Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15895 and BWI76_RS15900 are separated by 208 nucleotides BWI76_RS15900 and BWI76_RS15905 are separated by 136 nucleotides BWI76_RS15905 and BWI76_RS15910 are separated by 60 nucleotides BWI76_RS15910 and BWI76_RS15915 overlap by 7 nucleotides
BWI76_RS15895: BWI76_RS15895 - starvation-sensing protein RspA, at 3,298,205 to 3,299,419
_RS15895
BWI76_RS15900: BWI76_RS15900 - hypothetical protein, at 3,299,628 to 3,299,953
_RS15900
BWI76_RS15905: BWI76_RS15905 - hypothetical protein, at 3,300,090 to 3,300,431
_RS15905
BWI76_RS15910: BWI76_RS15910 - spermidine acetyltransferase, at 3,300,492 to 3,301,052
_RS15910
BWI76_RS15915: BWI76_RS15915 - YnfC family lipoprotein, at 3,301,046 to 3,301,756
_RS15915
Position (kb)
3300
3301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3299.171 kb on + strand, within BWI76_RS15895 at 3299.265 kb on - strand, within BWI76_RS15895 at 3299.275 kb on - strand, within BWI76_RS15895 at 3299.327 kb on - strand at 3299.536 kb on - strand at 3299.569 kb on - strand at 3299.633 kb on - strand at 3299.744 kb on - strand, within BWI76_RS15900 at 3299.781 kb on - strand, within BWI76_RS15900 at 3300.394 kb on + strand, within BWI76_RS15905 at 3300.402 kb on - strand at 3300.402 kb on - strand at 3300.545 kb on - strand at 3300.545 kb on - strand at 3300.847 kb on + strand, within BWI76_RS15910 at 3301.015 kb on - strand at 3301.038 kb on + strand at 3301.082 kb on - strand at 3301.114 kb on - strand at 3301.123 kb on + strand, within BWI76_RS15915 at 3301.234 kb on + strand, within BWI76_RS15915
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,299,171 + BWI76_RS15895 0.80 +1.0 3,299,265 - BWI76_RS15895 0.87 +0.1 3,299,275 - BWI76_RS15895 0.88 +0.7 3,299,327 - -1.4 3,299,536 - -0.1 3,299,569 - +0.3 3,299,633 - -0.7 3,299,744 - BWI76_RS15900 0.36 +1.3 3,299,781 - BWI76_RS15900 0.47 +0.3 3,300,394 + BWI76_RS15905 0.89 +4.9 3,300,402 - +0.5 3,300,402 - +0.1 3,300,545 - +0.7 3,300,545 - +0.3 3,300,847 + BWI76_RS15910 0.63 -0.0 3,301,015 - -0.3 3,301,038 + +0.6 3,301,082 - +1.6 3,301,114 - -1.8 3,301,123 + BWI76_RS15915 0.11 +0.0 3,301,234 + BWI76_RS15915 0.26 -0.0
Or see this region's nucleotide sequence