Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02860 and BWI76_RS02865 are separated by 11 nucleotides BWI76_RS02865 and BWI76_RS02870 are separated by 2 nucleotides BWI76_RS02870 and BWI76_RS02875 are separated by 21 nucleotides BWI76_RS02875 and BWI76_RS02880 are separated by 12 nucleotides
BWI76_RS02860: BWI76_RS02860 - glycine dehydrogenase, at 586,022 to 586,360
_RS02860
BWI76_RS02865: BWI76_RS02865 - hypothetical protein, at 586,372 to 586,734
_RS02865
BWI76_RS02870: BWI76_RS02870 - hypothetical protein, at 586,737 to 587,036
_RS02870
BWI76_RS02875: BWI76_RS02875 - membrane protein, at 587,058 to 587,834
_RS02875
BWI76_RS02880: BWI76_RS02880 - membrane protein, at 587,847 to 588,491
_RS02880
Position (kb) 586
587
588 Strain fitness (log2 ratio) -1
0
1
2 at 585.981 kb on + strand at 585.981 kb on + strand at 586.011 kb on - strand at 586.055 kb on - strand at 586.112 kb on + strand, within BWI76_RS02860 at 586.296 kb on - strand, within BWI76_RS02860 at 586.357 kb on + strand at 586.410 kb on - strand, within BWI76_RS02865 at 586.553 kb on + strand, within BWI76_RS02865 at 586.553 kb on + strand, within BWI76_RS02865 at 586.560 kb on + strand, within BWI76_RS02865 at 586.566 kb on - strand, within BWI76_RS02865 at 586.639 kb on + strand, within BWI76_RS02865 at 586.654 kb on + strand, within BWI76_RS02865 at 586.704 kb on - strand at 586.819 kb on - strand, within BWI76_RS02870 at 586.881 kb on - strand, within BWI76_RS02870 at 586.963 kb on + strand at 587.404 kb on + strand, within BWI76_RS02875 at 587.446 kb on + strand, within BWI76_RS02875 at 587.615 kb on + strand, within BWI76_RS02875 at 587.640 kb on - strand, within BWI76_RS02875 at 587.645 kb on - strand, within BWI76_RS02875 at 587.670 kb on - strand, within BWI76_RS02875 at 587.810 kb on - strand at 587.863 kb on - strand at 587.886 kb on + strand at 587.914 kb on - strand, within BWI76_RS02880 at 587.942 kb on - strand, within BWI76_RS02880
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 585,981 + +0.5 585,981 + -1.3 586,011 - +0.4 586,055 - +0.6 586,112 + BWI76_RS02860 0.27 -1.0 586,296 - BWI76_RS02860 0.81 +0.7 586,357 + +0.8 586,410 - BWI76_RS02865 0.10 +1.3 586,553 + BWI76_RS02865 0.50 -1.7 586,553 + BWI76_RS02865 0.50 -0.3 586,560 + BWI76_RS02865 0.52 +0.4 586,566 - BWI76_RS02865 0.53 +0.8 586,639 + BWI76_RS02865 0.74 -0.1 586,654 + BWI76_RS02865 0.78 -0.7 586,704 - -0.2 586,819 - BWI76_RS02870 0.27 -0.4 586,881 - BWI76_RS02870 0.48 -1.4 586,963 + +1.3 587,404 + BWI76_RS02875 0.45 +2.7 587,446 + BWI76_RS02875 0.50 -0.8 587,615 + BWI76_RS02875 0.72 +0.4 587,640 - BWI76_RS02875 0.75 +0.1 587,645 - BWI76_RS02875 0.76 +0.6 587,670 - BWI76_RS02875 0.79 +0.2 587,810 - -0.5 587,863 - +0.2 587,886 + +0.3 587,914 - BWI76_RS02880 0.10 +1.3 587,942 - BWI76_RS02880 0.15 -1.0
Or see this region's nucleotide sequence