Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12845 and BWI76_RS12850 are separated by 169 nucleotides BWI76_RS12850 and BWI76_RS12855 are separated by 23 nucleotides BWI76_RS12855 and BWI76_RS12860 overlap by 4 nucleotides
BWI76_RS12845: BWI76_RS12845 - LysR family transcriptional regulator, at 2,677,385 to 2,678,302
_RS12845
BWI76_RS12850: BWI76_RS12850 - hypothetical protein, at 2,678,472 to 2,679,731
_RS12850
BWI76_RS12855: BWI76_RS12855 - enamine deaminase RidA, at 2,679,755 to 2,680,189
_RS12855
BWI76_RS12860: BWI76_RS12860 - hypothetical protein, at 2,680,186 to 2,680,860
_RS12860
Position (kb)
2678
2679
2680 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2677.491 kb on + strand, within BWI76_RS12845 at 2677.563 kb on - strand, within BWI76_RS12845 at 2677.565 kb on - strand, within BWI76_RS12845 at 2677.565 kb on - strand, within BWI76_RS12845 at 2677.570 kb on - strand, within BWI76_RS12845 at 2677.768 kb on - strand, within BWI76_RS12845 at 2677.800 kb on - strand, within BWI76_RS12845 at 2677.800 kb on - strand, within BWI76_RS12845 at 2677.849 kb on + strand at 2677.873 kb on + strand at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.891 kb on + strand, within BWI76_RS12845 at 2677.899 kb on - strand, within BWI76_RS12845 at 2677.899 kb on - strand, within BWI76_RS12845 at 2677.900 kb on - strand, within BWI76_RS12845 at 2677.911 kb on + strand, within BWI76_RS12845 at 2677.919 kb on - strand, within BWI76_RS12845 at 2677.952 kb on + strand, within BWI76_RS12845 at 2677.952 kb on + strand, within BWI76_RS12845 at 2677.952 kb on + strand, within BWI76_RS12845 at 2677.959 kb on + strand, within BWI76_RS12845 at 2677.959 kb on + strand, within BWI76_RS12845 at 2677.960 kb on - strand, within BWI76_RS12845 at 2677.967 kb on - strand, within BWI76_RS12845 at 2677.967 kb on - strand at 2677.983 kb on + strand, within BWI76_RS12845 at 2678.037 kb on + strand, within BWI76_RS12845 at 2678.047 kb on + strand, within BWI76_RS12845 at 2678.047 kb on + strand at 2678.047 kb on + strand, within BWI76_RS12845 at 2678.047 kb on + strand, within BWI76_RS12845 at 2678.048 kb on + strand, within BWI76_RS12845 at 2678.048 kb on + strand, within BWI76_RS12845 at 2678.055 kb on - strand, within BWI76_RS12845 at 2678.064 kb on + strand, within BWI76_RS12845 at 2678.080 kb on + strand, within BWI76_RS12845 at 2678.082 kb on + strand, within BWI76_RS12845 at 2678.088 kb on - strand, within BWI76_RS12845 at 2678.088 kb on - strand, within BWI76_RS12845 at 2678.095 kb on + strand, within BWI76_RS12845 at 2678.096 kb on + strand, within BWI76_RS12845 at 2678.096 kb on + strand at 2678.104 kb on - strand at 2678.104 kb on - strand, within