Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03510 and BWI76_RS03515 overlap by 4 nucleotides BWI76_RS03515 and BWI76_RS03520 are separated by 531 nucleotides
BWI76_RS03510: BWI76_RS03510 - multidrug ABC transporter ATP-binding protein, at 725,456 to 728,185
_RS03510
BWI76_RS03515: BWI76_RS03515 - hypothetical protein, at 728,182 to 729,249
_RS03515
BWI76_RS03520: BWI76_RS03520 - diguanylate cyclase, at 729,781 to 731,178
_RS03520
Position (kb)
728
729
730 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 727.281 kb on + strand, within BWI76_RS03510 at 727.485 kb on - strand, within BWI76_RS03510 at 727.494 kb on - strand, within BWI76_RS03510 at 727.809 kb on - strand, within BWI76_RS03510 at 727.859 kb on + strand, within BWI76_RS03510 at 727.933 kb on - strand at 727.957 kb on + strand at 728.050 kb on + strand at 728.073 kb on + strand at 728.089 kb on + strand at 728.121 kb on - strand at 728.259 kb on + strand at 728.275 kb on + strand at 728.283 kb on - strand at 728.393 kb on + strand, within BWI76_RS03515 at 728.439 kb on - strand, within BWI76_RS03515 at 728.660 kb on + strand, within BWI76_RS03515 at 729.171 kb on + strand at 729.267 kb on - strand at 729.273 kb on + strand at 729.278 kb on + strand at 729.347 kb on - strand at 729.347 kb on - strand at 729.469 kb on + strand at 729.471 kb on + strand at 729.479 kb on - strand at 729.528 kb on + strand at 729.536 kb on - strand at 729.579 kb on + strand at 729.579 kb on + strand at 729.587 kb on + strand at 729.589 kb on + strand at 729.595 kb on - strand at 729.595 kb on - strand at 729.631 kb on - strand at 729.646 kb on - strand at 729.646 kb on - strand at 729.654 kb on - strand at 729.658 kb on + strand at 729.659 kb on + strand at 729.667 kb on - strand at 729.667 kb on - strand at 729.713 kb on + strand at 729.713 kb on + strand at 729.720 kb on - strand at 729.721 kb on - strand at 729.721 kb on - strand at 729.726 kb on + strand at 729.726 kb on + strand at 729.732 kb on - strand at 729.734 kb on + strand at 729.734 kb on - strand at 729.744 kb on - strand at 729.757 kb on + strand at 729.757 kb on + strand at 729.757 kb on + strand at 729.759 kb on + strand at 729.759 kb on + strand at 729.759 kb on + strand at 729.759 kb on + strand at 729.765 kb on - strand at 729.767 kb on - strand at 729.767 kb on - strand at 729.767 kb on - strand at 729.772 kb on - strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.835 kb on + strand at 729.837 kb on + strand at 729.839 kb on + strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.843 kb on - strand at 729.845 kb on + strand at 729.845 kb on + strand at 729.845 kb on + strand at 729.845 kb on + strand at 729.845 kb on + strand at 729.845 kb on + strand at 729.845 kb on - strand at 729.845 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.853 kb on - strand at 729.857 kb on + strand at 729.858 kb on + strand at 729.858 kb on + strand at 729.858 kb on + strand at 729.858 kb on + strand at 729.858 kb on + strand at 729.858 kb on + strand at 729.858 kb on + strand at 729.859 kb on - strand at 729.861 kb on - strand at 729.866 kb on - strand at 729.866 kb on - strand at 729.878 kb on + strand at 729.878 kb on - strand at 729.894 kb on + strand at 729.895 kb on + strand at 729.901 kb on - strand at 729.955 kb on + strand, within BWI76_RS03520 at 729.973 kb on + strand, within BWI76_RS03520 