Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01570 and BWI76_RS01575 are separated by 9 nucleotides BWI76_RS01575 and BWI76_RS01580 are separated by 42 nucleotides BWI76_RS01580 and BWI76_RS01585 are separated by 186 nucleotides BWI76_RS01585 and BWI76_RS01590 are separated by 12 nucleotides
BWI76_RS01570: BWI76_RS01570 - uroporphyrinogen decarboxylase, at 319,579 to 320,643
_RS01570
BWI76_RS01575: BWI76_RS01575 - endonuclease V, at 320,653 to 321,324
_RS01575
BWI76_RS01580: BWI76_RS01580 - hypothetical protein, at 321,367 to 321,957
_RS01580
BWI76_RS01585: BWI76_RS01585 - transcriptional regulator, at 322,144 to 322,416
_RS01585
BWI76_RS01590: BWI76_RS01590 - hypothetical protein, at 322,429 to 323,121
_RS01590
Position (kb) 321
322 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 320.637 kb on + strand at 320.803 kb on + strand, within BWI76_RS01575 at 320.805 kb on + strand, within BWI76_RS01575 at 320.873 kb on - strand, within BWI76_RS01575 at 320.918 kb on - strand, within BWI76_RS01575 at 321.111 kb on + strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.201 kb on - strand, within BWI76_RS01575 at 321.210 kb on + strand, within BWI76_RS01575 at 321.211 kb on - strand, within BWI76_RS01575 at 321.246 kb on + strand, within BWI76_RS01575 at 321.263 kb on + strand at 321.617 kb on + strand, within BWI76_RS01580 at 321.625 kb on - strand, within BWI76_RS01580 at 321.888 kb on + strand, within BWI76_RS01580 at 322.181 kb on + strand, within BWI76_RS01585 at 322.181 kb on + strand at 322.189 kb on - strand, within BWI76_RS01585 at 322.220 kb on + strand, within BWI76_RS01585 at 322.289 kb on + strand, within BWI76_RS01585 at 322.305 kb on - strand, within BWI76_RS01585 at 322.310 kb on - strand, within BWI76_RS01585 at 322.329 kb on + strand, within BWI76_RS01585 at 322.419 kb on + strand at 322.419 kb on - strand at 322.608 kb on - strand, within BWI76_RS01590 at 322.640 kb on - strand, within BWI76_RS01590 at 322.756 kb on - strand, within BWI76_RS01590 at 322.772 kb on + strand, within BWI76_RS01590 at 322.772 kb on + strand, within BWI76_RS01590 at 322.780 kb on - strand, within BWI76_RS01590 at 322.952 kb on + strand, within BWI76_RS01590
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 320,637 + +1.2 320,803 + BWI76_RS01575 0.22 -0.8 320,805 + BWI76_RS01575 0.23 -1.7 320,873 - BWI76_RS01575 0.33 +0.2 320,918 - BWI76_RS01575 0.39 -1.3 321,111 + BWI76_RS01575 0.68 +0.1 321,201 - BWI76_RS01575 0.82 -1.7 321,201 - BWI76_RS01575 0.82 -0.1 321,201 - BWI76_RS01575 0.82 +0.1 321,201 - BWI76_RS01575 0.82 -0.4 321,210 + BWI76_RS01575 0.83 -0.2 321,211 - BWI76_RS01575 0.83 -0.9 321,246 + BWI76_RS01575 0.88 +0.1 321,263 + +0.1 321,617 + BWI76_RS01580 0.42 -0.7 321,625 - BWI76_RS01580 0.44 -0.3 321,888 + BWI76_RS01580 0.88 -3.1 322,181 + BWI76_RS01585 0.14 +1.6 322,181 + +0.1 322,189 - BWI76_RS01585 0.16 +1.0 322,220 + BWI76_RS01585 0.28 +1.1 322,289 + BWI76_RS01585 0.53 +0.3 322,305 - BWI76_RS01585 0.59 +1.4 322,310 - BWI76_RS01585 0.61 +1.2 322,329 + BWI76_RS01585 0.68 +0.4 322,419 + +0.9 322,419 - +0.9 322,608 - BWI76_RS01590 0.26 +0.6 322,640 - BWI76_RS01590 0.30 +0.7 322,756 - BWI76_RS01590 0.47 -0.1 322,772 + BWI76_RS01590 0.49 -1.7 322,772 + BWI76_RS01590 0.49 +0.5 322,780 - BWI76_RS01590 0.51 -0.6 322,952 + BWI76_RS01590 0.75 -0.9
Or see this region's nucleotide sequence