Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21695 and BWI76_RS21700 are separated by 665 nucleotides BWI76_RS21700 and BWI76_RS21705 are separated by 87 nucleotides
BWI76_RS21695: BWI76_RS21695 - glycosyl transferase family protein, at 4,536,958 to 4,537,947
_RS21695
BWI76_RS21700: BWI76_RS21700 - AraC family transcriptional regulator, at 4,538,613 to 4,539,407
_RS21700
BWI76_RS21705: BWI76_RS21705 - MFS transporter, at 4,539,495 to 4,540,685
_RS21705
Position (kb) 4538
4539
4540 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4537.623 kb on + strand, within BWI76_RS21695 at 4537.771 kb on - strand, within BWI76_RS21695 at 4537.776 kb on + strand, within BWI76_RS21695 at 4537.789 kb on + strand, within BWI76_RS21695 at 4537.877 kb on - strand at 4537.879 kb on - strand at 4537.879 kb on - strand at 4537.884 kb on - strand at 4537.899 kb on - strand at 4537.917 kb on + strand at 4537.984 kb on + strand at 4537.992 kb on - strand at 4538.052 kb on + strand at 4538.062 kb on + strand at 4538.070 kb on - strand at 4538.070 kb on - strand at 4538.096 kb on + strand at 4538.127 kb on + strand at 4538.151 kb on + strand at 4538.164 kb on - strand at 4538.217 kb on + strand at 4538.328 kb on - strand at 4538.395 kb on + strand at 4538.395 kb on + strand at 4538.403 kb on + strand at 4538.403 kb on - strand at 4538.409 kb on + strand at 4538.417 kb on - strand at 4538.417 kb on - strand at 4538.424 kb on + strand at 4538.426 kb on - strand at 4538.432 kb on - strand at 4538.432 kb on - strand at 4538.432 kb on - strand at 4538.432 kb on - strand at 4538.611 kb on + strand at 4538.652 kb on - strand at 4538.662 kb on - strand at 4538.667 kb on - strand at 4538.703 kb on + strand, within BWI76_RS21700 at 4538.710 kb on + strand, within BWI76_RS21700 at 4538.715 kb on + strand, within BWI76_RS21700 at 4538.718 kb on + strand, within BWI76_RS21700 at 4538.723 kb on - strand, within BWI76_RS21700 at 4538.820 kb on + strand, within BWI76_RS21700 at 4538.849 kb on - strand, within BWI76_RS21700 at 4538.857 kb on - strand, within BWI76_RS21700 at 4538.895 kb on - strand, within BWI76_RS21700 at 4539.045 kb on + strand, within BWI76_RS21700 at 4539.168 kb on + strand, within BWI76_RS21700 at 4539.250 kb on - strand, within BWI76_RS21700 at 4539.369 kb on - strand at 4539.369 kb on - strand at 4539.374 kb on + strand at 4539.510 kb on + strand at 4539.626 kb on + strand, within BWI76_RS21705 at 4539.636 kb on - strand, within BWI76_RS21705 at 4539.682 kb on + strand, within BWI76_RS21705 at 4539.725 kb on - strand, within BWI76_RS21705 at 4539.725 kb on - strand, within BWI76_RS21705 at 4539.775 kb on + strand, within BWI76_RS21705 at 4539.788 kb on + strand, within BWI76_RS21705 at 4539.834 kb on + strand, within BWI76_RS21705 at 4539.842 kb on - strand, within BWI76_RS21705 at 4539.853 kb on + strand, within BWI76_RS21705 at 4539.943 kb on + strand, within BWI76_RS21705 at 4539.979 kb on - strand, within BWI76_RS21705 at 4540.116 kb on + strand, within BWI76_RS21705 at 4540.139 kb on + strand, within BWI76_RS21705 at 4540.212 kb on - strand, within BWI76_RS21705 at 4540.239 kb on - strand, within BWI76_RS21705 at 4540.404 kb on - strand, within BWI76_RS21705
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 4,537,623 + BWI76_RS21695 0.67 +0.1 4,537,771 - BWI76_RS21695 0.82 -0.0 4,537,776 + BWI76_RS21695 0.83 -0.7 4,537,789 + BWI76_RS21695 0.84 -0.3 4,537,877 - -0.2 4,537,879 - +0.6 4,537,879 - +0.3 4,537,884 - -1.2 4,537,899 - +0.8 4,537,917 + -2.2 4,537,984 + +0.5 4,537,992 - -1.6 4,538,052 + -0.4 4,538,062 + -1.0 4,538,070 - -1.3 4,538,070 - -1.2 4,538,096 + -2.2 4,538,127 + +1.7 4,538,151 + -0.1 4,538,164 - -2.5 4,538,217 + -3.1 4,538,328 - -1.5 4,538,395 + -0.5 4,538,395 + -0.3 4,538,403 + -0.5 4,538,403 - -1.3 4,538,409 + -0.1 4,538,417 - -1.9 4,538,417 - -1.2 4,538,424 + -0.5 4,538,426 - -0.3 4,538,432 - -0.5 4,538,432 - -0.7 4,538,432 - -3.0 4,538,432 - -0.2 4,538,611 + +0.2 4,538,652 - -0.5 4,538,662 - -1.9 4,538,667 - -1.0 4,538,703 + BWI76_RS21700 0.11 +0.4 4,538,710 + BWI76_RS21700 0.12 +0.2 4,538,715 + BWI76_RS21700 0.13 -3.0 4,538,718 + BWI76_RS21700 0.13 +0.5 4,538,723 - BWI76_RS21700 0.14 +3.3 4,538,820 + BWI76_RS21700 0.26 +2.2 4,538,849 - BWI76_RS21700 0.30 -0.0 4,538,857 - BWI76_RS21700 0.31 +0.5 4,538,895 - BWI76_RS21700 0.35 +0.3 4,539,045 + BWI76_RS21700 0.54 -0.1 4,539,168 + BWI76_RS21700 0.70 +0.2 4,539,250 - BWI76_RS21700 0.80 -1.0 4,539,369 - -1.4 4,539,369 - -0.7 4,539,374 + -1.5 4,539,510 + -0.7 4,539,626 + BWI76_RS21705 0.11 -0.1 4,539,636 - BWI76_RS21705 0.12 +2.3 4,539,682 + BWI76_RS21705 0.16 -2.7 4,539,725 - BWI76_RS21705 0.19 -2.0 4,539,725 - BWI76_RS21705 0.19 -0.6 4,539,775 + BWI76_RS21705 0.24 -0.3 4,539,788 + BWI76_RS21705 0.25 -0.2 4,539,834 + BWI76_RS21705 0.28 -0.3 4,539,842 - BWI76_RS21705 0.29 -0.6 4,539,853 + BWI76_RS21705 0.30 +0.3 4,539,943 + BWI76_RS21705 0.38 +0.2 4,539,979 - BWI76_RS21705 0.41 +0.9 4,540,116 + BWI76_RS21705 0.52 -0.3 4,540,139 + BWI76_RS21705 0.54 -0.3 4,540,212 - BWI76_RS21705 0.60 -0.4 4,540,239 - BWI76_RS21705 0.62 -2.2 4,540,404 - BWI76_RS21705 0.76 -0.0
Or see this region's nucleotide sequence