BWI76_RS12845 at 2678.122 kb on - strand, within BWI76_RS12845 at 2678.135 kb on + strand, within BWI76_RS12845 at 2678.151 kb on + strand, within BWI76_RS12845 at 2678.296 kb on + strand at 2678.339 kb on + strand at 2678.339 kb on + strand at 2678.339 kb on + strand at 2678.339 kb on + strand at 2678.339 kb on + strand at 2678.352 kb on - strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.407 kb on + strand at 2678.412 kb on + strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.415 kb on - strand at 2678.422 kb on + strand at 2678.430 kb on - strand at 2678.445 kb on + strand at 2678.449 kb on - strand at 2678.453 kb on - strand at 2678.455 kb on + strand at 2678.462 kb on + strand at 2678.464 kb on + strand at 2678.468 kb on + strand at 2678.476 kb on - strand at 2678.478 kb on + strand at 2678.481 kb on - strand at 2678.492 kb on + strand at 2678.498 kb on - strand at 2678.508 kb on - strand at 2678.508 kb on - strand at 2678.509 kb on - strand at 2678.512 kb on + strand at 2678.512 kb on + strand at 2678.566 kb on - strand at 2678.600 kb on + strand, within BWI76_RS12850 at 2678.601 kb on + strand, within BWI76_RS12850 at 2678.668 kb on - strand, within BWI76_RS12850 at 2678.783 kb on + strand, within BWI76_RS12850 at 2678.928 kb on - strand, within BWI76_RS12850 at 2678.950 kb on + strand, within BWI76_RS12850 at 2679.059 kb on - strand, within BWI76_RS12850 at 2679.126 kb on + strand, within BWI76_RS12850 at 2679.228 kb on - strand, within BWI76_RS12850 at 2679.241 kb on + strand, within BWI76_RS12850 at 2679.249 kb on - strand, within BWI76_RS12850 at 2679.347 kb on + strand, within BWI76_RS12850 at 2679.389 kb on - strand, within BWI76_RS12850 at 2679.769 kb on - strand at 2679.837 kb on - strand, within BWI76_RS12855 at 2680.044 kb on + strand, within BWI76_RS12855 at 2680.303 kb on + strand, within BWI76_RS12860 at 2680.518 kb on - strand, within BWI76_RS12860
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 2,677,491 + BWI76_RS12845 0.12 +0.5 2,677,563 - BWI76_RS12845 0.19 +1.1 2,677,565 - BWI76_RS12845 0.20 +0.7 2,677,565 - BWI76_RS12845 0.20 -0.5 2,677,570 - BWI76_RS12845 0.20 +1.5 2,677,768 - BWI76_RS12845 0.42 -1.7 2,677,800 - BWI76_RS12845 0.45 -0.5 2,677,800 - BWI76_RS12845 0.45 -2.3 2,677,849 + +0.5 2,677,873 + +1.5 2,677,891 + BWI76_RS12845 0.55 +0.5 2,677,891 + BWI76_RS12845 0.55 -0.5 2,677,891 + +2.7 2,677,891 + BWI76_RS12845 0.55 -0.4 2,677,891 + BWI76_RS12845 0.55 -0.0 2,677,891 + BWI76_RS12845 0.55 -2.4 2,677,891 + BWI76_RS12845 0.55 +0.8 2,677,891 + BWI76_RS12845 0.55 -1.6 2,677,891 + BWI76_RS12845 0.55 -0.1 2,677,899 - BWI76_RS12845 0.56 +1.5 2,677,899 - BWI76_RS12845 0.56 -1.1 2,677,900 - BWI76_RS12845 