Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13665 and BWI76_RS13670 are separated by 97 nucleotides BWI76_RS13670 and BWI76_RS13675 are separated by 189 nucleotides BWI76_RS13675 and BWI76_RS13680 are separated by 119 nucleotides
BWI76_RS13665: BWI76_RS13665 - nitrate reductase subunit alpha, at 2,848,370 to 2,852,110
_RS13665
BWI76_RS13670: BWI76_RS13670 - NarK family nitrate/nitrite MFS transporter, at 2,852,208 to 2,853,596
_RS13670
BWI76_RS13675: BWI76_RS13675 - TetR family transcriptional regulator, at 2,853,786 to 2,854,361
_RS13675
BWI76_RS13680: BWI76_RS13680 - methyl viologen resistance protein SmvA, at 2,854,481 to 2,855,971
_RS13680
Position (kb)
2852
2853
2854 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2851.241 kb on - strand, within BWI76_RS13665 at 2851.581 kb on + strand, within BWI76_RS13665 at 2851.730 kb on + strand, within BWI76_RS13665 at 2851.812 kb on - strand at 2851.866 kb on - strand at 2852.101 kb on - strand at 2852.266 kb on + strand at 2852.580 kb on - strand, within BWI76_RS13670 at 2852.709 kb on - strand, within BWI76_RS13670 at 2852.887 kb on - strand, within BWI76_RS13670 at 2852.909 kb on + strand, within BWI76_RS13670 at 2853.073 kb on - strand, within BWI76_RS13670 at 2853.099 kb on + strand, within BWI76_RS13670 at 2853.099 kb on + strand, within BWI76_RS13670 at 2853.099 kb on + strand, within BWI76_RS13670 at 2853.104 kb on + strand, within BWI76_RS13670 at 2853.106 kb on + strand, within BWI76_RS13670 at 2853.107 kb on - strand, within BWI76_RS13670 at 2853.194 kb on - strand, within BWI76_RS13670 at 2853.194 kb on - strand, within BWI76_RS13670 at 2853.228 kb on + strand, within BWI76_RS13670 at 2853.231 kb on + strand, within BWI76_RS13670 at 2853.231 kb on + strand, within BWI76_RS13670 at 2853.231 kb on + strand, within BWI76_RS13670 at 2853.233 kb on + strand, within BWI76_RS13670 at 2853.233 kb on + strand, within BWI76_RS13670 at 2853.233 kb on + strand, within BWI76_RS13670 at 2853.239 kb on - strand, within BWI76_RS13670 at 2853.239 kb on - strand, within BWI76_RS13670 at 2853.239 kb on - strand, within BWI76_RS13670 at 2853.239 kb on - strand, within BWI76_RS13670 at 2853.239 kb on - strand, within BWI76_RS13670 at 2853.239 kb on - strand, within BWI76_RS13670 at 2853.241 kb on + strand, within BWI76_RS13670 at 2853.241 kb on - strand, within BWI76_RS13670 at 2853.241 kb on - strand, within BWI76_RS13670 at 2853.241 kb on - strand, within BWI76_RS13670 at 2853.241 kb on - strand, within BWI76_RS13670 at 2853.246 kb on - strand, within BWI76_RS13670 at 2853.252 kb on + strand, within BWI76_RS13670 at 2853.255 kb on + strand, within BWI76_RS13670 at 2853.256 kb on - strand, within BWI76_RS13670 at 2853.260 kb on - strand, within BWI76_RS13670 at 2853.261 kb on - strand, within BWI76_RS13670 at 2853.289 kb on + strand, within BWI76_RS13670 at 2853.292 kb on - strand, within BWI76_RS13670 at 2853.317 kb on + strand, within BWI76_RS13670 at 2853.332 kb on - strand, within BWI76_RS13670 at 2853.344 kb on - strand, within BWI76_RS13670 at 2853.441 kb on - strand, within BWI76_RS13670 at 2853.601 kb on - strand at 2853.623 kb on - strand at 2853.689 kb on - strand at 2853.693 kb on + strand at 2853.701 kb on - strand at 2853.774 kb on + strand at 2853.781 kb on - strand at 2853.782 kb on - strand at 2853.782 kb on - strand at 2853.880 kb on + strand, within BWI76_RS13675 at 2853.888 kb on - strand, within BWI76_RS13675 