Experiment: LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01130 and BWI76_RS01135 are separated by 11 nucleotides BWI76_RS01135 and BWI76_RS01140 are separated by 240 nucleotides
BWI76_RS01130: BWI76_RS01130 - enterobacterial common antigen polymerase, at 232,899 to 234,245
_RS01130
BWI76_RS01135: BWI76_RS01135 - lipopolysaccharide N-acetylmannosaminouronosyltransferase, at 234,257 to 234,997
_RS01135
BWI76_RS01140: BWI76_RS01140 - amino acid permease, at 235,238 to 236,623
_RS01140
Position (kb) 234
235 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 234.278 kb on - strand at 234.297 kb on - strand at 234.607 kb on + strand, within BWI76_RS01135 at 234.644 kb on - strand, within BWI76_RS01135 at 234.795 kb on + strand, within BWI76_RS01135 at 234.820 kb on + strand, within BWI76_RS01135 at 234.820 kb on + strand, within BWI76_RS01135 at 234.820 kb on + strand, within BWI76_RS01135 at 234.828 kb on - strand, within BWI76_RS01135 at 234.839 kb on + strand, within BWI76_RS01135 at 234.986 kb on + strand at 235.121 kb on + strand at 235.121 kb on + strand at 235.121 kb on + strand at 235.124 kb on - strand at 235.129 kb on - strand at 235.183 kb on - strand at 235.185 kb on + strand at 235.188 kb on - strand at 235.200 kb on - strand at 235.210 kb on - strand at 235.216 kb on - strand at 235.216 kb on - strand at 235.216 kb on - strand at 235.218 kb on + strand at 235.227 kb on - strand at 235.298 kb on + strand at 235.346 kb on + strand at 235.585 kb on + strand, within BWI76_RS01140 at 235.701 kb on + strand, within BWI76_RS01140 at 235.710 kb on - strand, within BWI76_RS01140 at 235.742 kb on - strand, within BWI76_RS01140 at 235.755 kb on + strand, within BWI76_RS01140 at 235.869 kb on + strand, within BWI76_RS01140
Per-strain Table
Position Strand Gene LocusTag Fraction LB 0.5x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^6 spores/mL remove 234,278 - -0.7 234,297 - +1.3 234,607 + BWI76_RS01135 0.47 -1.7 234,644 - BWI76_RS01135 0.52 -0.7 234,795 + BWI76_RS01135 0.73 -2.4 234,820 + BWI76_RS01135 0.76 -1.0 234,820 + BWI76_RS01135 0.76 -2.6 234,820 + BWI76_RS01135 0.76 -4.4 234,828 - BWI76_RS01135 0.77 -0.7 234,839 + BWI76_RS01135 0.79 +1.3 234,986 + -0.8 235,121 + +0.9 235,121 + -0.4 235,121 + +0.1 235,124 - -0.7 235,129 - +0.9 235,183 - +2.6 235,185 + -1.0 235,188 - -0.1 235,200 - -0.4 235,210 - +2.9 235,216 - +0.4 235,216 - -0.1 235,216 - +1.3 235,218 + +0.2 235,227 - -0.9 235,298 + +0.4 235,346 + +0.2 235,585 + BWI76_RS01140 0.25 -0.2 235,701 + BWI76_RS01140 0.33 -0.7 235,710 - BWI76_RS01140 0.34 +0.9 235,742 - BWI76_RS01140 0.36 -0.1 235,755 + BWI76_RS01140 0.37 +0.5 235,869 + BWI76_RS01140 0.46 +0.7
Or see this region's nucleotide sequence