Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS26235 and BWI76_RS26240 overlap by 1 nucleotides BWI76_RS26240 and BWI76_RS26245 are separated by 180 nucleotides BWI76_RS26245 and BWI76_RS26250 are separated by 114 nucleotides BWI76_RS26250 and BWI76_RS26255 are separated by 121 nucleotides
BWI76_RS26235: BWI76_RS26235 - gluconate transporter, at 5,431,582 to 5,432,922
_RS26235
BWI76_RS26240: BWI76_RS26240 - gluconokinase, at 5,432,922 to 5,433,452
_RS26240
BWI76_RS26245: BWI76_RS26245 - transcriptional regulator, at 5,433,633 to 5,434,628
_RS26245
BWI76_RS26250: BWI76_RS26250 - quercetin 2,3-dioxygenase, at 5,434,743 to 5,435,438
_RS26250
BWI76_RS26255: BWI76_RS26255 - oxidoreductase, at 5,435,560 to 5,436,597
_RS26255
Position (kb) 5433
5434
5435 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5432.953 kb on - strand at 5433.773 kb on - strand, within BWI76_RS26245 at 5433.900 kb on - strand, within BWI76_RS26245 at 5434.022 kb on + strand, within BWI76_RS26245 at 5434.203 kb on - strand, within BWI76_RS26245 at 5434.220 kb on + strand, within BWI76_RS26245 at 5434.826 kb on + strand, within BWI76_RS26250 at 5434.863 kb on + strand, within BWI76_RS26250 at 5434.933 kb on - strand, within BWI76_RS26250 at 5435.013 kb on + strand, within BWI76_RS26250 at 5435.019 kb on + strand, within BWI76_RS26250 at 5435.048 kb on - strand, within BWI76_RS26250 at 5435.132 kb on - strand, within BWI76_RS26250 at 5435.132 kb on - strand, within BWI76_RS26250 at 5435.142 kb on - strand, within BWI76_RS26250 at 5435.399 kb on - strand at 5435.539 kb on + strand at 5435.595 kb on + strand at 5435.595 kb on + strand at 5435.595 kb on + strand at 5435.603 kb on - strand at 5435.624 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 5,432,953 - +0.3 5,433,773 - BWI76_RS26245 0.14 -1.5 5,433,900 - BWI76_RS26245 0.27 -0.7 5,434,022 + BWI76_RS26245 0.39 -2.3 5,434,203 - BWI76_RS26245 0.57 -0.3 5,434,220 + BWI76_RS26245 0.59 -1.0 5,434,826 + BWI76_RS26250 0.12 +0.6 5,434,863 + BWI76_RS26250 0.17 -0.1 5,434,933 - BWI76_RS26250 0.27 +0.3 5,435,013 + BWI76_RS26250 0.39 -0.4 5,435,019 + BWI76_RS26250 0.40 +1.0 5,435,048 - BWI76_RS26250 0.44 -0.8 5,435,132 - BWI76_RS26250 0.56 -0.0 5,435,132 - BWI76_RS26250 0.56 +0.3 5,435,142 - BWI76_RS26250 0.57 +0.3 5,435,399 - -0.1 5,435,539 + +1.9 5,435,595 + +0.8 5,435,595 + +0.4 5,435,595 + -0.2 5,435,603 - +0.3 5,435,624 - -0.2
Or see this region's nucleotide sequence