Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20170 and BWI76_RS20175 overlap by 4 nucleotides BWI76_RS20175 and BWI76_RS20180 are separated by 2 nucleotides BWI76_RS20180 and BWI76_RS20185 are separated by 98 nucleotides BWI76_RS20185 and BWI76_RS20190 are separated by 15 nucleotides
BWI76_RS20170: BWI76_RS20170 - NADH-quinone oxidoreductase subunit F, at 4,227,130 to 4,228,467
_RS20170
BWI76_RS20175: BWI76_RS20175 - NADH-quinone oxidoreductase subunit E, at 4,228,464 to 4,228,964
_RS20175
BWI76_RS20180: BWI76_RS20180 - NADH-quinone oxidoreductase subunit C/D, at 4,228,967 to 4,230,763
_RS20180
BWI76_RS20185: BWI76_RS20185 - NADH-quinone oxidoreductase subunit B, at 4,230,862 to 4,231,536
_RS20185
BWI76_RS20190: BWI76_RS20190 - NADH-quinone oxidoreductase subunit A, at 4,231,552 to 4,231,998
_RS20190
Position (kb) 4228
4229
4230
4231 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 4228.008 kb on - strand, within BWI76_RS20170 at 4228.295 kb on - strand, within BWI76_RS20170 at 4228.438 kb on + strand at 4228.521 kb on - strand, within BWI76_RS20175 at 4229.509 kb on - strand, within BWI76_RS20180 at 4229.533 kb on + strand, within BWI76_RS20180 at 4229.573 kb on - strand, within BWI76_RS20180 at 4229.753 kb on + strand, within BWI76_RS20180 at 4230.095 kb on + strand, within BWI76_RS20180 at 4230.096 kb on - strand, within BWI76_RS20180 at 4230.263 kb on - strand, within BWI76_RS20180 at 4230.307 kb on + strand, within BWI76_RS20180 at 4230.870 kb on + strand at 4231.087 kb on + strand, within BWI76_RS20185 at 4231.302 kb on - strand at 4231.557 kb on + strand at 4231.565 kb on - strand at 4231.591 kb on - strand at 4231.657 kb on - strand, within BWI76_RS20190
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 4,228,008 - BWI76_RS20170 0.66 -1.2 4,228,295 - BWI76_RS20170 0.87 -0.2 4,228,438 + -1.2 4,228,521 - BWI76_RS20175 0.11 -0.8 4,229,509 - BWI76_RS20180 0.30 -1.4 4,229,533 + BWI76_RS20180 0.31 -1.6 4,229,573 - BWI76_RS20180 0.34 -2.8 4,229,753 + BWI76_RS20180 0.44 +0.2 4,230,095 + BWI76_RS20180 0.63 -1.4 4,230,096 - BWI76_RS20180 0.63 -4.0 4,230,263 - BWI76_RS20180 0.72 -0.9 4,230,307 + BWI76_RS20180 0.75 -1.6 4,230,870 + +1.2 4,231,087 + BWI76_RS20185 0.33 -0.8 4,231,302 - +0.5 4,231,557 + -0.1 4,231,565 - -1.9 4,231,591 - +0.3 4,231,657 - BWI76_RS20190 0.23 -1.3
Or see this region's nucleotide sequence