Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS16050 and BWI76_RS16055 overlap by 4 nucleotides BWI76_RS16055 and BWI76_RS16060 overlap by 11 nucleotides BWI76_RS16060 and BWI76_RS16065 are separated by 215 nucleotides
BWI76_RS16050: BWI76_RS16050 - malonate decarboxylase holo-ACP synthase, at 3,327,649 to 3,328,269
_RS16050
BWI76_RS16055: BWI76_RS16055 - malonate decarboxylase subunit epsilon, at 3,328,266 to 3,329,168
_RS16055
BWI76_RS16060: BWI76_RS16060 - LysR family transcriptional regulator, at 3,329,158 to 3,330,084
_RS16060
BWI76_RS16065: BWI76_RS16065 - malate:quinone oxidoreductase, at 3,330,300 to 3,331,955
_RS16065
Position (kb) 3329
3330
3331 Strain fitness (log2 ratio) -2
-1
0
1 at 3328.433 kb on - strand, within BWI76_RS16055 at 3328.607 kb on + strand, within BWI76_RS16055 at 3328.703 kb on + strand, within BWI76_RS16055 at 3328.905 kb on + strand, within BWI76_RS16055 at 3328.913 kb on - strand, within BWI76_RS16055 at 3328.942 kb on - strand, within BWI76_RS16055 at 3329.424 kb on + strand, within BWI76_RS16060 at 3329.432 kb on - strand, within BWI76_RS16060 at 3329.522 kb on + strand, within BWI76_RS16060 at 3329.564 kb on + strand, within BWI76_RS16060 at 3329.756 kb on - strand, within BWI76_RS16060 at 3329.948 kb on - strand at 3330.467 kb on + strand, within BWI76_RS16065 at 3330.538 kb on - strand, within BWI76_RS16065 at 3330.736 kb on - strand, within BWI76_RS16065 at 3330.745 kb on - strand, within BWI76_RS16065 at 3330.791 kb on + strand, within BWI76_RS16065 at 3330.793 kb on - strand, within BWI76_RS16065 at 3330.815 kb on + strand, within BWI76_RS16065 at 3330.830 kb on + strand, within BWI76_RS16065 at 3330.939 kb on - strand at 3331.006 kb on + strand, within BWI76_RS16065 at 3331.039 kb on + strand, within BWI76_RS16065 at 3331.047 kb on - strand, within BWI76_RS16065 at 3331.052 kb on - strand, within BWI76_RS16065 at 3331.056 kb on + strand, within BWI76_RS16065 at 3331.078 kb on - strand, within BWI76_RS16065
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,328,433 - BWI76_RS16055 0.18 +0.1 3,328,607 + BWI76_RS16055 0.38 +1.2 3,328,703 + BWI76_RS16055 0.48 +1.2 3,328,905 + BWI76_RS16055 0.71 +0.9 3,328,913 - BWI76_RS16055 0.72 +1.7 3,328,942 - BWI76_RS16055 0.75 +0.6 3,329,424 + BWI76_RS16060 0.29 +0.5 3,329,432 - BWI76_RS16060 0.30 -0.5 3,329,522 + BWI76_RS16060 0.39 -0.4 3,329,564 + BWI76_RS16060 0.44 +0.2 3,329,756 - BWI76_RS16060 0.65 +0.3 3,329,948 - +1.5 3,330,467 + BWI76_RS16065 0.10 +1.0 3,330,538 - BWI76_RS16065 0.14 -0.3 3,330,736 - BWI76_RS16065 0.26 -1.1 3,330,745 - BWI76_RS16065 0.27 +0.2 3,330,791 + BWI76_RS16065 0.30 +0.9 3,330,793 - BWI76_RS16065 0.30 +0.8 3,330,815 + BWI76_RS16065 0.31 -2.4 3,330,830 + BWI76_RS16065 0.32 -2.1 3,330,939 - +0.5 3,331,006 + BWI76_RS16065 0.43 +0.1 3,331,039 + BWI76_RS16065 0.45 -0.4 3,331,047 - BWI76_RS16065 0.45 -0.1 3,331,052 - BWI76_RS16065 0.45 +0.1 3,331,056 + BWI76_RS16065 0.46 -2.3 3,331,078 - BWI76_RS16065 0.47 +0.2
Or see this region's nucleotide sequence