at 2853.888 kb on - strand, within BWI76_RS13675 at 2853.931 kb on + strand, within BWI76_RS13675 at 2853.939 kb on - strand, within BWI76_RS13675 at 2853.955 kb on - strand, within BWI76_RS13675 at 2853.988 kb on - strand, within BWI76_RS13675 at 2853.996 kb on - strand, within BWI76_RS13675 at 2854.015 kb on + strand, within BWI76_RS13675 at 2854.015 kb on + strand, within BWI76_RS13675 at 2854.015 kb on + strand, within BWI76_RS13675 at 2854.023 kb on - strand, within BWI76_RS13675 at 2854.023 kb on - strand, within BWI76_RS13675 at 2854.023 kb on - strand, within BWI76_RS13675 at 2854.193 kb on - strand, within BWI76_RS13675 at 2854.219 kb on + strand, within BWI76_RS13675 at 2854.221 kb on - strand, within BWI76_RS13675 at 2854.483 kb on - strand at 2854.580 kb on - strand at 2854.586 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 2,851,241 - BWI76_RS13665 0.77 -0.2 2,851,581 + BWI76_RS13665 0.86 -0.0 2,851,730 + BWI76_RS13665 0.90 -0.1 2,851,812 - +0.4 2,851,866 - +0.3 2,852,101 - -0.0 2,852,266 + +0.8 2,852,580 - BWI76_RS13670 0.27 -1.8 2,852,709 - BWI76_RS13670 0.36 -2.7 2,852,887 - BWI76_RS13670 0.49 -0.0 2,852,909 + BWI76_RS13670 0.50 -0.6 2,853,073 - BWI76_RS13670 0.62 +3.3 2,853,099 + BWI76_RS13670 0.64 -0.5 2,853,099 + BWI76_RS13670 0.64 -0.6 2,853,099 + BWI76_RS13670 0.64 -0.0 2,853,104 + BWI76_RS13670 0.65 -2.2 2,853,106 + BWI76_RS13670 0.65 -1.1 2,853,107 - BWI76_RS13670 0.65 -1.6 2,853,194 - BWI76_RS13670 0.71 -2.8 2,853,194 - BWI76_RS13670 0.71 -2.8 2,853,228 + BWI76_RS13670 0.73 -1.1 2,853,231 + BWI76_RS13670 0.74 -0.2 2,853,231 + BWI76_RS13670 0.74 +0.2 2,853,231 + BWI76_RS13670 0.74 +0.5 2,853,233 + BWI76_RS13670 0.74 -2.0 2,853,233 + BWI76_RS13670 0.74 +1.0 2,853,233 + BWI76_RS13670 0.74 +0.2 2,853,239 - BWI76_RS13670 0.74 +1.0 2,853,239 - BWI76_RS13670 0.74 +0.6 2,853,239 - BWI76_RS13670 0.74 -1.3 2,853,239 - BWI76_RS13670 0.74 -1.9 2,853,239 - BWI76_RS13670 0.74 -2.3 2,853,239 - BWI76_RS13670 0.74 -0.6 2,853,241 + BWI76_RS13670 0.74 +1.6 2,853,241 - BWI76_RS13670 0.74 -0.0 2,853,241 - BWI76_RS13670 0.74 +0.3 2,853,241 - BWI76_RS13670 0.74 -1.6 2,853,241 - BWI76_RS13670 0.74 -0.1 2,853,246 - BWI76_RS13670 0.75 -0.9 2,853,252 + BWI76_RS13670 0.75 -1.4 2,853,255 + BWI76_RS13670 0.75 -0.4 2,853,256 - BWI76_RS13670 0.75 -0.0 2,853,260 - BWI76_RS13670 0.76 -0.0 2,853,261 - BWI76_RS13670 0.76 -1.5 2,853,289 + BWI76_RS13670 0.78 -0.4 2,853,292 - BWI76_RS13670 0.78 -0.0 2,853,317 + BWI76_RS13670 0.80 +0.3 2,853,332 - BWI76_RS13670 0.81 -1.6 2,853,344 - BWI76_RS13670 0.82 +0.2 2,853,441 - BWI76_RS13670 0.89 -0.8 2,853,601 - +0.3 2,853,623 - +0.2 2,853,689 - -0.6 2,853,693 + -0.0 2,853,701 - -0.3 2,853,774 + -1.1 2,853,781 - +0.8 2,853,782 - +0.3 2,853,782 - -1.2 2,853,880 + BWI76_RS13675 0.16 -0.0 2,853,888 - BWI76_RS13675 0.18 +1.9 2,853,888 - BWI76_RS13675 0.18 +0.4 2,853,931 + BWI76_RS13675 0.25 +0.2 2,853,939 - BWI76_RS13675 0.27 -0.1 2,853,955 - BWI76_RS13675 0.29 +1.2 2,853,988 - BWI76_RS13675 0.35 -1.4 2,853,996 - BWI76_RS13675 0.36 +0.4 2,854,015 + BWI76_RS13675 0.40 -1.6 2,854,015 + BWI76_RS13675 0.40 +0.9 2,854,015 + BWI76_RS13675 0.40 -0.6 2,854,023 - BWI76_RS13675 0.41 -0.2 2,854,023 - BWI76_RS13675 0.41 +0.3 2,854,023 - BWI76_RS13675 0.41 +1.2 2,854,193 - BWI76_RS13675 0.71 -0.0 2,854,219 + BWI76_RS13675 0.75 -0.2 2,854,221 - BWI76_RS13675 0.76 -1.6 2,854,483 - -0.0 2,854,580 - +0.5 2,854,586 - +1.2
Or see this region's nucleotide sequence