Experiment: LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08960 and BWI76_RS08965 are separated by 110 nucleotides BWI76_RS08965 and BWI76_RS08970 overlap by 4 nucleotides BWI76_RS08970 and BWI76_RS08975 are separated by 134 nucleotides
BWI76_RS08960: BWI76_RS08960 - mechanosensitive channel protein, at 1,887,562 to 1,889,772
_RS08960
BWI76_RS08965: BWI76_RS08965 - glutamine ABC transporter ATP-binding protein, at 1,889,883 to 1,890,605
_RS08965
BWI76_RS08970: BWI76_RS08970 - glutamine ABC transporter permease GlnP, at 1,890,602 to 1,891,261
_RS08970
BWI76_RS08975: BWI76_RS08975 - amino acid ABC transporter substrate-binding protein, at 1,891,396 to 1,892,142
_RS08975
Position (kb) 1890
1891
1892 Strain fitness (log2 ratio) -1
0
1 at 1889.803 kb on + strand at 1889.912 kb on + strand at 1890.342 kb on + strand, within BWI76_RS08965 at 1890.421 kb on - strand, within BWI76_RS08965 at 1890.461 kb on + strand, within BWI76_RS08965 at 1890.469 kb on - strand, within BWI76_RS08965 at 1890.474 kb on + strand, within BWI76_RS08965 at 1890.487 kb on - strand, within BWI76_RS08965 at 1890.645 kb on - strand at 1890.973 kb on - strand, within BWI76_RS08970 at 1891.266 kb on - strand at 1891.266 kb on - strand at 1891.275 kb on - strand at 1891.379 kb on - strand at 1891.499 kb on - strand, within BWI76_RS08975 at 1891.553 kb on + strand, within BWI76_RS08975 at 1891.944 kb on + strand, within BWI76_RS08975 at 1891.981 kb on + strand, within BWI76_RS08975 at 1891.994 kb on - strand, within BWI76_RS08975 at 1892.126 kb on - strand at 1892.145 kb on - strand at 1892.167 kb on + strand at 1892.167 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB 1x with Mixed culture; Trichoderma harzianum M10 spores at 2.5*10^4 spores/mL remove 1,889,803 + +0.4 1,889,912 + +0.8 1,890,342 + BWI76_RS08965 0.63 -1.1 1,890,421 - BWI76_RS08965 0.74 +0.7 1,890,461 + BWI76_RS08965 0.80 -0.3 1,890,469 - BWI76_RS08965 0.81 +0.5 1,890,474 + BWI76_RS08965 0.82 +0.4 1,890,487 - BWI76_RS08965 0.84 -1.6 1,890,645 - +0.1 1,890,973 - BWI76_RS08970 0.56 +0.9 1,891,266 - -0.3 1,891,266 - +0.6 1,891,275 - -0.3 1,891,379 - +0.1 1,891,499 - BWI76_RS08975 0.14 +0.6 1,891,553 + BWI76_RS08975 0.21 +0.9 1,891,944 + BWI76_RS08975 0.73 +0.3 1,891,981 + BWI76_RS08975 0.78 +0.3 1,891,994 - BWI76_RS08975 0.80 +0.3 1,892,126 - +1.0 1,892,145 - +0.7 1,892,167 + +0.9 1,892,167 + +1.3
Or see this region's nucleotide sequence