Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS03165 and BWI76_RS03170 are separated by 17 nucleotides BWI76_RS03170 and BWI76_RS03175 overlap by 4 nucleotides
BWI76_RS03165: BWI76_RS03165 - phage polarity suppression protein, at 654,232 to 654,798
_RS03165
BWI76_RS03170: BWI76_RS03170 - hypothetical protein, at 654,816 to 655,061
_RS03170
BWI76_RS03175: BWI76_RS03175 - hypothetical protein, at 655,058 to 655,795
_RS03175
Position (kb) 654
655
656 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 653.871 kb on + strand at 653.878 kb on + strand at 653.878 kb on + strand at 653.878 kb on - strand at 653.881 kb on + strand at 653.889 kb on - strand at 653.889 kb on - strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.892 kb on + strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.900 kb on - strand at 653.905 kb on - strand at 653.907 kb on + strand at 653.907 kb on + strand at 653.914 kb on + strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.915 kb on - strand at 653.922 kb on - strand at 653.923 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.925 kb on + strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.933 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.943 kb on - strand at 653.946 kb on - strand at 653.948 kb on - strand at 653.948 kb on - strand at 653.956 kb on - strand at 653.972 kb on - strand at 653.977 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 653.982 kb on - strand at 654.249 kb on - strand at 654.249 kb on - strand at 654.321 kb on + strand, within BWI76_RS03165 at 654.416 kb on - strand, within BWI76_RS03165 at 654.420 kb on + strand, within BWI76_RS03165 at 654.436 kb on + strand, within BWI76_RS03165 at 654.518 kb on - strand, within BWI76_RS03165 at 654.536 kb on - strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.663 kb on + strand, within BWI76_RS03165 at 654.671 kb on - strand, within BWI76_RS03165 at 654.671 kb on - strand, within BWI76_RS03165 at 654.674 kb on + strand, within BWI76_RS03165 at 654.682 kb on - strand, within BWI76_RS03165 at 654.807 kb on - strand at 654.843 kb on + strand, within BWI76_RS03170 at 654.932 kb on + strand, within BWI76_RS03170 at 654.940 kb on - strand, within BWI76_RS03170 at 654.940 kb on - strand, within BWI76_RS03170 at 655.324 kb on + strand, within BWI76_RS03175 at 655.344 kb on - strand, within BWI76_RS03175 at 655.377 kb on - strand, within BWI76_RS03175 at 655.421 kb on - strand, within BWI76_RS03175 at 655.481 kb on - strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.714 kb on + strand, within BWI76_RS03175 at 655.733 kb on + strand at 656.051 kb on + strand at 656.059 kb on - strand at 656.059 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 653,871 + -0.1 653,878 + -1.0 653,878 + +0.8 653,878 - -0.4 653,881 + -0.3 653,889 - -1.0 653,889 - +1.5 653,892 + +1.3 653,892 + -0.5 653,892 + +0.3 653,892 + +2.0 653,900 - +1.1 653,900 - +2.9 653,900 - -1.7 653,900 - -0.4 653,900 - -1.1 653,900 - -0.7 653,900 - +0.3 653,905 - -0.6 653,907 + +0.3 653,907 + -0.7 653,914 + +0.7 653,915 - -1.3 653,915 - -1.7 653,915 - -1.3 653,922 - +3.6 653,923 + +0.9 653,925 + +0.2 653,925 + -0.2 653,925 + -0.8 653,925 + +1.0 653,925 + -2.7 653,925 + +0.2 653,925 + -2.7 653,925 + -1.9 653,925 + +2.6 653,925 + -1.2 653,925 + +1.0 653,933 - +1.3 653,933 - -0.3 653,933 - +0.6 653,933 - -2.2 653,933 - -1.1 653,933 - -0.7 653,933 - -0.6 653,933 - +2.0 653,933 - +0.3 653,933 - -0.1 653,933 - -0.5 653,933 - -0.5 653,933 - +0.7 653,933 - +0.8 653,943 - -1.3 653,943 - -0.4 653,943 - +0.3 653,943 - -1.1 653,946 - -1.0 653,948 - -2.0 653,948 - +0.3 653,956 - +1.5 653,972 - -0.2 653,977 - -1.9 653,982 - +1.1 653,982 - -0.8 653,982 - +2.2 653,982 - +0.3 654,249 - -1.0 654,249 - -1.0 654,321 + BWI76_RS03165 0.16 -0.2 654,416 - BWI76_RS03165 0.32 +0.9 654,420 + BWI76_RS03165 0.33 -0.5 654,436 + BWI76_RS03165 0.36 -0.2 654,518 - BWI76_RS03165 0.50 +2.3 654,536 - BWI76_RS03165 0.54 -1.4 654,663 + BWI76_RS03165 0.76 +0.5 654,663 + BWI76_RS03165 0.76 -0.1 654,663 + BWI76_RS03165 0.76 +2.6 654,671 - BWI76_RS03165 0.77 -0.0 654,671 - BWI76_RS03165 0.77 -2.7 654,674 + BWI76_RS03165 0.78 +0.1 654,682 - BWI76_RS03165 0.79 -0.4 654,807 - -0.8 654,843 + BWI76_RS03170 0.11 -1.0 654,932 + BWI76_RS03170 0.47 -1.6 654,940 - BWI76_RS03170 0.50 -0.9 654,940 - BWI76_RS03170 0.50 -1.1 655,324 + BWI76_RS03175 0.36 -0.0 655,344 - BWI76_RS03175 0.39 -0.6 655,377 - BWI76_RS03175 0.43 -0.1 655,421 - BWI76_RS03175 0.49 -1.3 655,481 - BWI76_RS03175 0.57 -0.9 655,714 + BWI76_RS03175 0.89 +0.8 655,714 + BWI76_RS03175 0.89 -1.5 655,733 + +1.1 656,051 + -0.7 656,059 - -0.1 656,059 - -0.0
Or see this region's nucleotide sequence