0.56 -0.1 2,677,911 + BWI76_RS12845 0.57 -1.6 2,677,919 - BWI76_RS12845 0.58 -2.1 2,677,952 + BWI76_RS12845 0.62 +0.1 2,677,952 + BWI76_RS12845 0.62 +0.5 2,677,952 + BWI76_RS12845 0.62 -2.3 2,677,959 + BWI76_RS12845 0.63 -3.0 2,677,959 + BWI76_RS12845 0.63 -0.5 2,677,960 - BWI76_RS12845 0.63 -1.5 2,677,967 - BWI76_RS12845 0.63 -2.4 2,677,967 - -2.3 2,677,983 + BWI76_RS12845 0.65 -1.1 2,678,037 + BWI76_RS12845 0.71 -1.8 2,678,047 + BWI76_RS12845 0.72 -0.2 2,678,047 + +0.5 2,678,047 + BWI76_RS12845 0.72 +1.5 2,678,047 + BWI76_RS12845 0.72 -0.6 2,678,048 + BWI76_RS12845 0.72 +0.5 2,678,048 + BWI76_RS12845 0.72 +0.1 2,678,055 - BWI76_RS12845 0.73 -0.9 2,678,064 + BWI76_RS12845 0.74 -0.5 2,678,080 + BWI76_RS12845 0.76 +2.3 2,678,082 + BWI76_RS12845 0.76 -0.5 2,678,088 - BWI76_RS12845 0.77 -0.8 2,678,088 - BWI76_RS12845 0.77 +1.0 2,678,095 + BWI76_RS12845 0.77 +1.9 2,678,096 + BWI76_RS12845 0.77 +1.8 2,678,096 + -0.1 2,678,104 - +1.5 2,678,104 - BWI76_RS12845 0.78 -1.8 2,678,122 - BWI76_RS12845 0.80 +0.2 2,678,135 + BWI76_RS12845 0.82 -0.1 2,678,151 + BWI76_RS12845 0.83 +0.3 2,678,296 + -0.8 2,678,339 + -1.7 2,678,339 + -1.8 2,678,339 + -2.2 2,678,339 + +1.5 2,678,339 + -0.1 2,678,352 - +0.1 2,678,407 + -1.7 2,678,407 + -1.1 2,678,407 + +1.7 2,678,407 + +1.0 2,678,407 + -0.9 2,678,407 + +0.6 2,678,407 + +1.5 2,678,407 + -0.5 2,678,407 + +0.4 2,678,407 + +0.8 2,678,407 + +1.7 2,678,407 + -0.4 2,678,407 + +0.5 2,678,407 + +1.5 2,678,407 + -1.7 2,678,412 + -0.9 2,678,415 - -0.9 2,678,415 - +0.2 2,678,415 - -1.2 2,678,415 - -1.1 2,678,415 - -1.1 2,678,415 - -1.7 2,678,415 - -0.1 2,678,415 - +0.2 2,678,415 - -0.3 2,678,415 - +1.3 2,678,415 - -0.5 2,678,415 - -0.3 2,678,422 + -0.1 2,678,430 - -2.9 2,678,445 + +0.2 2,678,449 - +0.1 2,678,453 - -0.3 2,678,455 + +0.3 2,678,462 + -0.1 2,678,464 + +0.4 2,678,468 + +0.8 2,678,476 - -0.1 2,678,478 + -1.4 2,678,481 - -0.0 2,678,492 + -0.1 2,678,498 - -0.1 2,678,508 - -1.3 2,678,508 - +0.5 2,678,509 - -2.4 2,678,512 + +0.2 2,678,512 + -3.1 2,678,566 - +0.9 2,678,600 + BWI76_RS12850 0.10 -1.1 2,678,601 + BWI76_RS12850 0.10 -0.4 2,678,668 - BWI76_RS12850 0.16 +1.1 2,678,783 + BWI76_RS12850 0.25 +0.1 2,678,928 - BWI76_RS12850 0.36 +1.0 2,678,950 + BWI76_RS12850 0.38 +0.6 2,679,059 - BWI76_RS12850 0.47 +0.0 2,679,126 + BWI76_RS12850 0.52 -0.3 2,679,228 - BWI76_RS12850 0.60 +1.8 2,679,241 + BWI76_RS12850 0.61 +0.5 2,679,249 - BWI76_RS12850 0.62 -0.4 2,679,347 + BWI76_RS12850 0.69 +0.7 2,679,389 - BWI76_RS12850 0.73 +0.5 2,679,769 - +0.8 2,679,837 - BWI76_RS12855 0.19 -0.0 2,680,044 + BWI76_RS12855 0.66 -0.2 2,680,303 + BWI76_RS12860 0.17 -0.6 2,680,518 - BWI76_RS12860 0.49 +1.1
Or see this region's nucleotide sequence