Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20205 and BWI76_RS20210 are separated by 87 nucleotides BWI76_RS20210 and BWI76_RS20215 are separated by 53 nucleotides BWI76_RS20215 and BWI76_RS20220 are separated by 76 nucleotides BWI76_RS20220 and BWI76_RS20225 are separated by 11 nucleotides
BWI76_RS20205: BWI76_RS20205 - aminotransferase AlaT, at 4,234,466 to 4,235,683
_RS20205
BWI76_RS20210: BWI76_RS20210 - 5'-deoxynucleotidase, at 4,235,771 to 4,236,370
_RS20210
BWI76_RS20215: BWI76_RS20215 - transcriptional regulator, at 4,236,424 to 4,238,256
_RS20215
BWI76_RS20220: BWI76_RS20220 - sugar phosphatase, at 4,238,333 to 4,238,992
_RS20220
BWI76_RS20225: BWI76_RS20225 - hypothetical protein, at 4,239,004 to 4,239,498
_RS20225
Position (kb) 4236
4237
4238
4239 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4235.442 kb on - strand, within BWI76_RS20205 at 4235.459 kb on + strand, within BWI76_RS20205 at 4235.459 kb on + strand, within BWI76_RS20205 at 4235.757 kb on + strand at 4235.757 kb on + strand at 4235.872 kb on - strand, within BWI76_RS20210 at 4235.935 kb on + strand, within BWI76_RS20210 at 4235.943 kb on - strand, within BWI76_RS20210 at 4235.943 kb on - strand, within BWI76_RS20210 at 4235.943 kb on - strand, within BWI76_RS20210 at 4236.053 kb on - strand, within BWI76_RS20210 at 4236.285 kb on + strand, within BWI76_RS20210 at 4236.290 kb on + strand, within BWI76_RS20210 at 4236.390 kb on + strand at 4236.458 kb on + strand at 4236.619 kb on - strand, within BWI76_RS20215 at 4236.684 kb on - strand, within BWI76_RS20215 at 4236.859 kb on + strand, within BWI76_RS20215 at 4237.010 kb on - strand, within BWI76_RS20215 at 4237.167 kb on - strand, within BWI76_RS20215 at 4237.265 kb on + strand, within BWI76_RS20215 at 4237.357 kb on - strand, within BWI76_RS20215 at 4237.658 kb on + strand, within BWI76_RS20215 at 4237.666 kb on - strand, within BWI76_RS20215 at 4237.921 kb on + strand, within BWI76_RS20215 at 4237.930 kb on + strand, within BWI76_RS20215 at 4237.967 kb on - strand, within BWI76_RS20215 at 4238.082 kb on + strand at 4238.124 kb on + strand at 4238.156 kb on + strand at 4238.231 kb on - strand at 4238.251 kb on + strand at 4238.558 kb on - strand, within BWI76_RS20220 at 4238.720 kb on - strand, within BWI76_RS20220 at 4238.738 kb on - strand, within BWI76_RS20220 at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.979 kb on + strand at 4238.987 kb on - strand at 4239.045 kb on - strand at 4239.045 kb on - strand at 4239.226 kb on + strand, within BWI76_RS20225
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 4,235,442 - BWI76_RS20205 0.80 +1.2 4,235,459 + BWI76_RS20205 0.82 -0.4 4,235,459 + BWI76_RS20205 0.82 +0.0 4,235,757 + -1.1 4,235,757 + -0.6 4,235,872 - BWI76_RS20210 0.17 +0.5 4,235,935 + BWI76_RS20210 0.27 +1.1 4,235,943 - BWI76_RS20210 0.29 +0.4 4,235,943 - BWI76_RS20210 0.29 +0.7 4,235,943 - BWI76_RS20210 0.29 -0.4 4,236,053 - BWI76_RS20210 0.47 +1.7 4,236,285 + BWI76_RS20210 0.86 +0.9 4,236,290 + BWI76_RS20210 0.86 +1.2 4,236,390 + -1.0 4,236,458 + +1.2 4,236,619 - BWI76_RS20215 0.11 +0.2 4,236,684 - BWI76_RS20215 0.14 -0.8 4,236,859 + BWI76_RS20215 0.24 -0.7 4,237,010 - BWI76_RS20215 0.32 +0.2 4,237,167 - BWI76_RS20215 0.41 +0.8 4,237,265 + BWI76_RS20215 0.46 +1.7 4,237,357 - BWI76_RS20215 0.51 +1.8 4,237,658 + BWI76_RS20215 0.67 -0.7 4,237,666 - BWI76_RS20215 0.68 -0.1 4,237,921 + BWI76_RS20215 0.82 -0.8 4,237,930 + BWI76_RS20215 0.82 -1.2 4,237,967 - BWI76_RS20215 0.84 +2.7 4,238,082 + +1.4 4,238,124 + -0.4 4,238,156 + +0.4 4,238,231 - +1.1 4,238,251 + -0.2 4,238,558 - BWI76_RS20220 0.34 +0.3 4,238,720 - BWI76_RS20220 0.59 +0.7 4,238,738 - BWI76_RS20220 0.61 -0.0 4,238,979 + +0.8 4,238,979 + +0.4 4,238,979 + +0.6 4,238,979 + -0.1 4,238,987 - +0.5 4,239,045 - +0.9 4,239,045 - -0.6 4,239,226 + BWI76_RS20225 0.45 -2.6
Or see this region's nucleotide sequence