Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13090 and BWI76_RS13095 are separated by 11 nucleotides BWI76_RS13095 and BWI76_RS13100 are separated by 7 nucleotides BWI76_RS13100 and BWI76_RS13105 are separated by 12 nucleotides BWI76_RS13105 and BWI76_RS13110 are separated by 7 nucleotides
BWI76_RS13090: BWI76_RS13090 - phenylacetate-CoA oxygenase subunit PaaA, at 2,731,790 to 2,732,719
_RS13090
BWI76_RS13095: BWI76_RS13095 - phenylacetate-CoA oxygenase subunit PaaB, at 2,732,731 to 2,733,018
_RS13095
BWI76_RS13100: BWI76_RS13100 - phenylacetate-CoA oxygenase subunit PaaI, at 2,733,026 to 2,733,781
_RS13100
BWI76_RS13105: BWI76_RS13105 - phenylacetate-CoA oxygenase subunit PaaJ, at 2,733,794 to 2,734,291
_RS13105
BWI76_RS13110: BWI76_RS13110 - phenylacetic acid degradation protein, at 2,734,299 to 2,735,369
_RS13110
Position (kb)
2733
2734 Strain fitness (log2 ratio)
-2
-1
0
1 at 2732.123 kb on + strand, within BWI76_RS13090 at 2732.224 kb on - strand, within BWI76_RS13090 at 2732.879 kb on + strand, within BWI76_RS13095 at 2732.982 kb on + strand, within BWI76_RS13095 at 2732.990 kb on - strand at 2733.007 kb on + strand at 2733.042 kb on - strand at 2733.362 kb on + strand, within BWI76_RS13100 at 2733.402 kb on + strand, within BWI76_RS13100 at 2733.453 kb on + strand, within BWI76_RS13100 at 2733.541 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.580 kb on + strand, within BWI76_RS13100 at 2733.765 kb on - strand at 2734.083 kb on + strand, within BWI76_RS13105 at 2734.176 kb on - strand, within BWI76_RS13105 at 2734.235 kb on + strand, within BWI76_RS13105 at 2734.454 kb on + strand, within BWI76_RS13110
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,732,123 + BWI76_RS13090 0.36 -1.4 2,732,224 - BWI76_RS13090 0.47 +1.1 2,732,879 + BWI76_RS13095 0.51 +0.7 2,732,982 + BWI76_RS13095 0.87 +0.0 2,732,990 - -0.5 2,733,007 + +0.6 2,733,042 - +1.1 2,733,362 + BWI76_RS13100 0.44 -1.0 2,733,402 + BWI76_RS13100 0.50 -0.5 2,733,453 + BWI76_RS13100 0.56 +0.3 2,733,541 + BWI76_RS13100 0.68 -2.2 2,733,580 + BWI76_RS13100 0.73 -2.2 2,733,580 + BWI76_RS13100 0.73 -1.1 2,733,765 - +0.3 2,734,083 + BWI76_RS13105 0.58 +0.8 2,734,176 - BWI76_RS13105 0.77 -1.2 2,734,235 + BWI76_RS13105 0.89 +1.5 2,734,454 + BWI76_RS13110 0.14 +1.3
Or see this region's nucleotide sequence