Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS25520 and BWI76_RS25525 are separated by 121 nucleotides BWI76_RS25525 and BWI76_RS25530 are separated by 9 nucleotides BWI76_RS25530 and BWI76_RS25535 overlap by 28 nucleotides BWI76_RS25535 and BWI76_RS25540 are separated by 10 nucleotides BWI76_RS25540 and BWI76_RS25545 are separated by 110 nucleotides BWI76_RS25545 and BWI76_RS25550 overlap by 11 nucleotides
BWI76_RS25520: BWI76_RS25520 - sulfoxide reductase heme-binding subunit YedZ, at 5,306,066 to 5,306,665
_RS25520
BWI76_RS25525: BWI76_RS25525 - hypothetical protein, at 5,306,787 to 5,306,987
_RS25525
BWI76_RS25530: BWI76_RS25530 - acetyl-CoA carboxylase, at 5,306,997 to 5,307,086
_RS25530
BWI76_RS25535: BWI76_RS25535 - acetyl-CoA carboxylase biotin carboxyl carrier protein subunit, at 5,307,059 to 5,307,532
_RS25535
BWI76_RS25540: BWI76_RS25540 - acetyl-CoA carboxylase biotin carboxylase subunit, at 5,307,543 to 5,308,892
_RS25540
BWI76_RS25545: BWI76_RS25545 - hypothetical protein, at 5,309,003 to 5,309,245
_RS25545
BWI76_RS25550: BWI76_RS25550 - sodium:pantothenate symporter, at 5,309,235 to 5,310,686
_RS25550
Position (kb) 5307
5308
5309 Strain fitness (log2 ratio) -2
-1
0
1
2 at 5306.575 kb on + strand, within BWI76_RS25520 at 5308.950 kb on + strand at 5309.026 kb on - strand at 5309.031 kb on - strand, within BWI76_RS25545 at 5309.136 kb on + strand, within BWI76_RS25545 at 5309.182 kb on + strand, within BWI76_RS25545 at 5309.192 kb on + strand, within BWI76_RS25545 at 5309.235 kb on - strand at 5309.294 kb on - strand at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.405 kb on - strand, within BWI76_RS25550 at 5309.744 kb on + strand, within BWI76_RS25550 at 5309.745 kb on + strand, within BWI76_RS25550 at 5309.747 kb on + strand, within BWI76_RS25550 at 5309.799 kb on + strand, within BWI76_RS25550 at 5309.807 kb on - strand, within BWI76_RS25550
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 5,306,575 + BWI76_RS25520 0.85 +1.2 5,308,950 + -0.3 5,309,026 - +1.3 5,309,031 - BWI76_RS25545 0.12 +1.2 5,309,136 + BWI76_RS25545 0.55 +1.8 5,309,182 + BWI76_RS25545 0.74 +0.3 5,309,192 + BWI76_RS25545 0.78 +1.2 5,309,235 - +0.0 5,309,294 - -0.7 5,309,405 - BWI76_RS25550 0.12 +0.2 5,309,405 - BWI76_RS25550 0.12 +1.4 5,309,405 - BWI76_RS25550 0.12 -1.1 5,309,744 + BWI76_RS25550 0.35 -0.5 5,309,745 + BWI76_RS25550 0.35 +0.2 5,309,747 + BWI76_RS25550 0.35 +1.0 5,309,799 + BWI76_RS25550 0.39 -0.6 5,309,807 - BWI76_RS25550 0.39 -2.1
Or see this region's nucleotide sequence