Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS06775 and BWI76_RS06780 are separated by 22 nucleotides BWI76_RS06780 and BWI76_RS06785 are separated by 142 nucleotides BWI76_RS06785 and BWI76_RS06790 are separated by 130 nucleotides
BWI76_RS06775: BWI76_RS06775 - multidrug efflux RND transporter permease subunit, at 1,418,843 to 1,421,989
_RS06775
BWI76_RS06780: BWI76_RS06780 - MexE family multidrug efflux RND transporter periplasmic adaptor subunit, at 1,422,012 to 1,423,205
_RS06780
BWI76_RS06785: BWI76_RS06785 - DNA-binding transcriptional repressor AcrR, at 1,423,348 to 1,423,998
_RS06785
BWI76_RS06790: BWI76_RS06790 - mechanosensitive channel MscK, at 1,424,129 to 1,427,470
_RS06790
Position (kb) 1422
1423
1424 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1421.142 kb on + strand, within BWI76_RS06775 at 1421.212 kb on + strand, within BWI76_RS06775 at 1421.257 kb on + strand, within BWI76_RS06775 at 1421.450 kb on - strand, within BWI76_RS06775 at 1421.719 kb on - strand at 1422.024 kb on - strand at 1422.090 kb on - strand at 1422.300 kb on + strand, within BWI76_RS06780 at 1422.692 kb on - strand, within BWI76_RS06780 at 1422.782 kb on - strand, within BWI76_RS06780 at 1422.802 kb on + strand, within BWI76_RS06780 at 1422.810 kb on - strand, within BWI76_RS06780 at 1422.831 kb on - strand, within BWI76_RS06780 at 1422.985 kb on + strand, within BWI76_RS06780 at 1423.032 kb on + strand, within BWI76_RS06780 at 1423.034 kb on - strand, within BWI76_RS06780 at 1423.140 kb on - strand at 1423.142 kb on + strand at 1423.161 kb on + strand at 1423.430 kb on + strand, within BWI76_RS06785 at 1423.562 kb on - strand, within BWI76_RS06785 at 1423.660 kb on + strand, within BWI76_RS06785 at 1423.786 kb on - strand, within BWI76_RS06785 at 1423.786 kb on - strand, within BWI76_RS06785 at 1423.906 kb on - strand, within BWI76_RS06785 at 1423.906 kb on - strand, within BWI76_RS06785 at 1423.916 kb on - strand, within BWI76_RS06785 at 1423.916 kb on - strand, within BWI76_RS06785 at 1423.976 kb on + strand at 1424.132 kb on - strand at 1424.132 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 1,421,142 + BWI76_RS06775 0.73 -0.4 1,421,212 + BWI76_RS06775 0.75 -0.2 1,421,257 + BWI76_RS06775 0.77 -1.4 1,421,450 - BWI76_RS06775 0.83 -2.2 1,421,719 - -1.0 1,422,024 - -0.2 1,422,090 - +0.3 1,422,300 + BWI76_RS06780 0.24 +0.1 1,422,692 - BWI76_RS06780 0.57 -2.0 1,422,782 - BWI76_RS06780 0.64 +1.6 1,422,802 + BWI76_RS06780 0.66 +0.1 1,422,810 - BWI76_RS06780 0.67 -1.3 1,422,831 - BWI76_RS06780 0.69 -0.6 1,422,985 + BWI76_RS06780 0.81 -0.1 1,423,032 + BWI76_RS06780 0.85 -2.2 1,423,034 - BWI76_RS06780 0.86 -2.4 1,423,140 - +1.2 1,423,142 + -0.1 1,423,161 + -1.3 1,423,430 + BWI76_RS06785 0.13 +0.1 1,423,562 - BWI76_RS06785 0.33 -0.1 1,423,660 + BWI76_RS06785 0.48 +0.1 1,423,786 - BWI76_RS06785 0.67 -1.5 1,423,786 - BWI76_RS06785 0.67 -1.5 1,423,906 - BWI76_RS06785 0.86 +1.1 1,423,906 - BWI76_RS06785 0.86 +0.5 1,423,916 - BWI76_RS06785 0.87 -0.5 1,423,916 - BWI76_RS06785 0.87 +0.3 1,423,976 + -0.4 1,424,132 - -0.6 1,424,132 - -2.8
Or see this region's nucleotide sequence