Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21390 and BWI76_RS21395 are separated by 69 nucleotides BWI76_RS21395 and BWI76_RS21400 are separated by 35 nucleotides BWI76_RS21400 and BWI76_RS21405 are separated by 93 nucleotides
BWI76_RS21390: BWI76_RS21390 - thiol disulfide reductase thioredoxin, at 4,477,662 to 4,478,087
_RS21390
BWI76_RS21395: BWI76_RS21395 - DTW domain-containing protein, at 4,478,157 to 4,478,855
_RS21395
BWI76_RS21400: BWI76_RS21400 - protein lysine acetyltransferase, at 4,478,891 to 4,481,569
_RS21400
BWI76_RS21405: BWI76_RS21405 - phosphatidylserine synthase, at 4,481,663 to 4,483,018
_RS21405
Position (kb) 4478
4479
4480
4481
4482 Strain fitness (log2 ratio) -2
-1
0
1 at 4477.899 kb on + strand, within BWI76_RS21390 at 4477.936 kb on - strand, within BWI76_RS21390 at 4478.299 kb on - strand, within BWI76_RS21395 at 4478.651 kb on + strand, within BWI76_RS21395 at 4478.789 kb on + strand at 4478.813 kb on + strand at 4478.874 kb on + strand at 4479.064 kb on + strand at 4479.262 kb on + strand, within BWI76_RS21400 at 4479.267 kb on + strand, within BWI76_RS21400 at 4479.275 kb on - strand, within BWI76_RS21400 at 4479.437 kb on + strand, within BWI76_RS21400 at 4479.445 kb on - strand, within BWI76_RS21400 at 4479.520 kb on + strand, within BWI76_RS21400 at 4479.694 kb on + strand, within BWI76_RS21400 at 4479.859 kb on - strand, within BWI76_RS21400 at 4480.091 kb on - strand, within BWI76_RS21400 at 4480.295 kb on - strand, within BWI76_RS21400 at 4480.340 kb on + strand, within BWI76_RS21400 at 4480.845 kb on + strand, within BWI76_RS21400 at 4480.930 kb on - strand, within BWI76_RS21400 at 4481.019 kb on + strand, within BWI76_RS21400 at 4481.022 kb on - strand, within BWI76_RS21400 at 4481.086 kb on + strand, within BWI76_RS21400 at 4481.112 kb on - strand, within BWI76_RS21400 at 4481.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 4,477,899 + BWI76_RS21390 0.56 -0.2 4,477,936 - BWI76_RS21390 0.64 +0.9 4,478,299 - BWI76_RS21395 0.20 +0.3 4,478,651 + BWI76_RS21395 0.71 -1.8 4,478,789 + +0.9 4,478,813 + -0.4 4,478,874 + +0.2 4,479,064 + -0.5 4,479,262 + BWI76_RS21400 0.14 +0.2 4,479,267 + BWI76_RS21400 0.14 -0.3 4,479,275 - BWI76_RS21400 0.14 -1.9 4,479,437 + BWI76_RS21400 0.20 +0.3 4,479,445 - BWI76_RS21400 0.21 +0.3 4,479,520 + BWI76_RS21400 0.23 +0.5 4,479,694 + BWI76_RS21400 0.30 -0.6 4,479,859 - BWI76_RS21400 0.36 +0.5 4,480,091 - BWI76_RS21400 0.45 -1.3 4,480,295 - BWI76_RS21400 0.52 -1.4 4,480,340 + BWI76_RS21400 0.54 -0.7 4,480,845 + BWI76_RS21400 0.73 -0.9 4,480,930 - BWI76_RS21400 0.76 -1.1 4,481,019 + BWI76_RS21400 0.79 -0.9 4,481,022 - BWI76_RS21400 0.80 +1.1 4,481,086 + BWI76_RS21400 0.82 -0.4 4,481,112 - BWI76_RS21400 0.83 +1.3 4,481,470 + +0.7
Or see this region's nucleotide sequence