Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14790 and BWI76_RS14795 are separated by 146 nucleotides BWI76_RS14795 and BWI76_RS14800 are separated by 45 nucleotides BWI76_RS14800 and BWI76_RS14805 are separated by 302 nucleotides BWI76_RS14805 and BWI76_RS14810 are separated by 4 nucleotides
BWI76_RS14790: BWI76_RS14790 - 2-dehydropantoate 2-reductase, at 3,101,209 to 3,102,102
_RS14790
BWI76_RS14795: BWI76_RS14795 - hypothetical protein, at 3,102,249 to 3,102,473
_RS14795
BWI76_RS14800: BWI76_RS14800 - non-heme chloroperoxidase, at 3,102,519 to 3,103,355
_RS14800
BWI76_RS14805: BWI76_RS14805 - hypothetical protein, at 3,103,658 to 3,104,092
_RS14805
BWI76_RS14810: BWI76_RS14810 - carboxymuconolactone decarboxylase, at 3,104,097 to 3,104,897
_RS14810
Position (kb) 3102
3103
3104 Strain fitness (log2 ratio) -2
-1
0
1
2 at 3101.607 kb on + strand, within BWI76_RS14790 at 3102.083 kb on - strand at 3102.467 kb on + strand at 3102.467 kb on + strand at 3102.832 kb on - strand, within BWI76_RS14800 at 3102.995 kb on - strand, within BWI76_RS14800 at 3103.092 kb on - strand, within BWI76_RS14800 at 3103.173 kb on + strand, within BWI76_RS14800 at 3103.295 kb on + strand at 3103.430 kb on + strand at 3103.595 kb on - strand at 3103.841 kb on + strand, within BWI76_RS14805 at 3103.955 kb on + strand, within BWI76_RS14805 at 3104.076 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 3,101,607 + BWI76_RS14790 0.45 -0.9 3,102,083 - +0.2 3,102,467 + -0.1 3,102,467 + +0.7 3,102,832 - BWI76_RS14800 0.37 +0.1 3,102,995 - BWI76_RS14800 0.57 +1.5 3,103,092 - BWI76_RS14800 0.68 -1.8 3,103,173 + BWI76_RS14800 0.78 -0.2 3,103,295 + +0.5 3,103,430 + +0.1 3,103,595 - +1.9 3,103,841 + BWI76_RS14805 0.42 +1.2 3,103,955 + BWI76_RS14805 0.68 +2.0 3,104,076 - +0.8
Or see this region's nucleotide sequence