at 729.973 kb on + strand, within BWI76_RS03520 at 729.973 kb on + strand, within BWI76_RS03520 at 729.981 kb on - strand, within BWI76_RS03520 at 729.981 kb on - strand, within BWI76_RS03520 at 729.981 kb on - strand, within BWI76_RS03520 at 729.984 kb on + strand, within BWI76_RS03520 at 729.984 kb on + strand, within BWI76_RS03520 at 729.984 kb on + strand, within BWI76_RS03520 at 729.999 kb on + strand, within BWI76_RS03520 at 730.026 kb on - strand, within BWI76_RS03520 at 730.087 kb on - strand, within BWI76_RS03520 at 730.134 kb on + strand, within BWI76_RS03520 at 730.203 kb on + strand, within BWI76_RS03520 at 730.231 kb on + strand, within BWI76_RS03520
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 727,281 + BWI76_RS03510 0.67 -1.5 727,485 - BWI76_RS03510 0.74 +0.3 727,494 - BWI76_RS03510 0.75 +0.3 727,809 - BWI76_RS03510 0.86 +0.7 727,859 + BWI76_RS03510 0.88 +1.0 727,933 - +1.6 727,957 + +0.2 728,050 + +0.2 728,073 + -1.5 728,089 + +1.6 728,121 - -0.5 728,259 + -0.1 728,275 + +0.5 728,283 - +1.0 728,393 + BWI76_RS03515 0.20 +1.1 728,439 - BWI76_RS03515 0.24 +0.8 728,660 + BWI76_RS03515 0.45 +0.6 729,171 + -0.5 729,267 - +0.3 729,273 + +0.0 729,278 + +0.2 729,347 - -0.8 729,347 - +0.5 729,469 + -0.1 729,471 + +1.2 729,479 - -1.5 729,528 + -1.7 729,536 - -0.1 729,579 + +0.5 729,579 + +0.4 729,587 + +0.7 729,589 + +0.5 729,595 - -0.5 729,595 - +0.8 729,631 - +0.2 729,646 - -2.7 729,646 - -0.0 729,654 - -0.2 729,658 + -0.1 729,659 + +0.9 729,667 - +2.0 729,667 - +1.0 729,713 + -0.3 729,713 + -0.1 729,720 - -1.2 729,721 - +0.2 729,721 - -0.5 729,726 + -1.6 729,726 + -0.0 729,732 - +3.6 729,734 + -0.7 729,734 - +0.2 729,744 - +0.7 729,757 + +0.1 729,757 + +1.8 729,757 + +0.4 729,759 + +0.0 729,759 + -1.1 729,759 + -1.3 729,759 + +0.4 729,765 - +0.3 729,767 - -0.3 729,767 - +0.3 729,767 - +0.1 729,772 - -0.6 729,835 + -1.2 729,835 + -0.8 729,835 + +0.2 729,835 + +0.0 729,835 + -0.6 729,835 + -2.8 729,835 + +0.3 729,835 + +0.2 729,835 + +1.2 729,835 + -0.0 729,835 + -0.6 729,835 + -0.1 729,835 + +0.2 729,835 + -0.4 729,835 + +0.2 729,837 + -1.3 729,839 + +1.2 729,843 - -0.1 729,843 - -0.7 729,843 - +0.2 729,843 - +0.1 729,843 - -1.3 729,843 - +0.0 729,843 - +0.1 729,843 - +0.7 729,843 - +0.2 729,843 - -1.3 729,843 - -0.1 729,843 - +0.4 729,843 - +1.2 729,845 + -0.7 729,845 + -0.6 729,845 + +0.5 729,845 + +0.2 729,845 + +1.3 729,845 + -1.5 729,845 - +2.2 729,845 - +2.6 729,853 - -1.1 729,853 - -0.6 729,853 - +0.1 729,853 - +0.4 729,853 - +0.7 729,853 - -0.7 729,853 - +0.7 729,853 - -0.9 729,857 + +1.1 729,858 + +1.2 729,858 + -0.3 729,858 + +0.0 729,858 + -0.1 729,858 + -0.8 729,858 + +1.8 729,858 + -2.6 729,859 - +0.1 729,861 - +0.7 729,866 - +0.2 729,866 - -0.5 729,878 + +0.3 729,878 - +1.0 729,894 + +0.7 729,895 + +0.5 729,901 - -1.2 729,955 + BWI76_RS03520 0.12 +0.7 729,973 + BWI76_RS03520 0.14 +2.6 729,973 + BWI76_RS03520 0.14 +2.0 729,973 + BWI76_RS03520 0.14 -0.1 729,981 - BWI76_RS03520 0.14 -0.1 729,981 - BWI76_RS03520 0.14 -1.6 729,981 - BWI76_RS03520 0.14 +0.1 729,984 + BWI76_RS03520 0.15 +1.0 729,984 + BWI76_RS03520 0.15 -0.4 729,984 + BWI76_RS03520 0.15 +0.8 729,999 + BWI76_RS03520 0.16 +0.1 730,026 - BWI76_RS03520 0.18 +0.1 730,087 - BWI76_RS03520 0.22 -0.1 730,134 + BWI76_RS03520 0.25 +0.9 730,203 + BWI76_RS03520 0.30 +1.1 730,231 + BWI76_RS03520 0.32 -2.5
Or see this region's nucleotide sequence