RS15720 at 3279.448 kb on - strand, within BWI76_RS15720 at 3279.550 kb on - strand at 3279.838 kb on + strand, within BWI76_RS15725 at 3279.846 kb on - strand, within BWI76_RS15725 at 3279.864 kb on + strand, within BWI76_RS15725 at 3279.949 kb on + strand at 3280.350 kb on - strand, within BWI76_RS15730
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 3,277,778 + -1.6 3,278,372 + BWI76_RS15710 0.69 -1.7 3,278,474 - BWI76_RS15710 0.84 +0.4 3,278,608 - -1.3 3,278,656 - -0.9 3,278,863 - BWI76_RS15715 0.22 -1.1 3,279,374 - BWI76_RS15720 0.13 -0.9 3,279,448 - BWI76_RS15720 0.57 -0.6 3,279,550 - -0.5 3,279,838 + BWI76_RS15725 0.70 -0.4 3,279,846 - BWI76_RS15725 0.71 +0.2 3,279,864 + BWI76_RS15725 0.75 -0.1 3,279,949 + -0.7 3,280,350 - BWI76_RS15730 0.49 -0.7
Or see this region's nucleotide sequence